PDB-5j5p: AMP-PNP-stabilized ATPase domain of topoisomerase IV from Strepto...

ID or keywords:

Entry

Database: PDB / ID: 5j5p

Title

AMP-PNP-stabilized ATPase domain of topoisomerase IV from Streptococcus pneumoniae, complex type I

Components

DNA (5'-D(*GP*CP*GP*CP*GP*C)-3')
DNA topoisomerase 4 subunit BTopoisomerase

Keywords

ISOMERASE/DNA /

Streptococcus PNEUMONIAE /

TOPOISOMERASE IV /

DNA BINDING /

ISOMERASE / ISOMERASE-DNA COMPLEX /

ATPase domain / T-segment

Function / homology

Function and homology information

DNA topoisomerase type II (double strand cut, ATP-hydrolyzing) activity /

DNA topoisomerase (ATP-hydrolysing) / DNA topological change /

chromosome segregation /

chromosome /

DNA binding /

ATP binding /

metal ion binding
Similarity search - Function

Biological species

Streptococcus pneumoniae (bacteria)
synthetic construct (others)

Method

X-RAY DIFFRACTION /

SYNCHROTRON /

MOLECULAR REPLACEMENT / Resolution: 1.97 Å

Authors

Laponogov, I. / Pan, X.-S. / Skamrova, G. / Umrekar, T. / Fisher, L.M. / Sanderson, M.R.

Funding support

United Kingdom, 1items

Citation

Journal: Nat Commun / Year: 2018
Title: Trapping of the transport-segment DNA by the ATPase domains of a type II topoisomerase.
Authors: Laponogov, I. / Pan, X.S. / Veselkov, D.A. / Skamrova, G.B. / Umrekar, T.R. / Fisher, L.M. / Sanderson, M.R.

History

Deposition	Apr 3, 2016	Deposition site: RCSB / Processing site: PDBE
Revision 1.0	Jul 26, 2017	Provider: repository / Type: Initial release
Revision 1.1	Jul 18, 2018	Group: Data collection / Database references / Category: citation / citation_author Item: _citation.country / _citation.journal_abbrev ..._citation.country / _citation.journal_abbrev / _citation.journal_id_CSD / _citation.journal_id_ISSN / _citation.journal_volume / _citation.page_first / _citation.page_last / _citation.pdbx_database_id_DOI / _citation.pdbx_database_id_PubMed / _citation.title / _citation.year
Revision 1.2	Jan 10, 2024	Group: Data collection / Database references ...Data collection / Database references / Derived calculations / Refinement description Category: chem_comp_atom / chem_comp_bond ...chem_comp_atom / chem_comp_bond / database_2 / pdbx_initial_refinement_model / pdbx_struct_conn_angle / struct_conn Item: _database_2.pdbx_DOI / _database_2.pdbx_database_accession ..._database_2.pdbx_DOI / _database_2.pdbx_database_accession / _pdbx_struct_conn_angle.ptnr1_auth_asym_id / _pdbx_struct_conn_angle.ptnr1_auth_comp_id / _pdbx_struct_conn_angle.ptnr1_auth_seq_id / _pdbx_struct_conn_angle.ptnr1_label_asym_id / _pdbx_struct_conn_angle.ptnr1_label_atom_id / _pdbx_struct_conn_angle.ptnr1_label_comp_id / _pdbx_struct_conn_angle.ptnr1_label_seq_id / _pdbx_struct_conn_angle.ptnr2_auth_asym_id / _pdbx_struct_conn_angle.ptnr2_auth_comp_id / _pdbx_struct_conn_angle.ptnr2_auth_seq_id / _pdbx_struct_conn_angle.ptnr2_label_asym_id / _pdbx_struct_conn_angle.ptnr2_label_atom_id / _pdbx_struct_conn_angle.ptnr2_label_comp_id / _pdbx_struct_conn_angle.ptnr3_auth_asym_id / _pdbx_struct_conn_angle.ptnr3_auth_comp_id / _pdbx_struct_conn_angle.ptnr3_auth_seq_id / _pdbx_struct_conn_angle.ptnr3_label_asym_id / _pdbx_struct_conn_angle.ptnr3_label_atom_id / _pdbx_struct_conn_angle.ptnr3_label_comp_id / _pdbx_struct_conn_angle.ptnr3_label_seq_id / _pdbx_struct_conn_angle.value / _struct_conn.pdbx_dist_value / _struct_conn.ptnr1_auth_asym_id / _struct_conn.ptnr1_auth_comp_id / _struct_conn.ptnr1_auth_seq_id / _struct_conn.ptnr1_label_asym_id / _struct_conn.ptnr1_label_atom_id / _struct_conn.ptnr1_label_comp_id / _struct_conn.ptnr1_label_seq_id / _struct_conn.ptnr2_auth_asym_id / _struct_conn.ptnr2_auth_comp_id / _struct_conn.ptnr2_auth_seq_id / _struct_conn.ptnr2_label_asym_id / _struct_conn.ptnr2_label_atom_id / _struct_conn.ptnr2_label_comp_id

Structure viewer	Molecule: MolmilJmol/JSmol

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Download

PDBx/mmCIF format	5j5p.cif.gz	367 KB	Display	PDBx/mmCIF format
PDB format	pdb5j5p.ent.gz	294.1 KB	Display	PDB format
PDBx/mmJSON format	5j5p.json.gz		Tree view	PDBx/mmJSON format
Others	Other downloads

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Validation report

Arichive directory	https://data.pdbj.org/pub/pdb/validation_reports/j5/5j5p ftp://data.pdbj.org/pub/pdb/validation_reports/j5/5j5p	HTTPS FTP

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Related structure data

Related structure data	5j5qC 1ei1S S: Starting model for refinement C: citing same article (ref.)
Similar structure data	5j5q-1 3zkb-4 3zkb-3 1s16-d 4pbq-d 3oza-1 3zkb-5 3zkb-8 3zkb-2 4v4d-6 Search similar-shape structures by Omokage search (details) Similarity search - Function & homologyF&H Search Similarity search - FunctionF&H Search Similarity search - HomologyF&H Search

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Links

PDB pages	PDBj / wwPDB / NCBI
Related items in Molecule of the Month	#73 - Jan 2006 Topoisomerase similarity (15) #131 - Nov 2010 Inteins similarity (2) #168 - Dec 2013 DNA Helicase similarity (3) #108 - Dec 2008 Hsp90 similarity (1) #182 - Feb 2015 Insulin receptor similarity (1) #214 - Oct 2017 Chimeric Antigen Receptors similarity (1) #238 - Oct 2019 Ribonucleotide Reductase similarity (1) #43 - Jul 2003 Src Tyrosine Kinase similarity (1) #50 - Feb 2004 Glycolytic Enzymes similarity (1) #60 - Dec 2004 Ubiquitin similarity (1) #80 - Aug 2006 AAA+ Proteases similarity (1) #95 - Nov 2007 Multidrug Resistance Transporters similarity (1)

PDB pages

PDBj /

wwPDB /

NCBI

Related items in Molecule of the Month

#73 - Jan 2006
Topoisomerase
similarity (15)
#131 - Nov 2010
Inteins
similarity (2)
#168 - Dec 2013
DNA Helicase
similarity (3)
#108 - Dec 2008
Hsp90
similarity (1)
#182 - Feb 2015
Insulin receptor
similarity (1)
#214 - Oct 2017
Chimeric Antigen Receptors
similarity (1)
#238 - Oct 2019
Ribonucleotide Reductase
similarity (1)
#43 - Jul 2003
Src Tyrosine Kinase
similarity (1)
#50 - Feb 2004
Glycolytic Enzymes
similarity (1)
#60 - Dec 2004
Ubiquitin
similarity (1)
#80 - Aug 2006
AAA+ Proteases
similarity (1)
#95 - Nov 2007
Multidrug Resistance Transporters
similarity (1)

Deposited unit

A: DNA topoisomerase 4 subunit B

B: DNA topoisomerase 4 subunit B

C: DNA (5'-D(*GP*CP*GP*CP*GP*C)-3')

D: DNA (5'-D(*GP*CP*GP*CP*GP*C)-3')

E: DNA (5'-D(*GP*CP*GP*CP*GP*C)-3')

F: DNA (5'-D(*GP*CP*GP*CP*GP*C)-3')

hetero molecules

98.5 kDa, 6 polymers
Search similar-shape structures of this assembly by Omokage search (details)

1

Idetical with deposited unit
defined by author&software

Unit cell

Length a, b, c (Å)	134.440, 136.460, 136.270
Angle α, β, γ (deg.)	90.00, 90.00, 90.00
Int Tables number	20
Space group name H-M	C222₁

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Protein / DNA chain , 2 types, 6 molecules ABCDEF

#1: Protein	DNA topoisomerase 4 subunit B / Topoisomerase / Topoisomerase IV subunit B Mass: 45032.457 Da / Num. of mol.: 2 / Fragment: ATPase N-terminal domain, residues 1-402 Source method: isolated from a genetically manipulated source Source: (gene. exp.) Streptococcus pneumoniae (bacteria) / Gene: parE, SP_0852 / Production host: Escherichia coli BL21(DE3) (bacteria) / References: UniProt: Q59961, EC: 5.99.1.3
#2: DNA chain	DNA (5'-D(GPCPGPCPGPC)-3') Mass: 1810.205 Da / Num. of mol.: 4 Source method: isolated from a genetically manipulated source Source: (gene. exp.) synthetic construct (others) / Production host: synthetic construct (others)

#1: Protein

DNA topoisomerase 4 subunit B /

Topoisomerase / Topoisomerase IV subunit B

Mass: 45032.457 Da / Num. of mol.: 2 / Fragment: ATPase N-terminal domain, residues 1-402
Source method: isolated from a genetically manipulated source
Source: (gene. exp.)

Streptococcus pneumoniae (bacteria) / Gene: parE, SP_0852 / Production host:

Escherichia coli BL21(DE3) (bacteria) / References: UniProt: Q59961, EC: 5.99.1.3

#2: DNA chain

DNA (5'-D(*GP*CP*GP*CP*GP*C)-3')

Mass: 1810.205 Da / Num. of mol.: 4
Source method: isolated from a genetically manipulated source
Source: (gene. exp.) synthetic construct (others) / Production host: synthetic construct (others)

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Non-polymers , 4 types, 832 molecules

#3: Chemical	ChemComp-ANP / PHOSPHOAMINOPHOSPHONIC ACID-ADENYLATE ESTER Mass: 506.196 Da / Num. of mol.: 2 / Source method: obtained synthetically / Formula: C₁₀H₁₇N₆O₁₂P₃ / Comment: AMP-PNP, energy-carrying molecule analogue*YM
#4: Chemical	ChemComp-NA / SODIUM ION Mass: 22.990 Da / Num. of mol.: 2 / Source method: obtained synthetically / Formula: Na
#5: Chemical	ChemComp-MG / MAGNESIUM ION Mass: 24.305 Da / Num. of mol.: 4 / Source method: obtained synthetically / Formula: Mg
#6: Water	ChemComp-HOH / water / Water Mass: 18.015 Da / Num. of mol.: 824 / Source method: isolated from a natural source / Formula: H₂O

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Experiment

Experiment	Method: X-RAY DIFFRACTION / Number of used crystals: 1

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Sample preparation

Crystal	Density Matthews: 3.21 Å³/Da / Density % sol: 61.7 %
Crystal grow	Temperature: 297 K / Method: vapor diffusion, sitting drop / pH: 8.5 Details: 0.1 M KCl, 10 mM MgCl2, 50 mM Tris-Cl, 30% PEG 400. Temp details: Incubator

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Data collection

Diffraction	Mean temperature: 100 K
Diffraction source	Source: SYNCHROTRON / Site: Diamond / Beamline: I02 / Wavelength: 0.91915 Å
Detector	Type: DECTRIS PILATUS 6M-F / Detector: PIXEL / Date: Oct 11, 2015
Radiation	Protocol: SINGLE WAVELENGTH / Monochromatic (M) / Laue (L): M / Scattering type: x-ray
Radiation wavelength	Wavelength: 0.91915 Å / Relative weight: 1
Reflection	Resolution: 1.97→45.177 Å / Num. obs: 88222 / % possible obs: 99.79 % / Redundancy: 6.68 % / CC_1/2: 0.9904 / R_merge(I) obs: 0.063 / Net I/σ(I): 6.4
Reflection shell	Resolution: 1.97→2.04 Å / Redundancy: 7 % / R_merge(I) obs: 0.367 / Mean I/σ(I) obs: 1.7 / % possible all: 99.6

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Processing

Software

Name	Version	Classification
PHENIX	(1.10.1_2155: ???)	refinement
xia2		data reduction
xia2		data scaling
PHASER		phasing

Refinement

Method to determine structure:

MOLECULAR REPLACEMENT
Starting model: derived from 1EI1

1ei1

Resolution: 1.97→45.177 Å / SU ML: 0.14 / Cross valid method: THROUGHOUT / σ(F): 1.35 / Phase error: 16.16

	R_factor	Num. reflection	% reflection
R_free	0.174	4405	4.99 %
R_work	0.1505	-	-
obs	0.1516	88222	99.79 %

Solvent computation

Shrinkage radii: 0.9 Å / VDW probe radii: 1.11 Å

Refinement step

Cycle: LAST / Resolution: 1.97→45.177 Å

	Protein	Nucleic acid	Ligand	Solvent	Total
Num. atoms	6005	480	68	824	7377

Refine LS restraints

Refine-ID	Type	Dev ideal	Number
X-RAY DIFFRACTION	f_bond_d	0.017	6771
X-RAY DIFFRACTION	f_angle_d	1.211	9294
X-RAY DIFFRACTION	f_dihedral_angle_d	24.361	2470
X-RAY DIFFRACTION	f_chiral_restr	0.415	1059
X-RAY DIFFRACTION	f_plane_restr	0.007	1123

LS refinement shell

Resolution (Å)	R_factor R_free	Num. reflection R_free	R_factor R_work	Num. reflection R_work	Refine-ID	% reflection obs (%)
1.97-1.9924	0.2166	152	0.1926	2783	X-RAY DIFFRACTION	100
1.9924-2.0158	0.1906	154	0.1783	2702	X-RAY DIFFRACTION	100
2.0158-2.0404	0.1834	152	0.1663	2798	X-RAY DIFFRACTION	100
2.0404-2.0662	0.2002	161	0.1624	2735	X-RAY DIFFRACTION	100
2.0662-2.0934	0.2055	161	0.1677	2767	X-RAY DIFFRACTION	100
2.0934-2.1221	0.1984	158	0.1629	2736	X-RAY DIFFRACTION	100
2.1221-2.1524	0.187	143	0.1658	2763	X-RAY DIFFRACTION	100
2.1524-2.1846	0.1845	141	0.1605	2782	X-RAY DIFFRACTION	100
2.1846-2.2187	0.1651	114	0.159	2812	X-RAY DIFFRACTION	100
2.2187-2.2551	0.1988	128	0.1509	2786	X-RAY DIFFRACTION	100
2.2551-2.2939	0.1653	143	0.1509	2755	X-RAY DIFFRACTION	100
2.2939-2.3357	0.2042	124	0.152	2825	X-RAY DIFFRACTION	100
2.3357-2.3806	0.1903	156	0.1521	2770	X-RAY DIFFRACTION	100
2.3806-2.4292	0.1747	150	0.1514	2762	X-RAY DIFFRACTION	100
2.4292-2.482	0.1891	137	0.1458	2787	X-RAY DIFFRACTION	100
2.482-2.5397	0.1944	170	0.1501	2764	X-RAY DIFFRACTION	100
2.5397-2.6032	0.1725	148	0.151	2793	X-RAY DIFFRACTION	100
2.6032-2.6736	0.1786	148	0.16	2790	X-RAY DIFFRACTION	100
2.6736-2.7523	0.1875	142	0.1574	2765	X-RAY DIFFRACTION	100
2.7523-2.8411	0.1839	147	0.1535	2796	X-RAY DIFFRACTION	100
2.8411-2.9426	0.1855	168	0.1577	2753	X-RAY DIFFRACTION	100
2.9426-3.0604	0.1893	153	0.1669	2815	X-RAY DIFFRACTION	100
3.0604-3.1996	0.1649	146	0.1604	2808	X-RAY DIFFRACTION	100
3.1996-3.3683	0.1836	144	0.1591	2780	X-RAY DIFFRACTION	100
3.3683-3.5792	0.1651	121	0.1441	2847	X-RAY DIFFRACTION	100
3.5792-3.8555	0.1513	167	0.1353	2823	X-RAY DIFFRACTION	100
3.8555-4.2432	0.1475	126	0.1188	2841	X-RAY DIFFRACTION	100
4.2432-4.8566	0.1076	138	0.1208	2845	X-RAY DIFFRACTION	100
4.8566-6.1164	0.1568	139	0.1502	2881	X-RAY DIFFRACTION	100
6.1164-45.1886	0.2025	174	0.1647	2953	X-RAY DIFFRACTION	99

Refinement TLS params.

Method: refined / Refine-ID: X-RAY DIFFRACTION

ID	L11 (°²)	L12 (°²)	L13 (°²)	L22 (°²)	L23 (°²)	L33 (°²)	S11 (Å °)	S12 (Å °)	S13 (Å °)	S21 (Å °)	S22 (Å °)	S23 (Å °)	S31 (Å °)	S32 (Å °)	S33 (Å °)	T11 (Å²)	T12 (Å²)	T13 (Å²)	T22 (Å²)	T23 (Å²)	T33 (Å²)	Origin x (Å)	Origin y (Å)	Origin z (Å)
1	0.9751	0.1177	1.1593	0.135	0.5247	2.4576	-0.0183	-0.1098	0.0082	0.0118	0.0225	0.0773	0.0293	-0.3457	0.0022	0.1586	0.0151	0.0268	0.18	0.0353	0.1886	29.6902	28.88	-13.1403
2	2.2511	-0.1528	0.2629	2.6443	-0.4817	1.5292	0.0577	-0.2843	0.563	0.1771	-0.0916	0.4721	-0.3688	-0.0348	0.0377	0.2181	0.07	0.0031	0.1207	-0.047	0.2792	38.2645	41.4453	3.1692
3	1.9632	-0.359	0.1254	1.1763	-0.2798	2.2698	-0.046	-0.166	0.3041	0.1401	0.0451	0.0456	-0.2782	-0.2242	-0.012	0.1586	0.0548	0.0004	0.1404	-0.0212	0.1698	33.2253	39.5395	-0.6356
4	8.3986	4.023	-4.2438	4.6215	-2.111	6.0345	0.0863	-0.087	0.1247	0.013	0.0064	-0.0346	-0.0357	0.3309	-0.0855	0.1211	0.0665	-0.008	0.1214	0.0148	0.1081	48.7498	28.6872	7.3699
5	3.3892	-4.3386	-1.8687	6.6606	1.5011	1.837	-0.3257	-0.5645	0.0361	0.7465	0.351	-0.1165	0.5749	1.0507	-0.0484	0.2724	0.1254	-0.002	0.4358	-0.0025	0.2118	64.963	25.8413	1.5987
6	5.9874	-2.6568	-2.1731	1.5976	1.1492	0.7513	-0.2234	0.0567	-0.1248	0.0182	0.1449	0.0271	0.0247	0.0994	0.0516	0.1455	-0.001	-0.0112	0.2506	-0.0103	0.1558	67.0136	24.6634	-14.5679
7	7.2887	-3.2361	2.5116	4.2062	-1.9516	4.1303	-0.0427	0.3203	-0.0223	-0.1087	-0.0197	0.1173	-0.1333	0.1891	0.059	0.1463	0.0053	0.0435	0.1559	-0.0089	0.112	51.833	33.2402	-14.7839
8	7.1886	-5.1454	4.1401	4.8394	-3.2343	4.0907	0.0326	0.8219	0.5821	0.0071	-0.0714	-0.3615	-0.1953	0.2385	0.0021	0.1697	-0.0367	0.0409	0.3297	0.0335	0.1987	70.9183	32.8267	-16.5336
9	4.7757	5.5959	3.9798	9.6994	6.2944	6.4343	0.0965	-0.1078	-0.5046	0.2803	-0.0868	-0.3385	0.5316	-0.1592	-0.0039	0.171	-0.0291	0.0361	0.2727	-0.0095	0.2436	73.7422	8.5522	-16.161
10	1.6359	1.8497	0.1336	6.8881	4.5661	5.1757	-0.4715	0.052	0.0144	0.4201	0.2685	0.2546	0.1277	0.2316	0.1554	0.1508	0.0403	0.0237	0.1245	0.0359	0.1836	39.3522	41.1369	-6.7566
11	0.7307	0.402	0.8151	1.4253	1.1072	2.3816	-0.0505	0.0332	0.1822	0.0152	0.0082	-0.0178	-0.2905	0.0801	0.0363	0.1722	0.0121	0.0119	0.165	0.0491	0.2014	37.7915	36.6952	-22.6032
12	2.9086	-0.5014	-0.5382	2.0769	0.1038	1.5459	-0.0385	0.1417	0.0653	-0.0087	0.0421	0.0723	-0.0361	-0.2035	-0.0017	0.1207	0.032	-0.0252	0.1826	0.0565	0.125	25.7107	27.6249	-39.018
13	0.9921	-0.1392	0.4678	1.1214	0.1537	2.0103	-0.0729	0.0593	0.0592	-0.0981	0.0603	0.0817	-0.1561	-0.1417	0.0152	0.1271	0.0243	0.0162	0.1436	0.0406	0.1451	27.4621	32.8872	-35.3458
14	5.6915	2.9465	-2.3587	7.7204	-2.5344	6.3343	-0.0555	0.0972	-0.0706	-0.1018	0.0734	0.0802	0.4146	-0.0779	-0.019	0.1183	0.0647	0.0231	0.1334	0.0235	0.1193	38.6	17.1155	-42.6592
15	7.3887	-4.1761	1.0012	3.3015	-1.6212	1.3879	0.3668	0.7454	-0.0773	-0.5519	-0.2564	0.0231	1.3516	0.6974	-0.1271	0.5284	0.1707	0.0251	0.348	0.006	0.2288	41.6174	0.8096	-36.1492
16	1.6362	-2.8032	1.4681	6.0051	-3.1914	1.2326	0.0963	0.0386	-0.0041	-0.0346	-0.1497	-0.1118	0.1495	0.0043	0.0411	0.2559	0.0131	-0.0056	0.1644	-0.0051	0.1657	41.4984	-0.5766	-19.524
17	3.0909	-3.2664	-0.8597	6.8199	1.5981	3.0449	-0.0894	-0.099	0.0143	0.2435	0.0107	-0.1482	0.1918	0.0194	0.0814	0.1676	0.0189	-0.0054	0.152	0.057	0.1169	33.3392	14.6526	-20.346
18	5.5441	-4.9086	-2.9721	4.2798	2.9698	2.7278	-0.067	-0.0916	-0.4313	0.4853	0.1997	0.7577	0.0397	-0.1606	-0.1385	0.3512	0.0021	0.0012	0.182	0.0643	0.2515	33.6066	-4.132	-17.7984
19	9.5967	5.1517	6.4909	4.7012	3.947	7.5485	0.0723	0.0376	-0.2391	-0.0004	0.012	-0.3937	-0.0773	0.3229	-0.1118	0.2354	-0.0083	0.0021	0.152	0.0134	0.2357	57.4331	-7.3838	-17.244
20	3.1696	1.7145	3.2594	1.4165	1.8826	3.5693	0.0953	0.1577	0.0307	0.1668	-0.2498	0.1285	0.1924	-0.0443	0.1357	0.1137	0.0099	0.0307	0.1887	0.0691	0.2105	25.7322	26.8426	-29.0358
21	2.2767	3.2424	-0.9603	5.0832	-3.2118	8.4942	-0.0575	-0.5095	0.6335	-0.5628	-0.3851	0.2545	-0.8231	0.7859	0.5293	0.7745	-0.0733	0.0182	0.3712	-0.0109	0.5701	45.293	7.119	-7.7122
22	3.0214	1.1693	-3.3782	9.7248	2.4685	9.317	-1.0416	-0.2863	0.0442	0.2334	0.1721	0.4261	-0.5676	0.6241	0.837	0.8146	0.0309	-0.0354	0.5285	0.0411	0.5077	44.9936	8.004	-1.0954
23	2.635	1.6149	1.2126	8.6747	1.2333	8.5107	-0.2954	-0.4864	0.9485	-0.3472	-0.381	0.783	0.6614	-0.3554	0.7041	0.3809	-0.0671	-0.0195	0.869	0.0108	0.6978	59.8591	20.7544	-26.6894
24	9.4686	0.9957	1.7984	2.5441	-4.0354	9.147	0.1211	-0.0665	0.6475	0.119	-1.1765	-0.153	0.5877	1.0336	0.9469	0.842	-0.0478	-0.0611	1.1224	0.0496	0.5565	59.1683	20.9328	-33.4301

Refinement TLS group

ID	Refine-ID	Refine TLS-ID	Selection details
1	X-RAY DIFFRACTION	1	chain 'A' and (resid 5 through 57 )
2	X-RAY DIFFRACTION	2	chain 'A' and (resid 58 through 93 )
3	X-RAY DIFFRACTION	3	chain 'A' and (resid 94 through 189 )
4	X-RAY DIFFRACTION	4	chain 'A' and (resid 190 through 222 )
5	X-RAY DIFFRACTION	5	chain 'A' and (resid 223 through 245 )
6	X-RAY DIFFRACTION	6	chain 'A' and (resid 246 through 323 )
7	X-RAY DIFFRACTION	7	chain 'A' and (resid 324 through 349 )
8	X-RAY DIFFRACTION	8	chain 'A' and (resid 350 through 371 )
9	X-RAY DIFFRACTION	9	chain 'A' and (resid 372 through 400 )
10	X-RAY DIFFRACTION	10	chain 'A' and resid 800
11	X-RAY DIFFRACTION	11	chain 'B' and (resid 5 through 57 )
12	X-RAY DIFFRACTION	12	chain 'B' and (resid 58 through 93 )
13	X-RAY DIFFRACTION	13	chain 'B' and (resid 94 through 189 )
14	X-RAY DIFFRACTION	14	chain 'B' and (resid 190 through 222 )
15	X-RAY DIFFRACTION	15	chain 'B' and (resid 223 through 245 )
16	X-RAY DIFFRACTION	16	chain 'B' and (resid 246 through 323 )
17	X-RAY DIFFRACTION	17	chain 'B' and (resid 324 through 349 )
18	X-RAY DIFFRACTION	18	chain 'B' and (resid 350 through 371 )
19	X-RAY DIFFRACTION	19	chain 'B' and (resid 372 through 400 )
20	X-RAY DIFFRACTION	20	chain 'B' and resid 800
21	X-RAY DIFFRACTION	21	chain 'C'
22	X-RAY DIFFRACTION	22	chain 'D'
23	X-RAY DIFFRACTION	23	chain 'E'
24	X-RAY DIFFRACTION	24	chain 'F'

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