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Yorodumi- PDB-5al7: N-terminal fragment of Drosophila melanogaster Sas-6 (F143D), dim... -
+Open data
-Basic information
Entry | Database: PDB / ID: 5al7 | ||||||
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Title | N-terminal fragment of Drosophila melanogaster Sas-6 (F143D), dimerised via the coiled-coil domain. | ||||||
Components | SPINDLE ASSEMBLY ABNORMAL PROTEIN 6 HOMOLOG | ||||||
Keywords | STRUCTURAL PROTEIN / CENTRIOLE / CARTWHEEL | ||||||
Function / homology | Function and homology information ciliary basal body organization / centriole assembly / centriole-centriole cohesion / centrosome cycle / centriole replication / centrosome duplication / centriole / ciliary basal body / centrosome / cytoplasm Similarity search - Function | ||||||
Biological species | DROSOPHILA MELANOGASTER (fruit fly) | ||||||
Method | X-RAY DIFFRACTION / SYNCHROTRON / MOLECULAR REPLACEMENT / Resolution: 2.92 Å | ||||||
Authors | Cottee, M.A. / Johnson, S. / Lea, S.M. | ||||||
Citation | Journal: Elife / Year: 2015 Title: The homo-oligomerisation of both Sas-6 and Ana2 is required for efficient centriole assembly in flies. Authors: Cottee, M.A. / Muschalik, N. / Johnson, S. / Leveson, J. / Raff, J.W. / Lea, S.M. | ||||||
History |
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Remark 700 | SHEET DETERMINATION METHOD: DSSP THE SHEETS PRESENTED AS "AA" IN EACH CHAIN ON SHEET RECORDS BELOW ... SHEET DETERMINATION METHOD: DSSP THE SHEETS PRESENTED AS "AA" IN EACH CHAIN ON SHEET RECORDS BELOW IS ACTUALLY AN 7-STRANDED BARREL THIS IS REPRESENTED BY A 8-STRANDED SHEET IN WHICH THE FIRST AND LAST STRANDS ARE IDENTICAL. THE SHEETS PRESENTED AS "BA" IN EACH CHAIN ON SHEET RECORDS BELOW IS ACTUALLY AN 7-STRANDED BARREL THIS IS REPRESENTED BY A 8-STRANDED SHEET IN WHICH THE FIRST AND LAST STRANDS ARE IDENTICAL. |
-Structure visualization
Structure viewer | Molecule: MolmilJmol/JSmol |
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-Downloads & links
-Download
PDBx/mmCIF format | 5al7.cif.gz | 182.2 KB | Display | PDBx/mmCIF format |
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PDB format | pdb5al7.ent.gz | 147.5 KB | Display | PDB format |
PDBx/mmJSON format | 5al7.json.gz | Tree view | PDBx/mmJSON format | |
Others | Other downloads |
-Validation report
Arichive directory | https://data.pdbj.org/pub/pdb/validation_reports/al/5al7 ftp://data.pdbj.org/pub/pdb/validation_reports/al/5al7 | HTTPS FTP |
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-Related structure data
Related structure data | 5al6C 2y3vS 2y3wS 3q0xS C: citing same article (ref.) S: Starting model for refinement |
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Similar structure data |
-Links
-Assembly
Deposited unit |
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1 |
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Unit cell |
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Noncrystallographic symmetry (NCS) | NCS oper:
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-Components
#1: Protein | Mass: 25168.453 Da / Num. of mol.: 2 Fragment: N-TERMINAL HEAD GROUP PLUS COILED-COIL, UNP RESIDUES 2-216 Mutation: YES Source method: isolated from a genetically manipulated source Source: (gene. exp.) DROSOPHILA MELANOGASTER (fruit fly) / Plasmid: PETM-14 / Production host: ESCHERICHIA COLI B (bacteria) / Variant (production host): B834 (DE3) / References: UniProt: Q9VAC8 #2: Chemical | #3: Water | ChemComp-HOH / | Sequence details | AAL68137 VARIANT, E36D, I207L | |
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-Experimental details
-Experiment
Experiment | Method: X-RAY DIFFRACTION / Number of used crystals: 1 |
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-Sample preparation
Crystal | Density Matthews: 3.74 Å3/Da / Density % sol: 67.09 % / Description: NONE |
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Crystal grow | pH: 7.5 Details: 0.1M BICINE/TRIZMA MIX (PH 8.5), 20% W/V PEG 550MME, 10% W/V PEG 20K, 30 MM NANO3, 30 MM NA2HPO4, 30 MM (NH4)2SO4 |
-Data collection
Diffraction | Mean temperature: 100 K |
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Diffraction source | Source: SYNCHROTRON / Site: ESRF / Beamline: ID23-2 / Wavelength: 0.8726 |
Detector | Date: Sep 10, 2011 |
Radiation | Protocol: SINGLE WAVELENGTH / Monochromatic (M) / Laue (L): M / Scattering type: x-ray |
Radiation wavelength | Wavelength: 0.8726 Å / Relative weight: 1 |
Reflection | Resolution: 2.92→41.43 Å / Num. obs: 15767 / % possible obs: 97.4 % / Observed criterion σ(I): 2 / Redundancy: 5.2 % / Biso Wilson estimate: 43.17 Å2 / Rmerge(I) obs: 0.13 / Net I/σ(I): 12.1 |
Reflection shell | Resolution: 2.92→3 Å / Redundancy: 5.1 % / Rmerge(I) obs: 0.67 / Mean I/σ(I) obs: 2.6 / % possible all: 96.4 |
-Processing
Software |
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Refinement | Method to determine structure: MOLECULAR REPLACEMENT Starting model: ENSEMBLE OF PDB ENTRIES 2Y3V A,B,D, 2Y3W A, B 3Q0X A,B Resolution: 2.92→41.429 Å / SU ML: 0.31 / σ(F): 1.34 / Phase error: 24.84 / Stereochemistry target values: ML
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Solvent computation | Shrinkage radii: 0.9 Å / VDW probe radii: 1.11 Å / Solvent model: FLAT BULK SOLVENT MODEL | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Refinement step | Cycle: LAST / Resolution: 2.92→41.429 Å
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Refine LS restraints |
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LS refinement shell |
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Refinement TLS params. | Method: refined / Refine-ID: X-RAY DIFFRACTION
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Refinement TLS group |
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