+Open data
-Basic information
Entry | Database: PDB / ID: 5noi | ||||||
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Title | human Robo2 extracellular domains 4-5 | ||||||
Components | Roundabout homolog 2 | ||||||
Keywords | SIGNALING PROTEIN / Slit-Robo | ||||||
Function / homology | Function and homology information olfactory bulb interneuron development / apoptotic process involved in luteolysis / negative regulation of negative chemotaxis / Regulation of cortical dendrite branching / negative regulation of synapse assembly / heart induction / ROBO receptors bind AKAP5 / axon guidance receptor activity / Regulation of commissural axon pathfinding by SLIT and ROBO / endocardial cushion formation ...olfactory bulb interneuron development / apoptotic process involved in luteolysis / negative regulation of negative chemotaxis / Regulation of cortical dendrite branching / negative regulation of synapse assembly / heart induction / ROBO receptors bind AKAP5 / axon guidance receptor activity / Regulation of commissural axon pathfinding by SLIT and ROBO / endocardial cushion formation / Signaling by ROBO receptors / pulmonary valve morphogenesis / outflow tract septum morphogenesis / metanephros development / aortic valve morphogenesis / retinal ganglion cell axon guidance / axon midline choice point recognition / aorta development / ureteric bud development / positive regulation of axonogenesis / ventricular septum morphogenesis / positive regulation of Notch signaling pathway / homophilic cell adhesion via plasma membrane adhesion molecules / axolemma / cellular response to hormone stimulus / central nervous system development / axon guidance / brain development / cell-cell adhesion / Regulation of expression of SLITs and ROBOs / chemotaxis / cell surface / extracellular exosome / identical protein binding / plasma membrane Similarity search - Function | ||||||
Biological species | Homo sapiens (human) | ||||||
Method | X-RAY DIFFRACTION / SYNCHROTRON / MOLECULAR REPLACEMENT / Resolution: 2.4 Å | ||||||
Authors | Opatowsky, Y. / Barak, R. | ||||||
Funding support | Israel, 1items
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Citation | Journal: J. Mol. Biol. / Year: 2017 Title: Robo Ig4 Is a Dimerization Domain. Authors: Yom-Tov, G. / Barak, R. / Matalon, O. / Barda-Saad, M. / Guez-Haddad, J. / Opatowsky, Y. | ||||||
History |
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-Structure visualization
Structure viewer | Molecule: MolmilJmol/JSmol |
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-Downloads & links
-Download
PDBx/mmCIF format | 5noi.cif.gz | 89.7 KB | Display | PDBx/mmCIF format |
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PDB format | pdb5noi.ent.gz | 68.6 KB | Display | PDB format |
PDBx/mmJSON format | 5noi.json.gz | Tree view | PDBx/mmJSON format | |
Others | Other downloads |
-Validation report
Arichive directory | https://data.pdbj.org/pub/pdb/validation_reports/no/5noi ftp://data.pdbj.org/pub/pdb/validation_reports/no/5noi | HTTPS FTP |
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-Related structure data
-Links
-Assembly
Deposited unit |
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1 |
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Unit cell |
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-Components
#1: Protein | Mass: 21687.525 Da / Num. of mol.: 1 Source method: isolated from a genetically manipulated source Source: (gene. exp.) Homo sapiens (human) / Gene: ROBO2, KIAA1568 / Production host: Escherichia coli BL21 (bacteria) / References: UniProt: Q9HCK4 |
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#2: Water | ChemComp-HOH / |
-Experimental details
-Experiment
Experiment | Method: X-RAY DIFFRACTION / Number of used crystals: 1 |
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-Sample preparation
Crystal | Density Matthews: 3.19 Å3/Da / Density % sol: 65.26 % / Description: pyramid |
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Crystal grow | Temperature: 277 K / Method: vapor diffusion, hanging drop / Details: 1.1M NaCl, 11-13% PEG 6000 |
-Data collection
Diffraction | Mean temperature: 100 K |
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Diffraction source | Source: SYNCHROTRON / Site: ESRF / Beamline: ID30B / Wavelength: 1.008818 Å |
Detector | Type: DECTRIS PILATUS 6M-F / Detector: PIXEL / Date: Jun 29, 2015 |
Radiation | Protocol: SINGLE WAVELENGTH / Monochromatic (M) / Laue (L): M / Scattering type: x-ray |
Radiation wavelength | Wavelength: 1.008818 Å / Relative weight: 1 |
Reflection | Resolution: 2.4→38.8 Å / Num. obs: 12530 / % possible obs: 100 % / Redundancy: 12.5 % / Net I/σ(I): 21.6 |
-Processing
Software |
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Refinement | Method to determine structure: MOLECULAR REPLACEMENT Starting model: 4C4K, 2EDJ Resolution: 2.4→38.8 Å / SU ML: 0.47 / Cross valid method: FREE R-VALUE / σ(F): 1.35 / Phase error: 33.17
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Solvent computation | Shrinkage radii: 0.9 Å / VDW probe radii: 1.11 Å | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Refinement step | Cycle: LAST / Resolution: 2.4→38.8 Å
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Refine LS restraints |
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LS refinement shell |
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Refinement TLS params. | Method: refined / Origin x: 99.9705 Å / Origin y: 102.0444 Å / Origin z: 131.1448 Å
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Refinement TLS group | Selection details: all |