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Yorodumi- PDB-5k24: Crystal structure of the complex between phosphatase PRL-2 in the... -
+Open data
-Basic information
Entry | Database: PDB / ID: 5k24 | ||||||
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Title | Crystal structure of the complex between phosphatase PRL-2 in the oxidized state with the Bateman domain of murine magnesium transporter CNNM3 | ||||||
Components |
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Keywords | HYDROLASE/TRANSPORTER PROTEIN/PROTEIN BINDING / Complex / phosphatase / magnesium transporter / protein binding / HYDROLASE / HYDROLASE-TRANSPORTER PROTEIN-PROTEIN BINDING complex | ||||||
Function / homology | Function and homology information RAB geranylgeranylation / transmembrane transporter activity / monoatomic ion transport / protein-tyrosine-phosphatase / protein tyrosine phosphatase activity / membrane => GO:0016020 / early endosome / intracellular membrane-bounded organelle / nucleus / plasma membrane / cytoplasm Similarity search - Function | ||||||
Biological species | Mus musculus (house mouse) | ||||||
Method | X-RAY DIFFRACTION / SYNCHROTRON / Resolution: 3.1 Å | ||||||
Authors | Kozlov, G. / Gulerez, I. / Gehring, K. | ||||||
Citation | Journal: Sci Rep / Year: 2017 Title: PRL3 phosphatase active site is required for binding the putative magnesium transporter CNNM3. Authors: Zhang, H. / Kozlov, G. / Li, X. / Wu, H. / Gulerez, I. / Gehring, K. | ||||||
History |
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-Structure visualization
Structure viewer | Molecule: MolmilJmol/JSmol |
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-Downloads & links
-Download
PDBx/mmCIF format | 5k24.cif.gz | 244.1 KB | Display | PDBx/mmCIF format |
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PDB format | pdb5k24.ent.gz | 208.5 KB | Display | PDB format |
PDBx/mmJSON format | 5k24.json.gz | Tree view | PDBx/mmJSON format | |
Others | Other downloads |
-Validation report
Arichive directory | https://data.pdbj.org/pub/pdb/validation_reports/k2/5k24 ftp://data.pdbj.org/pub/pdb/validation_reports/k2/5k24 | HTTPS FTP |
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-Related structure data
-Links
-Assembly
Deposited unit |
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Unit cell |
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Components on special symmetry positions |
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Noncrystallographic symmetry (NCS) | NCS domain:
NCS domain segments:
NCS ensembles :
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-Components
#1: Protein | Mass: 20893.055 Da / Num. of mol.: 2 Source method: isolated from a genetically manipulated source Source: (gene. exp.) Mus musculus (house mouse) / Gene: Ptp4a2, Prl2 / Production host: Escherichia coli (E. coli) / Strain (production host): BL21 / References: UniProt: O70274, protein-tyrosine-phosphatase #2: Protein | Mass: 17341.756 Da / Num. of mol.: 2 Source method: isolated from a genetically manipulated source Source: (gene. exp.) Mus musculus (house mouse) / Gene: Cnnm3, Acdp3 / Production host: Escherichia coli (E. coli) / Strain (production host): BL21 / References: UniProt: Q32NY4 #3: Chemical | ChemComp-FLC / | |
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-Experimental details
-Experiment
Experiment | Method: X-RAY DIFFRACTION / Number of used crystals: 1 |
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-Sample preparation
Crystal | Density Matthews: 2.6 Å3/Da / Density % sol: 52.65 % |
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Crystal grow | Temperature: 293 K / Method: vapor diffusion, hanging drop / pH: 7 Details: 0.2 M ammonium citrate, 19% PEG 3350, 10 mM L-cysteine |
-Data collection
Diffraction | Mean temperature: 100 K |
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Diffraction source | Source: SYNCHROTRON / Site: CHESS / Beamline: F1 / Wavelength: 0.9773 Å |
Detector | Type: ADSC QUANTUM 270 / Detector: CCD / Date: Nov 20, 2014 |
Radiation | Monochromator: Si 111 channel / Protocol: SINGLE WAVELENGTH / Monochromatic (M) / Laue (L): M / Scattering type: x-ray |
Radiation wavelength | Wavelength: 0.9773 Å / Relative weight: 1 |
Reflection | Resolution: 3.1→50 Å / Num. obs: 13689 / % possible obs: 98.88 % / Observed criterion σ(I): -3 / Redundancy: 4.6 % / Rmerge(I) obs: 0.078 / Net I/σ(I): 33.9 |
Reflection shell | Resolution: 3.1→3.15 Å / Redundancy: 4.7 % / Rmerge(I) obs: 0.644 / Mean I/σ(I) obs: 2.8 / % possible all: 100 |
-Processing
Software |
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Refinement | Resolution: 3.1→50 Å / Cor.coef. Fo:Fc: 0.93 / Cor.coef. Fo:Fc free: 0.881 / SU B: 64.728 / SU ML: 0.517 / Cross valid method: THROUGHOUT / ESU R Free: 0.552
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Solvent computation | Ion probe radii: 0.8 Å / Shrinkage radii: 0.8 Å / VDW probe radii: 1.2 Å | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Displacement parameters | Biso mean: 121.88 Å2
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Refinement step | Cycle: 1 / Resolution: 3.1→50 Å
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Refine LS restraints |
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