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Open data
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Basic information
Entry | Database: PDB / ID: 5i1j | ||||||
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Title | CRYSTAL STRUCTURE OF HUMAN GERMLINE ANTIBODY IGHV5-51/IGKV3-11 | ||||||
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Function / homology | ![]() ![]() ![]() ![]() | ||||||
Biological species | ![]() ![]() | ||||||
Method | ![]() ![]() | ||||||
![]() | Teplyakov, A. / Obmolova, G. / Malia, T. / Luo, J. / Gilliland, G. | ||||||
![]() | ![]() Title: Structural diversity in a human antibody germline library. Authors: Teplyakov, A. / Obmolova, G. / Malia, T.J. / Luo, J. / Muzammil, S. / Sweet, R. / Almagro, J.C. / Gilliland, G.L. #1: Journal: Acta Crystallogr F Struct Biol Commun / Year: 2014 Title: Protein crystallization with microseed matrix screening: application to human germline antibody Fabs. Authors: Obmolova, G. / Malia, T.J. / Teplyakov, A. / Sweet, R.W. / Gilliland, G.L. | ||||||
History |
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Structure visualization
Structure viewer | Molecule: ![]() ![]() |
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Downloads & links
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Download
PDBx/mmCIF format | ![]() | 96.5 KB | Display | ![]() |
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PDB format | ![]() | 76 KB | Display | ![]() |
PDBx/mmJSON format | ![]() | Tree view | ![]() | |
Others | ![]() |
-Validation report
Arichive directory | ![]() ![]() | HTTPS FTP |
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-Related structure data
Related structure data | ![]() 5i15C ![]() 5i16C ![]() 5i17C ![]() 5i18C ![]() 5i19C ![]() 5i1aC ![]() 5i1cC ![]() 5i1dC ![]() 5i1eC ![]() 5i1gC ![]() 5i1hC ![]() 5i1iC ![]() 5i1kC ![]() 5i1lC C: citing same article ( |
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Similar structure data |
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Links
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Assembly
Deposited unit | ![]()
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Unit cell |
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Components
#1: Antibody | ![]() Mass: 23421.984 Da / Num. of mol.: 1 Source method: isolated from a genetically manipulated source Source: (gene. exp.) ![]() ![]() ![]() ![]() |
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#2: Antibody | ![]() Mass: 24544.506 Da / Num. of mol.: 1 Source method: isolated from a genetically manipulated source Source: (gene. exp.) ![]() ![]() ![]() ![]() |
#3: Chemical | ChemComp-GOL / ![]() |
#4: Water | ChemComp-HOH / ![]() |
-Experimental details
-Experiment
Experiment | Method: ![]() |
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Sample preparation
Crystal | Density Matthews: 2.56 Å3/Da / Density % sol: 52 % |
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Crystal grow![]() | Temperature: 293 K / Method: vapor diffusion, sitting drop / pH: 8.5 Details: 0.1 M TRIS PH 8.5, 25% PEG 3350, 0.2 M MAGNESIUM CHLORIDE PH range: 8.5 |
-Data collection
Diffraction | Mean temperature: 95 K |
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Diffraction source | Source: ![]() |
Detector | Type: RIGAKU SATURN 944 / Detector: CCD / Date: Apr 22, 2009 / Details: VARIMAX HF |
Radiation | Protocol: SINGLE WAVELENGTH / Monochromatic (M) / Laue (L): M / Scattering type: x-ray |
Radiation wavelength | Wavelength![]() |
Reflection | Resolution: 2.5→30 Å / Num. obs: 17286 / % possible obs: 99.7 % / Observed criterion σ(I): -3 / Redundancy: 7 % / Biso Wilson estimate: 27 Å2 / Rmerge(I) obs: 0.08 / Net I/σ(I): 21.1 |
Reflection shell | Resolution: 2.5→2.57 Å / Redundancy: 6.5 % / Rmerge(I) obs: 0.281 / Mean I/σ(I) obs: 6.9 / % possible all: 97.3 |
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Processing
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Refinement | Method to determine structure![]() ![]()
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Solvent computation | Ion probe radii: 0.8 Å / Shrinkage radii: 0.8 Å / VDW probe radii: 1.4 Å | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Displacement parameters | Biso mean: 38.2 Å2
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Refinement step | Cycle: LAST / Resolution: 2.5→15 Å
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