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Yorodumi- PDB-5igc: Crystal structure of Staphylococcal nuclease variant Delta+PHS V2... -
+Open data
-Basic information
Entry | Database: PDB / ID: 5igc | ||||||
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Title | Crystal structure of Staphylococcal nuclease variant Delta+PHS V23E/V66H at cryogenic temperature | ||||||
Components | ThermonucleaseMicrococcal nuclease | ||||||
Keywords | HYDROLASE / nuclease / hyperstable / pdTp / ionizable group | ||||||
Function / homology | Function and homology information endonuclease activity, active with either ribo- or deoxyribonucleic acids and producing 3'-phosphomonoesters / micrococcal nuclease / nucleic acid binding / extracellular region / membrane / metal ion binding Similarity search - Function | ||||||
Biological species | Staphylococcus aureus (bacteria) | ||||||
Method | X-RAY DIFFRACTION / MOLECULAR REPLACEMENT / molecular replacement / Resolution: 1.8 Å | ||||||
Authors | Robinson, A.C. / Theodoru, A. / Schlessman, J.L. / Garcia-Moreno E., B. | ||||||
Funding support | United States, 1items
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Citation | Journal: To be Published Title: Crystal structure of Staphylococcal nuclease variant Delta+PHS V23E/V66H at cryogenic temperature Authors: Robinson, A.C. / Theodoru, A. / Schlessman, J.L. / Garcia-Moreno E., B. | ||||||
History |
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-Structure visualization
Structure viewer | Molecule: MolmilJmol/JSmol |
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-Downloads & links
-Download
PDBx/mmCIF format | 5igc.cif.gz | 46.1 KB | Display | PDBx/mmCIF format |
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PDB format | pdb5igc.ent.gz | 29.5 KB | Display | PDB format |
PDBx/mmJSON format | 5igc.json.gz | Tree view | PDBx/mmJSON format | |
Others | Other downloads |
-Validation report
Arichive directory | https://data.pdbj.org/pub/pdb/validation_reports/ig/5igc ftp://data.pdbj.org/pub/pdb/validation_reports/ig/5igc | HTTPS FTP |
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-Related structure data
Related structure data | 3bdcS S: Starting model for refinement |
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Similar structure data |
-Links
-Assembly
Deposited unit |
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1 |
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Unit cell |
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-Components
#1: Protein | Mass: 16212.465 Da / Num. of mol.: 1 / Fragment: UNP residues 83-231 / Mutation: V23E/G50F/V51N/V66H/P117G/H124L/S128A/Del44-49 Source method: isolated from a genetically manipulated source Source: (gene. exp.) Staphylococcus aureus (bacteria) / Gene: nuc / Plasmid: pET24a+ / Production host: Escherichia coli BL21(DE3) (bacteria) / References: UniProt: P00644, micrococcal nuclease |
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#2: Chemical | ChemComp-CA / |
#3: Chemical | ChemComp-THP / |
#4: Water | ChemComp-HOH / |
-Experimental details
-Experiment
Experiment | Method: X-RAY DIFFRACTION / Number of used crystals: 1 |
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-Sample preparation
Crystal | Density Matthews: 2.23 Å3/Da / Density % sol: 44.96 % |
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Crystal grow | Temperature: 277 K / Method: vapor diffusion, hanging drop / pH: 6 Details: 20% MPD, 25 mM potassium phosphate, calcium chloride, pdTp |
-Data collection
Diffraction | Mean temperature: 110 K |
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Diffraction source | Source: SEALED TUBE / Type: OTHER / Wavelength: 1.54 Å |
Detector | Type: AGILENT ATLAS CCD / Detector: CCD / Date: Sep 22, 2015 |
Radiation | Monochromator: mirrors / Protocol: SINGLE WAVELENGTH / Monochromatic (M) / Laue (L): M / Scattering type: x-ray |
Radiation wavelength | Wavelength: 1.54 Å / Relative weight: 1 |
Reflection | Resolution: 1.8→50 Å / Num. obs: 13318 / % possible obs: 99.9 % / Observed criterion σ(F): 0 / Observed criterion σ(I): 0 / Redundancy: 10.1 % / Biso Wilson estimate: 25.8 Å2 / Rmerge(I) obs: 0.019 / Net I/σ(I): 51.09 |
Reflection shell | Resolution: 1.8→1.87 Å / Redundancy: 6.8 % / Rmerge(I) obs: 0.123 / Mean I/σ(I) obs: 6.24 / % possible all: 100 |
-Phasing
Phasing | Method: molecular replacement | |||||||||
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Phasing MR | Model details: Phaser MODE: MR_AUTO
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-Processing
Software |
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Refinement | Method to determine structure: MOLECULAR REPLACEMENT Starting model: PDB entry 3BDC Resolution: 1.8→38.45 Å / Cor.coef. Fo:Fc: 0.953 / Cor.coef. Fo:Fc free: 0.93 / SU B: 3.018 / SU ML: 0.093 / SU R Cruickshank DPI: 0.1332 / Cross valid method: THROUGHOUT / σ(F): 0 / ESU R: 0.133 / ESU R Free: 0.126 / Details: HYDROGENS HAVE BEEN ADDED IN THE RIDING POSITIONS
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Solvent computation | Ion probe radii: 0.8 Å / Shrinkage radii: 0.8 Å / VDW probe radii: 1.2 Å | |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Displacement parameters | Biso max: 59.69 Å2 / Biso mean: 21.929 Å2 / Biso min: 9.07 Å2
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Refinement step | Cycle: final / Resolution: 1.8→38.45 Å
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Refine LS restraints |
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LS refinement shell | Resolution: 1.8→1.847 Å / Total num. of bins used: 20
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