+Open data
-Basic information
Entry | Database: PDB / ID: 5gm0 | |||||||||
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Title | Tl-gal with lactose | |||||||||
Components | galectin | |||||||||
Keywords | SUGAR BINDING PROTEIN / Carbohydrates / Toxascaris leonina / Galectin | |||||||||
Function / homology | Function and homology information | |||||||||
Biological species | Toxascaris leonina (invertebrata) | |||||||||
Method | X-RAY DIFFRACTION / SYNCHROTRON / MOLECULAR REPLACEMENT / Resolution: 1.7 Å | |||||||||
Authors | Hwang, E.Y. | |||||||||
Funding support | Korea, Republic Of, 1items
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Citation | Journal: J. Biol. Chem. / Year: 2016 Title: Structural Basis for Carbohydrate Recognition and Anti-inflammatory Modulation by Gastrointestinal Nematode Parasite Toxascaris leonina Galectin Authors: Hwang, E.Y. / Jeong, M.S. / Park, S.K. / Ha, S.C. / Yu, H.S. / Jang, S.B. #1: Journal: Acta Crystallogr.,Sect.D / Year: 2013 Title: Structure of full-length Toxascaris leonina galectin with two carbohydrate-recognition domains Authors: Jeong, M.S. / Hwang, H.G. / Yu, H.S. / Jang, S.B. | |||||||||
History |
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-Structure visualization
Structure viewer | Molecule: MolmilJmol/JSmol |
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-Downloads & links
-Download
PDBx/mmCIF format | 5gm0.cif.gz | 137.4 KB | Display | PDBx/mmCIF format |
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PDB format | pdb5gm0.ent.gz | 104.5 KB | Display | PDB format |
PDBx/mmJSON format | 5gm0.json.gz | Tree view | PDBx/mmJSON format | |
Others | Other downloads |
-Validation report
Arichive directory | https://data.pdbj.org/pub/pdb/validation_reports/gm/5gm0 ftp://data.pdbj.org/pub/pdb/validation_reports/gm/5gm0 | HTTPS FTP |
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-Related structure data
Related structure data | 5gltC 5gluC 5glvC 5glwC 5glzC 4hl0S C: citing same article (ref.) S: Starting model for refinement |
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Similar structure data |
-Links
-Assembly
Deposited unit |
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1 |
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2 |
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Unit cell |
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-Components
#1: Protein | Mass: 32324.168 Da / Num. of mol.: 2 Source method: isolated from a genetically manipulated source Source: (gene. exp.) Toxascaris leonina (invertebrata) / Production host: Escherichia coli (E. coli) / References: UniProt: A0A1L1QJZ7*PLUS #2: Polysaccharide | #3: Water | ChemComp-HOH / | |
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-Experimental details
-Experiment
Experiment | Method: X-RAY DIFFRACTION / Number of used crystals: 1 |
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-Sample preparation
Crystal | Density Matthews: 3.01 Å3/Da / Density % sol: 59.08 % |
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Crystal grow | Temperature: 293 K / Method: vapor diffusion, hanging drop Details: polyethylene glycol 4000, 0.1M Na HEPES pH 7.5, 0.1M ammonium sulfate |
-Data collection
Diffraction | Mean temperature: 100 K |
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Diffraction source | Source: SYNCHROTRON / Site: PAL/PLS / Beamline: 7A (6B, 6C1) / Wavelength: 0.987 Å |
Detector | Type: MAC Science DIP-320 / Detector: IMAGE PLATE / Date: Jun 24, 2014 |
Radiation | Protocol: SINGLE WAVELENGTH / Monochromatic (M) / Laue (L): M / Scattering type: x-ray |
Radiation wavelength | Wavelength: 0.987 Å / Relative weight: 1 |
Reflection | Resolution: 1.7→30 Å / Num. obs: 81697 / % possible obs: 98.7 % / Redundancy: 3.7 % / Rmerge(I) obs: 0.073 / Net I/σ(I): 23.7 |
Reflection shell | Resolution: 1.7→1.76 Å / Rmerge(I) obs: 0.33 |
-Processing
Software |
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Refinement | Method to determine structure: MOLECULAR REPLACEMENT Starting model: 4HL0 Resolution: 1.7→30 Å / Cross valid method: FREE R-VALUE / σ(F): 0
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Solvent computation | Bsol: 64.5014 Å2 | ||||||||||||||||||||
Displacement parameters | Biso max: 80.23 Å2 / Biso mean: 26.6831 Å2 / Biso min: 11 Å2
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Refinement step | Cycle: LAST / Resolution: 1.7→30 Å
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Refine LS restraints |
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Xplor file |
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