Green Fluorescent Protein / Green fluorescent protein / Green fluorescent protein-related / Green fluorescent protein / Green fluorescent protein / bioluminescence / Beta Barrel / Mainly Beta / GFP-like fluorescent chromoprotein FP538
Mass: 18.015 Da / Num. of mol.: 56 / Source method: isolated from a natural source / Formula: H2O
Sequence details
Residue LYS 66, TYR 67 and GLY 68 are modified to make chromophore (CH7 66). ZsYellow chromophore ...Residue LYS 66, TYR 67 and GLY 68 are modified to make chromophore (CH7 66). ZsYellow chromophore has a three-ring feature by hetero-cyclization of the Lys66 residue, resulting in backbone cleavage between Phe65 and Lys66.
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Experimental details
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Experiment
Experiment
Method: X-RAY DIFFRACTION / Number of used crystals: 1
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Sample preparation
Crystal
Density Matthews: 2.12 Å3/Da / Density % sol: 42 %
Crystal grow
Temperature: 295.5 K / Method: vapor diffusion, hanging drop / Details: Tris
Resolution: 2.9→19.91 Å / Cor.coef. Fo:Fc: 0.937 / Cor.coef. Fo:Fc free: 0.882 / SU B: 13.707 / SU ML: 0.265 / Cross valid method: THROUGHOUT / ESU R Free: 0.46 / Stereochemistry target values: MAXIMUM LIKELIHOOD / Details: HYDROGENS HAVE BEEN ADDED IN THE RIDING POSITIONS
Rfactor
Num. reflection
% reflection
Selection details
Rfree
0.23613
455
4.9 %
RANDOM
Rwork
0.17443
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obs
0.1774
8909
91.68 %
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Solvent computation
Ion probe radii: 0.8 Å / Shrinkage radii: 0.8 Å / VDW probe radii: 1.2 Å / Solvent model: MASK