Green Fluorescent Protein / Green fluorescent protein / Green fluorescent protein, GFP / Green fluorescent protein-related / Green fluorescent protein / Green fluorescent protein / Beta Barrel / Mainly Beta Similarity search - Domain/homology
A: Green to red photoconvertible GFP-like protein EosFP B: Green to red photoconvertible GFP-like protein EosFP C: Green to red photoconvertible GFP-like protein EosFP D: Green to red photoconvertible GFP-like protein EosFP hetero molecules
Mass: 18.015 Da / Num. of mol.: 344 / Source method: isolated from a natural source / Formula: H2O
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Experimental details
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Experiment
Experiment
Method: X-RAY DIFFRACTION
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Sample preparation
Crystal
Density Matthews: 2.7 Å3/Da / Density % sol: 54.43 % Description: DATA WERE COLLECTED BY SERIAL FEMTOSECOND CRYSTALLOGRAPHY. HENCE THE RMERGE REPORTED ABOVE IS IN FACT THE RSPLIT. 10899 PATTERNS WERE INDEXED.
Crystal grow
Temperature: 293 K / Method: vapor diffusion, hanging drop / pH: 7 Details: 3 M AMMONIUM SULFATE, 100MM BICINE, PH 8.2, VAPOR DIFFUSION, HANGING DROP, TEMPERATURE 293K
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Data collection
Diffraction
Mean temperature: 297 K / Serial crystal experiment: Y
Diffraction source
Source: FREE ELECTRON LASER / Site: SACLA / Beamline: BL3 / Wavelength: 1.77 / Wavelength: 1.77 Å
Detector
Type: MPCCD / Detector: CCD / Date: Feb 14, 2014
Radiation
Protocol: SINGLE WAVELENGTH / Monochromatic (M) / Laue (L): M / Scattering type: x-ray
Radiation wavelength
Wavelength: 1.77 Å / Relative weight: 1
Reflection
Resolution: 2.75→50 Å / Num. obs: 30981 / % possible obs: 100 % / Observed criterion σ(I): 0.95 / Redundancy: 159 % / Rmerge(I) obs: 0.22 / Net I/σ(I): 3.39
Reflection shell
Resolution: 2.75→2.8 Å / Redundancy: 70 % / Rmerge(I) obs: 1 / Mean I/σ(I) obs: 0.95 / % possible all: 100