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- PDB-5aox: Human Alu RNA retrotransposition complex in the ribosome-stalling... -

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Basic information

Entry
Database: PDB / ID: 5aox
TitleHuman Alu RNA retrotransposition complex in the ribosome-stalling conformation
Components
  • (SIGNAL RECOGNITION PARTICLE ...) x 2
  • ALU JO CONSENSUS RNA
KeywordsTRANSLATION / RETROTRANSPOSITION / PROTEIN TARGETING / RNA / MOBILE DNA / SINE / LINE / RIBONUCLEOPROTEIN PARTICLE / SIGNAL RECOGNITION PARTICLE
Function / homology
Function and homology information


signal recognition particle receptor complex / endoplasmic reticulum signal peptide binding / signal recognition particle, endoplasmic reticulum targeting / signal recognition particle binding / negative regulation of translational elongation / cotranslational protein targeting to membrane / protein targeting to ER / 7S RNA binding / SRP-dependent cotranslational protein targeting to membrane / SRP-dependent cotranslational protein targeting to membrane ...signal recognition particle receptor complex / endoplasmic reticulum signal peptide binding / signal recognition particle, endoplasmic reticulum targeting / signal recognition particle binding / negative regulation of translational elongation / cotranslational protein targeting to membrane / protein targeting to ER / 7S RNA binding / SRP-dependent cotranslational protein targeting to membrane / SRP-dependent cotranslational protein targeting to membrane / secretory granule lumen / ficolin-1-rich granule lumen / Neutrophil degranulation / RNA binding / extracellular region / nucleus / cytosol / cytoplasm
Similarity search - Function
Signal recognition particle alu RNA binding heterodimer, srp9/1 / Signal recognition particle, SRP9 subunit / SRP9 domain / Signal recognition particle SRP9 / Signal recognition particle 9 kDa protein (SRP9) / Signal recognition particle, SRP14 subunit / Signal recognition particle, SRP9/SRP14 subunit / Signal recognition particle 14kD protein / Signal recognition particle alu RNA binding heterodimer, srp9/1 / 2-Layer Sandwich / Alpha Beta
Similarity search - Domain/homology
ACETATE ION / DI(HYDROXYETHYL)ETHER / RNA / RNA (> 10) / Signal recognition particle 14 kDa protein / Signal recognition particle 9 kDa protein
Similarity search - Component
Biological speciesHOMO SAPIENS (human)
SYNTHETIC CONSTRUCT (others)
MethodX-RAY DIFFRACTION / SYNCHROTRON / MOLECULAR REPLACEMENT / Resolution: 2.04 Å
AuthorsAhl, V. / Weichenrieder, O.
CitationJournal: Mol.Cell / Year: 2015
Title: Retrotransposition and Crystal Structure of an Alu Rnp in the Ribosome-Stalling Conformation.
Authors: Ahl, V. / Keller, H. / Schmidt, S. / Weichenrieder, O.
History
DepositionSep 12, 2015Deposition site: PDBE / Processing site: PDBE
Revision 1.0Nov 11, 2015Provider: repository / Type: Initial release
Revision 1.1Dec 2, 2015Group: Database references
Revision 1.2Dec 23, 2015Group: Database references
Revision 2.0Oct 23, 2019Group: Atomic model / Data collection ...Atomic model / Data collection / Derived calculations / Other
Category: atom_site / pdbx_database_status / struct_conn
Item: _atom_site.B_iso_or_equiv / _atom_site.Cartn_x ..._atom_site.B_iso_or_equiv / _atom_site.Cartn_x / _atom_site.Cartn_y / _atom_site.Cartn_z / _pdbx_database_status.status_code_sf / _struct_conn.pdbx_leaving_atom_flag
Revision 2.1Jan 10, 2024Group: Data collection / Database references ...Data collection / Database references / Derived calculations / Refinement description
Category: chem_comp_atom / chem_comp_bond ...chem_comp_atom / chem_comp_bond / database_2 / pdbx_initial_refinement_model / pdbx_struct_conn_angle / struct_conn / struct_site
Item: _database_2.pdbx_DOI / _database_2.pdbx_database_accession ..._database_2.pdbx_DOI / _database_2.pdbx_database_accession / _pdbx_struct_conn_angle.ptnr1_auth_comp_id / _pdbx_struct_conn_angle.ptnr1_auth_seq_id / _pdbx_struct_conn_angle.ptnr1_label_asym_id / _pdbx_struct_conn_angle.ptnr1_label_atom_id / _pdbx_struct_conn_angle.ptnr1_label_comp_id / _pdbx_struct_conn_angle.ptnr1_label_seq_id / _pdbx_struct_conn_angle.ptnr2_auth_seq_id / _pdbx_struct_conn_angle.ptnr2_label_asym_id / _pdbx_struct_conn_angle.ptnr3_auth_comp_id / _pdbx_struct_conn_angle.ptnr3_auth_seq_id / _pdbx_struct_conn_angle.ptnr3_label_asym_id / _pdbx_struct_conn_angle.ptnr3_label_atom_id / _pdbx_struct_conn_angle.ptnr3_label_comp_id / _pdbx_struct_conn_angle.ptnr3_label_seq_id / _pdbx_struct_conn_angle.value / _struct_conn.conn_type_id / _struct_conn.id / _struct_conn.pdbx_dist_value / _struct_conn.pdbx_leaving_atom_flag / _struct_conn.ptnr1_auth_asym_id / _struct_conn.ptnr1_auth_comp_id / _struct_conn.ptnr1_auth_seq_id / _struct_conn.ptnr1_label_asym_id / _struct_conn.ptnr1_label_atom_id / _struct_conn.ptnr1_label_comp_id / _struct_conn.ptnr1_label_seq_id / _struct_conn.ptnr2_auth_asym_id / _struct_conn.ptnr2_auth_comp_id / _struct_conn.ptnr2_auth_seq_id / _struct_conn.ptnr2_label_asym_id / _struct_conn.ptnr2_label_atom_id / _struct_conn.ptnr2_label_comp_id / _struct_conn.ptnr2_label_seq_id / _struct_site.pdbx_auth_asym_id / _struct_site.pdbx_auth_comp_id / _struct_site.pdbx_auth_seq_id
Remark 650 HELIX DETERMINATION METHOD: AUTHOR PROVIDED.

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Structure visualization

Structure viewerMolecule:
MolmilJmol/JSmol

Downloads & links

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Assembly

Deposited unit
A: SIGNAL RECOGNITION PARTICLE 9 KDA PROTEIN
B: SIGNAL RECOGNITION PARTICLE 14 KDA PROTEIN
C: ALU JO CONSENSUS RNA
D: SIGNAL RECOGNITION PARTICLE 9 KDA PROTEIN
E: SIGNAL RECOGNITION PARTICLE 14 KDA PROTEIN
F: ALU JO CONSENSUS RNA
hetero molecules


Theoretical massNumber of molelcules
Total (without water)98,06315
Polymers97,5496
Non-polymers5149
Water6,864381
1
A: SIGNAL RECOGNITION PARTICLE 9 KDA PROTEIN
B: SIGNAL RECOGNITION PARTICLE 14 KDA PROTEIN
C: ALU JO CONSENSUS RNA
hetero molecules


Theoretical massNumber of molelcules
Total (without water)49,1099
Polymers48,7753
Non-polymers3346
Water543
TypeNameSymmetry operationNumber
identity operation1_555x,y,z1
Buried area7470 Å2
ΔGint-84.9 kcal/mol
Surface area19570 Å2
MethodPISA
2
D: SIGNAL RECOGNITION PARTICLE 9 KDA PROTEIN
E: SIGNAL RECOGNITION PARTICLE 14 KDA PROTEIN
F: ALU JO CONSENSUS RNA
hetero molecules


Theoretical massNumber of molelcules
Total (without water)48,9546
Polymers48,7753
Non-polymers1793
Water543
TypeNameSymmetry operationNumber
identity operation1_555x,y,z1
Buried area6790 Å2
ΔGint-69.7 kcal/mol
Surface area19510 Å2
MethodPISA
Unit cell
Length a, b, c (Å)81.480, 56.730, 170.590
Angle α, β, γ (deg.)90.00, 93.69, 90.00
Int Tables number5
Space group name H-MC121

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Components

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SIGNAL RECOGNITION PARTICLE ... , 2 types, 4 molecules ADBE

#1: Protein SIGNAL RECOGNITION PARTICLE 9 KDA PROTEIN / SRP9


Mass: 9980.502 Da / Num. of mol.: 2 / Fragment: UNP RESIDUES 2-86 / Mutation: YES
Source method: isolated from a genetically manipulated source
Source: (gene. exp.) HOMO SAPIENS (human) / Plasmid: PET3A / Production host: ESCHERICHIA COLI (E. coli) / Strain (production host): BL21(DE3) / Variant (production host): ROSETTA II / References: UniProt: P49458
#2: Protein SIGNAL RECOGNITION PARTICLE 14 KDA PROTEIN / SRP14 / 18 KDA ALU RNA-BINDING PROTEIN / SRP14


Mass: 10555.262 Da / Num. of mol.: 2 / Fragment: UNP RESIDUES 2-95 / Mutation: YES
Source method: isolated from a genetically manipulated source
Source: (gene. exp.) HOMO SAPIENS (human) / Plasmid: PETM41 / Production host: ESCHERICHIA COLI (E. coli) / Strain (production host): BL21(DE3) / Variant (production host): ROSETTA II / References: UniProt: P37108

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RNA chain , 1 types, 2 molecules CF

#3: RNA chain ALU JO CONSENSUS RNA


Mass: 28238.771 Da / Num. of mol.: 2 / Fragment: ALU JO LEFT TRUNCATED MONOMER / Source method: obtained synthetically / Source: (synth.) SYNTHETIC CONSTRUCT (others)

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Non-polymers , 5 types, 390 molecules

#4: Chemical ChemComp-ACT / ACETATE ION / Acetate


Mass: 59.044 Da / Num. of mol.: 2 / Source method: obtained synthetically / Formula: C2H3O2
#5: Chemical ChemComp-SO4 / SULFATE ION / Sulfate


Mass: 96.063 Da / Num. of mol.: 2 / Source method: obtained synthetically / Formula: SO4
#6: Chemical ChemComp-PEG / DI(HYDROXYETHYL)ETHER / Diethylene glycol


Mass: 106.120 Da / Num. of mol.: 1 / Source method: obtained synthetically / Formula: C4H10O3
#7: Chemical
ChemComp-MG / MAGNESIUM ION


Mass: 24.305 Da / Num. of mol.: 4 / Source method: obtained synthetically / Formula: Mg
#8: Water ChemComp-HOH / water / Water


Mass: 18.015 Da / Num. of mol.: 381 / Source method: isolated from a natural source / Formula: H2O

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Details

Sequence detailsTRUNCATED HUMAN LEFT ALU JO MONOMER CONSENSUS SEQUENCE

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Experimental details

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Experiment

ExperimentMethod: X-RAY DIFFRACTION / Number of used crystals: 1

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Sample preparation

CrystalDensity Matthews: 2.01 Å3/Da / Density % sol: 38.84 % / Description: NONE
Crystal growpH: 6.4 / Details: 0.1M NA-MES,PH=6.4, 30%PEG400

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Data collection

DiffractionMean temperature: 100 K
Diffraction sourceSource: SYNCHROTRON / Site: SLS / Beamline: X06DA / Wavelength: 1
DetectorType: MARRESEARCH / Detector: CCD / Date: Nov 3, 2011 / Details: DYNAMICALLY BENDABLE MIRRORS
RadiationMonochromator: SI(111) / Protocol: SINGLE WAVELENGTH / Monochromatic (M) / Laue (L): M / Scattering type: x-ray
Radiation wavelengthWavelength: 1 Å / Relative weight: 1
ReflectionResolution: 2.04→56.8 Å / Num. obs: 49534 / % possible obs: 99.4 % / Observed criterion σ(I): -3 / Redundancy: 3.55 % / Biso Wilson estimate: 33.8 Å2 / Rsym value: 0.05 / Net I/σ(I): 21.45
Reflection shellResolution: 2.04→2.09 Å / Redundancy: 3.18 % / Mean I/σ(I) obs: 2.03 / Rsym value: 0.66 / % possible all: 98.1

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Processing

Software
NameVersionClassification
PHENIX(PHENIX.REFINE: 1.8.2_1309)refinement
XDSdata reduction
XSCALEdata scaling
PHASERphasing
RefinementMethod to determine structure: MOLECULAR REPLACEMENT
Starting model: PDB ENTRY 1E8O CHAINS A AND B

1e8o


Resolution: 2.04→56.746 Å / SU ML: 0.26 / σ(F): 2 / Phase error: 23.13 / Stereochemistry target values: ML
Details: THE FOLLOWING RESIDUES ARE DISORDERED. CHAIN A, RESIDUES 78 TO 86. CHAIN B, RESIDUES 41 TO 48. CHAIN C, RESIDUES 68, 69. CHAIN D, RESIDUES 75 TO 86. CHAIN E, RESIDUES 41 TO 50. CHAIN F, ...Details: THE FOLLOWING RESIDUES ARE DISORDERED. CHAIN A, RESIDUES 78 TO 86. CHAIN B, RESIDUES 41 TO 48. CHAIN C, RESIDUES 68, 69. CHAIN D, RESIDUES 75 TO 86. CHAIN E, RESIDUES 41 TO 50. CHAIN F, RESIDUES 57, 69. THE FOLLOWING RESIDUES WERE TRUNCATED AT THE CB POSITIONS. CHAIN A, RESIDUES 76, 77. CHAIN B, RESIDUE 50. MG ION COORDINATION WAS RESTRAINED. HYDROGENS WERE ADDED IN THE RIDING POSITIONS.
RfactorNum. reflection% reflection
Rfree0.222 1539 3.1 %
Rwork0.1855 --
obs0.1866 49531 99.35 %
Solvent computationShrinkage radii: 0.9 Å / VDW probe radii: 1.11 Å / Solvent model: FLAT BULK SOLVENT MODEL
Displacement parametersBiso mean: 35.9 Å2
Refinement stepCycle: LAST / Resolution: 2.04→56.746 Å
ProteinNucleic acidLigandSolventTotal
Num. atoms2557 3671 29 381 6638
Refine LS restraints
Refine-IDTypeDev idealNumber
X-RAY DIFFRACTIONf_bond_d0.0036732
X-RAY DIFFRACTIONf_angle_d0.7239938
X-RAY DIFFRACTIONf_dihedral_angle_d13.4723039
X-RAY DIFFRACTIONf_chiral_restr0.0341244
X-RAY DIFFRACTIONf_plane_restr0.003601
LS refinement shell
Resolution (Å)Rfactor RfreeNum. reflection RfreeRfactor RworkNum. reflection RworkRefine-ID% reflection obs (%)
2.0401-2.10590.33051280.28234272X-RAY DIFFRACTION98
2.1059-2.18120.30931320.26344348X-RAY DIFFRACTION99
2.1812-2.26850.26421410.25264375X-RAY DIFFRACTION99
2.2685-2.37180.29021580.24474322X-RAY DIFFRACTION100
2.3718-2.49680.28721480.234336X-RAY DIFFRACTION100
2.4968-2.65320.24381270.22544331X-RAY DIFFRACTION100
2.6532-2.85810.25371370.21234380X-RAY DIFFRACTION100
2.8581-3.14570.22741510.18984390X-RAY DIFFRACTION100
3.1457-3.60080.19021480.15664351X-RAY DIFFRACTION99
3.6008-4.53630.18721380.14314404X-RAY DIFFRACTION99
4.5363-56.76760.18181310.16044483X-RAY DIFFRACTION99
Refinement TLS params.

Method: refined / Refine-ID: X-RAY DIFFRACTION

IDL11 (°2)L12 (°2)L13 (°2)L22 (°2)L23 (°2)L33 (°2)S11 (Å °)S12 (Å °)S13 (Å °)S21 (Å °)S22 (Å °)S23 (Å °)S31 (Å °)S32 (Å °)S33 (Å °)T11 (Å2)T12 (Å2)T13 (Å2)T22 (Å2)T23 (Å2)T33 (Å2)Origin x (Å)Origin y (Å)Origin z (Å)
11.05810.18960.74121.06780.45430.79450.2243-0.1949-0.10540.24990.0798-0.25640.3314-0.09430.6590.258-0.0991-0.0780.4413-0.12350.306128.0401-1.012234.7206
23.2189-0.3420.97440.9409-1.98444.2027-0.0874-0.25890.60180.247-0.14330.056-1.0030.2063-0.61020.313-0.05360.09010.1498-0.01430.353320.4825.465529.2256
30.02850.00310.01830.04460.03430.0354-0.03730.6874-0.2061-0.2994-0.2856-0.1235-0.29010.12060.00040.33260.04360.03890.2730.04610.407716.42540.615616.6565
41.00330.1993-0.3120.86760.36580.43650.1626-0.21730.02350.1752-0.057-0.1178-0.0950.3212-0.00530.16650.0046-0.01930.1815-0.02210.197619.9001-7.716633.0103
50.34650.4645-0.44180.6706-1.01484.42260.0228-0.4190.37310.3558-0.03590.297-0.5528-0.92891.17090.2669-0.02470.04590.3261-0.11980.267811.00821.342633.4688
63.72964.8902-6.51147.0152-9.689120.06880.17330.1817-0.3041-0.0048-0.1284-0.4191-0.05370.1330.57920.0511-0.09960.22790.09090.38455.59878.901917.3842
70.0049-0.0341-0.06731.1315-0.19832.55280.0256-0.2398-0.3787-0.01640.04620.41490.2787-0.8639-0.16190.1909-0.01610.02490.26170.02240.27272.3443-15.351632.3417
80.78230.1602-0.17140.3940.42630.8925-0.02430.1074-0.05980.08060.01750.2098-0.004-0.1339-0.09760.13170.0476-0.00370.1409-0.00440.1977.4367-10.344125.331
90.66010.75780.18933.3077-0.64630.36040.35790.36570.0132-1.1337-0.4926-0.59890.1621-0.16550.02780.35260.08770.01370.30270.04670.37067.9122-7.01169.52
100.0243-0.0311-0.06510.06770.05070.20160.04940.1460.0618-0.1457-0.0912-0.0559-0.03480.02070.30660.4760.2443-0.01160.43370.13940.33654.220.991510.8241
111.7620.0109-0.96211.955-0.32750.5801-0.2077-0.40710.18130.5279-0.1231-0.0487-0.3106-0.2228-0.72060.22380.0470.01150.1687-0.00910.24084.7937-4.773737.4599
120.79670.18050.11661.30490.06330.963-0.03050.1994-0.2096-0.0583-0.02750.01670.2573-0.13820.01770.160.00820.02210.1731-0.04510.243715.5731-26.890217.4699
130.4312-0.35690.19120.3496-0.03820.33120.17150.27320.7393-0.1917-0.03640.3845-0.22360.08630.00090.3744-0.06720.10190.3052-0.04870.452521.4129-37.01049.0565
141.55330.1562-0.66741.76080.23211.4239-0.12940.242-0.1343-0.11770.0128-0.211-0.07910.3081-0.11310.16480.02550.01670.2395-0.00080.213330.3371-15.694313.5994
154.2256-1.454-1.98421.2736-0.04983.37460.0705-0.44950.7098-0.3934-0.3143-0.1942-1.31470.67140.67150.8172-0.0121-0.24660.67710.01850.475345.6326-17.723338.8026
160.3881-0.0824-0.30090.59820.16690.2515-0.45930.247-0.0928-0.2112-0.24970.1193-0.5182-0.4634-0.10260.7570.0307-0.18260.32760.04080.624851.7656-3.478546.7611
170.29150.3471-0.2553.09920.86791.1515-0.0146-0.01540.3304-0.89740.25530.0143-0.27160.03930.22920.5026-0.0283-0.04080.2889-0.0911.147758.82123.068852.6291
180.0116-0.02410.02750.0497-0.05670.06450.0445-0.21040.29850.0440.00340.3562-0.0342-0.14040.14130.274-0.09170.03660.6379-0.29770.739661.2769-0.469966.584
191.0425-0.86910.3371.18120.10830.4471-0.17730.1920.4739-0.5069-0.07820.3491-0.1268-0.1198-0.24680.4454-0.1126-0.05290.2472-0.06670.398159.5913-10.304550.5591
200.2453-0.07740.30940.3391-0.04620.3976-0.21860.3050.6012-0.434-0.120.4164-0.04540.4076-0.02020.5328-0.13050.01420.32320.08780.612468.7097-1.287750.1394
211.12850.1931.64731.4615-0.60252.9558-0.2327-0.2573-0.4003-0.15790.2014-0.76490.10740.808-0.43270.2621-0.05550.0560.5768-0.14490.387676.7872-17.83453.9426
220.2255-0.25370.4250.2912-0.48420.7994-0.066-0.29910.2082-0.00320.1612-0.3332-0.15650.39590.06060.3315-0.10980.03910.3757-0.15250.310471.1004-12.108659.8973
230.1450.0449-0.08240.0135-0.02530.04650.1327-0.0708-0.0546-0.02480.0649-0.07030.16620.11981.22830.398-0.1356-0.05850.6639-0.27160.444668.7257-7.457575.1557
240.0397-0.02510.01760.016-0.01110.00780.01740.00270.0251-0.01770.00770.06910.0408-0.01030.79061.0259-0.3494-0.2430.7915-0.34931.038372.74071.809770.9437
253.9328-0.30662.75660.16480.00872.2866-0.63690.43330.8442-0.58260.0862-0.188-0.2170.0517-0.41580.6141-0.25260.05440.4572-0.00320.470574.9034-7.654747.7324
260.8305-0.833-0.25121.0227-0.29311.9369-0.155-0.6127-0.0530.08980.1201-0.07560.2575-0.0413-0.06710.2942-0.05660.00840.4523-0.03460.152462.325-28.285168.4132
271.6633-1.4503-0.86322.27180.51670.50370.1634-0.33010.20350.0796-0.08-0.2379-0.0527-0.5810.07670.4697-0.02560.10610.82340.14770.349455.7083-37.802576.7989
280.19520.5547-0.12571.97210.14061.98-0.1491-0.51630.301-0.03870.01740.359-0.1379-0.3770.16550.32270.0425-0.03880.7188-0.19420.323347.3542-16.561469.3623
297.82071.1996-6.08372.36840.58177.93780.2243-0.00131.6329-0.1747-0.1590.5311-1.9172-0.65132.44730.93370.1342-0.34070.6869-0.0160.652636.0876-15.645742.1433
Refinement TLS group
IDRefine-IDRefine TLS-IDSelection details
1X-RAY DIFFRACTION1CHAIN 'A' AND (RESSEQ 2:6)
2X-RAY DIFFRACTION2CHAIN 'A' AND (RESSEQ 7:20)
3X-RAY DIFFRACTION3CHAIN 'A' AND (RESSEQ 21:25)
4X-RAY DIFFRACTION4CHAIN 'A' AND (RESSEQ 26:55)
5X-RAY DIFFRACTION5CHAIN 'A' AND (RESSEQ 56:74)
6X-RAY DIFFRACTION6CHAIN 'A' AND (RESSEQ 75:77)
7X-RAY DIFFRACTION7CHAIN 'B' AND (RESSEQ 2:22)
8X-RAY DIFFRACTION8CHAIN 'B' AND ((RESSEQ 23:32) OR (RESSEQ 55:75))
9X-RAY DIFFRACTION9CHAIN 'B' AND (RESSEQ 33:40)
10X-RAY DIFFRACTION10CHAIN 'B' AND (RESSEQ 49:54)
11X-RAY DIFFRACTION11CHAIN 'B' AND (RESSEQ 76:95)
12X-RAY DIFFRACTION12CHAIN 'C' AND ((RESSEQ 1:33) OR (RESSEQ 41:48))
13X-RAY DIFFRACTION13CHAIN 'C' AND (RESSEQ 34:40)
14X-RAY DIFFRACTION14CHAIN 'C' AND ((RESSEQ 49:64) OR (RESSEQ 102:118))
15X-RAY DIFFRACTION15CHAIN 'C' AND ((RESSEQ 65:69) OR (RESSEQ 99:101))
16X-RAY DIFFRACTION16CHAIN 'D' AND (RESSEQ 2:6)
17X-RAY DIFFRACTION17CHAIN 'D' AND (RESSEQ 7:20)
18X-RAY DIFFRACTION18CHAIN 'D' AND (RESSEQ 21:25)
19X-RAY DIFFRACTION19CHAIN 'D' AND (RESSEQ 26:55)
20X-RAY DIFFRACTION20CHAIN 'D' AND (RESSEQ 56:74)
21X-RAY DIFFRACTION21CHAIN 'E' AND (RESSEQ 2:22)
22X-RAY DIFFRACTION22CHAIN 'E' AND ((RESSEQ 23:32) OR (RESSEQ 55:75))
23X-RAY DIFFRACTION23CHAIN 'E' AND (RESSEQ 33:40)
24X-RAY DIFFRACTION24CHAIN 'E' AND (RESSEQ 51:54)
25X-RAY DIFFRACTION25CHAIN 'E' AND (RESSEQ 76:95)
26X-RAY DIFFRACTION26CHAIN 'F' AND ((RESSEQ 1:33) OR (RESSEQ 41:48))
27X-RAY DIFFRACTION27CHAIN 'F' AND (RESSEQ 34:40)
28X-RAY DIFFRACTION28CHAIN 'F' AND ((RESSEQ 49:64) OR (RESSEQ 102:118))
29X-RAY DIFFRACTION29CHAIN 'F' AND ((RESSEQ 65:69) OR (RESSEQ 99:101))

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