+Open data
-Basic information
Entry | Database: PDB / ID: 5ovv | ||||||
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Title | PDZ domain from rat Shank3 bound to the C terminus of ProSAPiP1 | ||||||
Components |
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Keywords | PROTEIN BINDING / PDZ domain / peptide binding / post-synaptic density / C terminus | ||||||
Function / homology | Function and homology information response to interleukin-17 / regulation of AMPA glutamate receptor clustering / guanylate kinase-associated protein clustering / striatal medium spiny neuron differentiation / synaptic receptor adaptor activity / maintenance of postsynaptic density structure / RET signaling / positive regulation of glutamate receptor signaling pathway / postsynaptic density assembly / embryonic epithelial tube formation ...response to interleukin-17 / regulation of AMPA glutamate receptor clustering / guanylate kinase-associated protein clustering / striatal medium spiny neuron differentiation / synaptic receptor adaptor activity / maintenance of postsynaptic density structure / RET signaling / positive regulation of glutamate receptor signaling pathway / postsynaptic density assembly / embryonic epithelial tube formation / Neurexins and neuroligins / positive regulation of synapse structural plasticity / negative regulation of actin filament bundle assembly / vocal learning / negative regulation of cell volume / positive regulation of long-term neuronal synaptic plasticity / structural constituent of postsynaptic density / regulation of grooming behavior / NMDA glutamate receptor clustering / vocalization behavior / regulation of dendritic spine morphogenesis / regulation of behavioral fear response / neuron spine / AMPA glutamate receptor clustering / neural precursor cell proliferation / locomotion / dendritic spine morphogenesis / brain morphogenesis / regulation of long-term synaptic potentiation / positive regulation of AMPA receptor activity / long-term synaptic depression / regulation of postsynapse organization / ciliary membrane / exploration behavior / regulation of long-term synaptic depression / adult behavior / positive regulation of dendritic spine development / locomotory exploration behavior / postsynaptic density, intracellular component / social behavior / associative learning / positive regulation of excitatory postsynaptic potential / neuromuscular process controlling balance / glial cell proliferation / synapse assembly / ionotropic glutamate receptor binding / positive regulation of synaptic transmission, glutamatergic / locomotory behavior / learning / long-term synaptic potentiation / positive regulation of long-term synaptic potentiation / PDZ domain binding / G protein-coupled receptor binding / regulation of synaptic plasticity / modulation of chemical synaptic transmission / protein homooligomerization / memory / SH3 domain binding / : / MAPK cascade / gene expression / actin binding / scaffold protein binding / dendritic spine / postsynaptic density / learning or memory / cytoskeleton / neuron projection / synapse / glutamatergic synapse / protein-containing complex binding / zinc ion binding / identical protein binding / plasma membrane / cytoplasm Similarity search - Function | ||||||
Biological species | Rattus norvegicus (Norway rat) | ||||||
Method | X-RAY DIFFRACTION / SYNCHROTRON / Resolution: 1.4 Å | ||||||
Authors | Ponna, S.K. / Myllykoski, M. / Boeckers, T.M. / Kursula, P. | ||||||
Citation | Journal: J. Neurochem. / Year: 2018 Title: Structural basis for PDZ domain interactions in the post-synaptic density scaffolding protein Shank3. Authors: Ponna, S.K. / Ruskamo, S. / Myllykoski, M. / Keller, C. / Boeckers, T.M. / Kursula, P. | ||||||
History |
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-Structure visualization
Structure viewer | Molecule: MolmilJmol/JSmol |
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-Downloads & links
-Download
PDBx/mmCIF format | 5ovv.cif.gz | 79.6 KB | Display | PDBx/mmCIF format |
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PDB format | pdb5ovv.ent.gz | 63.9 KB | Display | PDB format |
PDBx/mmJSON format | 5ovv.json.gz | Tree view | PDBx/mmJSON format | |
Others | Other downloads |
-Validation report
Arichive directory | https://data.pdbj.org/pub/pdb/validation_reports/ov/5ovv ftp://data.pdbj.org/pub/pdb/validation_reports/ov/5ovv | HTTPS FTP |
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-Related structure data
-Links
-Assembly
Deposited unit |
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1 |
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Unit cell |
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-Components
#1: Protein | Mass: 13576.530 Da / Num. of mol.: 1 Source method: isolated from a genetically manipulated source Source: (gene. exp.) Rattus norvegicus (Norway rat) / Gene: Shank3 / Production host: Escherichia coli (E. coli) / References: UniProt: A0A0U1RRP5, UniProt: Q9JLU4*PLUS | ||
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#2: Protein/peptide | Mass: 716.778 Da / Num. of mol.: 1 / Source method: obtained synthetically / Source: (synth.) Rattus norvegicus (Norway rat) / References: UniProt: Q8K1Q4 | ||
#3: Chemical | #4: Water | ChemComp-HOH / | |
-Experimental details
-Experiment
Experiment | Method: X-RAY DIFFRACTION / Number of used crystals: 1 |
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-Sample preparation
Crystal | Density Matthews: 2.53 Å3/Da / Density % sol: 51.37 % |
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Crystal grow | Temperature: 293 K / Method: vapor diffusion, sitting drop / Details: Sodium thiocyanate 200 mM, 23% PEG3350 |
-Data collection
Diffraction | Mean temperature: 100 K |
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Diffraction source | Source: SYNCHROTRON / Site: PETRA III, EMBL c/o DESY / Beamline: P13 (MX1) / Wavelength: 1.0332 Å |
Detector | Type: DECTRIS PILATUS3 6M / Detector: PIXEL / Date: Oct 1, 2016 |
Radiation | Protocol: SINGLE WAVELENGTH / Monochromatic (M) / Laue (L): M / Scattering type: x-ray |
Radiation wavelength | Wavelength: 1.0332 Å / Relative weight: 1 |
Reflection | Resolution: 1.4→50 Å / Num. obs: 22464 / % possible obs: 99.8 % / Redundancy: 12.6 % / Biso Wilson estimate: 20 Å2 / CC1/2: 0.996 / Rrim(I) all: 0.218 / Net I/σ(I): 8.2 |
Reflection shell | Resolution: 1.4→1.44 Å / Redundancy: 11.5 % / Num. unique obs: 1644 / CC1/2: 0.333 / Rrim(I) all: 2.177 / % possible all: 99.2 |
-Processing
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Refinement | Resolution: 1.4→42.955 Å / SU ML: 0.17 / Cross valid method: FREE R-VALUE / σ(F): 1.34 / Phase error: 22.1 / Stereochemistry target values: ML
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Solvent computation | Shrinkage radii: 0.9 Å / VDW probe radii: 1.11 Å / Solvent model: FLAT BULK SOLVENT MODEL | |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Refinement step | Cycle: LAST / Resolution: 1.4→42.955 Å
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Refine LS restraints |
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LS refinement shell |
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