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Yorodumi- PDB-5hrh: The crystal structure of AsfvPolX(H115F/R127A mutant): 1nt-gap(P)... -
+Open data
-Basic information
Entry | Database: PDB / ID: 5hrh | ||||||
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Title | The crystal structure of AsfvPolX(H115F/R127A mutant): 1nt-gap(P) DNA2:dGTP ternary complex | ||||||
Components |
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Keywords | TRANSFERASE/DNA / ASFV / PolX / TRANSFERASE-DNA complex | ||||||
Function / homology | Function and homology information virion component / base-excision repair / double-strand break repair via nonhomologous end joining / DNA-directed DNA polymerase / DNA-directed DNA polymerase activity / DNA binding / metal ion binding Similarity search - Function | ||||||
Biological species | African swine fever virus synthetic construct (others) | ||||||
Method | X-RAY DIFFRACTION / SYNCHROTRON / Resolution: 3 Å | ||||||
Authors | Chen, Y.Q. / Zhang, J. / Gan, J.H. | ||||||
Citation | Journal: To Be Published Title: The crystal structure of Se-AsfvPolX(L52/163M mutant) in complex with 1nt-gap DNA1 Authors: Chen, Y.Q. / Zhang, J. / Gan, J.H. | ||||||
History |
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-Structure visualization
Structure viewer | Molecule: MolmilJmol/JSmol |
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-Downloads & links
-Download
PDBx/mmCIF format | 5hrh.cif.gz | 233.3 KB | Display | PDBx/mmCIF format |
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PDB format | pdb5hrh.ent.gz | 184.3 KB | Display | PDB format |
PDBx/mmJSON format | 5hrh.json.gz | Tree view | PDBx/mmJSON format | |
Others | Other downloads |
-Validation report
Arichive directory | https://data.pdbj.org/pub/pdb/validation_reports/hr/5hrh ftp://data.pdbj.org/pub/pdb/validation_reports/hr/5hrh | HTTPS FTP |
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-Related structure data
Related structure data | 5hr9C 5hrbC 5hrdC 5hreC 5hrfC 5hrgC 5hrkC 5hrlC C: citing same article (ref.) |
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Similar structure data |
-Links
-Assembly
Deposited unit |
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1 |
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2 |
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Unit cell |
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-Components
-Protein , 1 types, 2 molecules AB
#1: Protein | Mass: 20532.635 Da / Num. of mol.: 2 / Mutation: H115F, R127A Source method: isolated from a genetically manipulated source Source: (gene. exp.) African swine fever virus / Gene: O174L / Production host: Escherichia coli (E. coli) / Strain (production host): BL21(DE3) / References: UniProt: A0A0A1E3N6, UniProt: P42494*PLUS |
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-DNA chain , 3 types, 6 molecules CFDGEH
#2: DNA chain | Mass: 5457.539 Da / Num. of mol.: 2 / Source method: obtained synthetically / Source: (synth.) synthetic construct (others) #3: DNA chain | Mass: 2779.848 Da / Num. of mol.: 2 / Source method: obtained synthetically / Source: (synth.) synthetic construct (others) #4: DNA chain | Mass: 2459.656 Da / Num. of mol.: 2 / Source method: obtained synthetically / Source: (synth.) synthetic construct (others) |
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-Non-polymers , 3 types, 8 molecules
#5: Chemical | #6: Chemical | ChemComp-MN / #7: Water | ChemComp-HOH / | |
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-Experimental details
-Experiment
Experiment | Method: X-RAY DIFFRACTION / Number of used crystals: 1 |
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-Sample preparation
Crystal | Density Matthews: 2.98 Å3/Da / Density % sol: 58.79 % |
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Crystal grow | Temperature: 277 K / Method: vapor diffusion, hanging drop / pH: 7.5 Details: 20% (w/v) PEG 3350, 100 mM HEPES pH 7.5, 200 mM Sodium chloride |
-Data collection
Diffraction | Mean temperature: 100 K |
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Diffraction source | Source: SYNCHROTRON / Site: SSRF / Beamline: BL17U / Wavelength: 0.9793 Å |
Detector | Type: ADSC QUANTUM 315r / Detector: CCD / Date: Jul 20, 2015 |
Radiation | Protocol: SINGLE WAVELENGTH / Monochromatic (M) / Laue (L): M / Scattering type: x-ray |
Radiation wavelength | Wavelength: 0.9793 Å / Relative weight: 1 |
Reflection | Resolution: 3→30 Å / Num. obs: 15302 / % possible obs: 99.1 % / Redundancy: 11 % / Net I/σ(I): 16.7 |
Reflection shell | Resolution: 3→3.18 Å |
-Processing
Software |
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Refinement | Resolution: 3→29.986 Å / SU ML: 0.43 / Cross valid method: FREE R-VALUE / σ(F): 1.34 / Phase error: 37.71 / Stereochemistry target values: ML
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Solvent computation | Shrinkage radii: 0.9 Å / VDW probe radii: 1.11 Å / Solvent model: FLAT BULK SOLVENT MODEL | |||||||||||||||||||||||||||||||||||||||||||||||||
Refinement step | Cycle: LAST / Resolution: 3→29.986 Å
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Refine LS restraints |
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LS refinement shell |
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Refinement TLS params. | Method: refined / Origin x: 151.6932 Å / Origin y: 70.7065 Å / Origin z: 107.6381 Å
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Refinement TLS group | Selection details: all |