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- PDB-5j28: Ki67-PP1g (protein phosphatase 1, gamma isoform) holoenzyme complex -
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Open data
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Basic information
Entry | Database: PDB / ID: 5j28 | |||||||||
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Title | Ki67-PP1g (protein phosphatase 1, gamma isoform) holoenzyme complex | |||||||||
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![]() | HYDROLASE/PROTEIN BINDING / PP1 gamma / RepoMan / Ki-67 / ![]() | |||||||||
Function / homology | ![]() ![]() ![]() ![]() ![]() ![]() ![]() ![]() ![]() ![]() ![]() ![]() ![]() ![]() ![]() ![]() ![]() ![]() ![]() ![]() ![]() ![]() ![]() ![]() ![]() ![]() ![]() ![]() ![]() ![]() ![]() ![]() ![]() ![]() ![]() ![]() Similarity search - Function | |||||||||
Biological species | ![]() ![]() | |||||||||
Method | ![]() ![]() ![]() | |||||||||
![]() | Kumar, G.S. / Peti, W. / Page, R. | |||||||||
Funding support | ![]()
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![]() | ![]() Title: The Ki-67 and RepoMan mitotic phosphatases assemble via an identical, yet novel mechanism. Authors: Kumar, G.S. / Gokhan, E. / De Munter, S. / Bollen, M. / Vagnarelli, P. / Peti, W. / Page, R. | |||||||||
History |
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Structure visualization
Structure viewer | Molecule: ![]() ![]() |
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Downloads & links
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Download
PDBx/mmCIF format | ![]() | 146.4 KB | Display | ![]() |
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PDB format | ![]() | 112.7 KB | Display | ![]() |
PDBx/mmJSON format | ![]() | Tree view | ![]() | |
Others | ![]() |
-Validation report
Arichive directory | ![]() ![]() | HTTPS FTP |
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-Related structure data
Related structure data | ![]() 5inbSC ![]() 5iohC S: Starting model for refinement C: citing same article ( |
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Similar structure data | |
Other databases |
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Links
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Assembly
Deposited unit | ![]()
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1 | ![]()
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2 | ![]()
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Unit cell |
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Components
#1: Protein | Mass: 35044.363 Da / Num. of mol.: 2 / Fragment: UNP residues 7-308 Source method: isolated from a genetically manipulated source Source: (gene. exp.) ![]() ![]() ![]() ![]() ![]() References: UniProt: P36873, protein-serine/threonine phosphatase #2: Protein/peptide | Mass: 5187.017 Da / Num. of mol.: 2 / Fragment: UNP residues 496-536 / Mutation: T525M Source method: isolated from a genetically manipulated source Source: (gene. exp.) ![]() ![]() ![]() ![]() ![]() #3: Chemical | ![]() #4: Chemical | ChemComp-NA / | #5: Water | ChemComp-HOH / | ![]() |
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-Experimental details
-Experiment
Experiment | Method: ![]() |
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Sample preparation
Crystal | Density Matthews: 3.06 Å3/Da / Density % sol: 59.8 % |
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Crystal grow![]() | Temperature: 277 K / Method: vapor diffusion, hanging drop / pH: 4 / Details: 1.9 M Sodium Malonate pH 4.0 |
-Data collection
Diffraction | Mean temperature: 100 K |
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Diffraction source | Source: ![]() ![]() ![]() |
Detector | Type: DECTRIS PILATUS 6M / Detector: PIXEL / Date: Mar 19, 2016 |
Radiation | Monochromator: Liquid nitrogen-cooled double crystal, non fixed exit slit Protocol: SINGLE WAVELENGTH / Monochromatic (M) / Laue (L): M / Scattering type: x-ray |
Radiation wavelength | Wavelength![]() |
Reflection | Resolution: 2→39.331 Å / Num. obs: 64993 / % possible obs: 100 % / Observed criterion σ(I): 3.1 / Redundancy: 10.4 % / Rmerge(I) obs: 0.092 / Net I/σ(I): 20.5 |
Reflection shell | Resolution: 2→2.05 Å / Redundancy: 10.3 % / Rmerge(I) obs: 0.0861 / Mean I/σ(I) obs: 3.1 / % possible all: 92.2 |
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Processing
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Refinement | Method to determine structure![]() ![]() Starting model: 5INB Resolution: 2→39.331 Å / Cross valid method: THROUGHOUT / σ(F): 1.35 / Phase error: 20.23 / Stereochemistry target values: TWIN_LSQ_F
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Solvent computation | Shrinkage radii: 0.9 Å / VDW probe radii: 1.11 Å / Solvent model: FLAT BULK SOLVENT MODEL | |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Refinement step | Cycle: LAST / Resolution: 2→39.331 Å
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Refine LS restraints |
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LS refinement shell |
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