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Open data
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Basic information
Entry | Database: PDB / ID: 5cu6 | ||||||
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Title | Crystal Structure of CK2alpha | ||||||
![]() | Casein kinase II subunit alpha![]() | ||||||
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Function / homology | ![]() regulation of chromosome separation / positive regulation of aggrephagy / WNT mediated activation of DVL / Condensation of Prometaphase Chromosomes / ![]() ![]() ![]() ![]() ![]() ![]() ![]() ![]() ![]() ![]() ![]() ![]() ![]() ![]() ![]() Similarity search - Function | ||||||
Biological species | ![]() ![]() | ||||||
Method | ![]() ![]() ![]() ![]() | ||||||
![]() | Brear, P. / De Fusco, C. / Georgiou, K.H. / Spring, D. / Hyvonen, M. | ||||||
Funding support | ![]()
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![]() | ![]() Title: Specific inhibition of CK2 alpha from an anchor outside the active site. Authors: Brear, P. / De Fusco, C. / Hadje Georgiou, K. / Francis-Newton, N.J. / Stubbs, C.J. / Sore, H.F. / Venkitaraman, A.R. / Abell, C. / Spring, D.R. / Hyvonen, M. | ||||||
History |
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Structure visualization
Structure viewer | Molecule: ![]() ![]() |
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Downloads & links
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Download
PDBx/mmCIF format | ![]() | 92 KB | Display | ![]() |
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PDB format | ![]() | 67.6 KB | Display | ![]() |
PDBx/mmJSON format | ![]() | Tree view | ![]() | |
Others | ![]() |
-Validation report
Arichive directory | ![]() ![]() | HTTPS FTP |
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-Related structure data
Related structure data | ![]() 5clpC ![]() 5cs6C ![]() 5cshC ![]() 5cspC ![]() 5csvC ![]() 5cu3C ![]() 5cu4C ![]() 5cvfC ![]() 5cvgC ![]() 5cvhC ![]() 3warS S: Starting model for refinement C: citing same article ( |
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Similar structure data |
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Links
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Assembly
Deposited unit | ![]()
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Unit cell |
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Components
#1: Protein | ![]() Mass: 39031.391 Da / Num. of mol.: 1 / Fragment: residues 2-329 / Mutation: R21S, K74A, K75A, K76A Source method: isolated from a genetically manipulated source Source: (gene. exp.) ![]() ![]() ![]() ![]() ![]() References: UniProt: P68400, ![]() | ||
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#2: Chemical | ChemComp-ATP / ![]() | ||
#3: Chemical | ![]() #4: Water | ChemComp-HOH / | ![]() |
-Experimental details
-Experiment
Experiment | Method: ![]() |
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Sample preparation
Crystal | Density Matthews: 2.02 Å3/Da / Density % sol: 39.23 % |
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Crystal grow![]() | Temperature: 298 K / Method: vapor diffusion, hanging drop / pH: 6.5 Details: 112.5mM Mes pH 6.5, 35% glycerol ethoxylate, 180 mM ammonium acetate |
-Data collection
Diffraction | Mean temperature: 100 K |
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Diffraction source | Source: ![]() ![]() ![]() |
Detector | Type: DECTRIS PILATUS 6M / Detector: PIXEL / Date: Apr 15, 2015 |
Radiation | Protocol: SINGLE WAVELENGTH / Monochromatic (M) / Laue (L): M / Scattering type: x-ray |
Radiation wavelength | Wavelength![]() |
Reflection | Resolution: 1.36→54.55 Å / Num. obs: 67061 / % possible obs: 99.7 % / Redundancy: 3.3 % / Biso Wilson estimate: 18.17 Å2 / CC1/2: 0.997 / Rmerge(I) obs: 0.055 / Rpim(I) all: 0.036 / Rsym value: 0.055 / Net I/σ(I): 11.8 / Num. measured all: 220382 |
Reflection shell | Resolution: 1.36→1.364 Å / Redundancy: 3 % / Rmerge(I) obs: 0.543 / Mean I/σ(I) obs: 2 / Num. measured all: 50520 / Num. unique all: 15883 / CC1/2: 0.831 / Rpim(I) all: 0.275 / Rsym value: 0.543 / Rejects: 0 / % possible all: 98.3 |
-Phasing
Phasing![]() | Method: ![]() |
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Processing
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Refinement | Method to determine structure![]() ![]() Starting model: 3WAR Resolution: 1.36→54.55 Å / Cor.coef. Fo:Fc: 0.9582 / Cor.coef. Fo:Fc free: 0.9468 / SU R Cruickshank DPI: 0.06 / Cross valid method: THROUGHOUT / σ(F): 0 / SU R Blow DPI: 0.06 / SU Rfree Blow DPI: 0.061 / SU Rfree Cruickshank DPI: 0.061
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Displacement parameters | Biso max: 102.55 Å2 / Biso mean: 24.44 Å2 / Biso min: 8.23 Å2
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Refine analyze | Luzzati coordinate error obs: 0.161 Å | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Refinement step | Cycle: final / Resolution: 1.36→54.55 Å
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Refine LS restraints |
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LS refinement shell | Resolution: 1.35→1.39 Å / Total num. of bins used: 20
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