+Open data
-Basic information
Entry | Database: PDB / ID: 4zxg | ||||||
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Title | Ligandin binding site of PfGST | ||||||
Components | Glutathione S-transferase | ||||||
Keywords | TRANSFERASE / GST / Plasmodium falciparum / Hemin | ||||||
Function / homology | Function and homology information Glutathione conjugation / Synthesis of Prostaglandins (PG) and Thromboxanes (TX) / glutathione transferase / glutathione transferase activity Similarity search - Function | ||||||
Biological species | Plasmodium falciparum (malaria parasite P. falciparum) | ||||||
Method | X-RAY DIFFRACTION / SYNCHROTRON / MOLECULAR REPLACEMENT / Resolution: 1.7 Å | ||||||
Authors | Perbandt, M. / Eberle, R. / Betzel, C. | ||||||
Citation | Journal: J.Struct.Biol. / Year: 2015 Title: High resolution structures of Plasmodium falciparum GST complexes provide novel insights into the dimer-tetramer transition and a novel ligand-binding site. Authors: Perbandt, M. / Eberle, R. / Fischer-Riepe, L. / Cang, H. / Liebau, E. / Betzel, C. | ||||||
History |
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-Structure visualization
Structure viewer | Molecule: MolmilJmol/JSmol |
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-Downloads & links
-Download
PDBx/mmCIF format | 4zxg.cif.gz | 100.4 KB | Display | PDBx/mmCIF format |
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PDB format | pdb4zxg.ent.gz | 76.1 KB | Display | PDB format |
PDBx/mmJSON format | 4zxg.json.gz | Tree view | PDBx/mmJSON format | |
Others | Other downloads |
-Validation report
Arichive directory | https://data.pdbj.org/pub/pdb/validation_reports/zx/4zxg ftp://data.pdbj.org/pub/pdb/validation_reports/zx/4zxg | HTTPS FTP |
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-Related structure data
Related structure data | 1pa3S S: Starting model for refinement |
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Similar structure data |
-Links
-Assembly
Deposited unit |
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Unit cell |
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Components on special symmetry positions |
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-Components
#1: Protein | Mass: 24150.293 Da / Num. of mol.: 2 / Fragment: UNP residues 3-207 Source method: isolated from a genetically manipulated source Source: (gene. exp.) Plasmodium falciparum (malaria parasite P. falciparum) Gene: GST / Production host: Escherichia coli (E. coli) / References: UniProt: Q8MU52, glutathione transferase #2: Chemical | ChemComp-SO4 / #3: Chemical | #4: Chemical | #5: Water | ChemComp-HOH / | |
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-Experimental details
-Experiment
Experiment | Method: X-RAY DIFFRACTION / Number of used crystals: 1 |
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-Sample preparation
Crystal | Density Matthews: 2.04 Å3/Da / Density % sol: 39.62 % |
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Crystal grow | Temperature: 293 K / Method: vapor diffusion, hanging drop / Details: ammonium sulfate, MES |
-Data collection
Diffraction | Mean temperature: 100 K |
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Diffraction source | Source: SYNCHROTRON / Site: PETRA III, EMBL c/o DESY / Beamline: P14 (MX2) / Wavelength: 1.24 Å |
Detector | Type: PSI PILATUS 6M / Detector: PIXEL / Date: Oct 27, 2012 |
Radiation | Protocol: SINGLE WAVELENGTH / Monochromatic (M) / Laue (L): M / Scattering type: x-ray |
Radiation wavelength | Wavelength: 1.24 Å / Relative weight: 1 |
Reflection | Resolution: 1.7→30 Å / Num. obs: 273546 / % possible obs: 98.3 % / Redundancy: 6.2 % / Rmerge(I) obs: 0.026 / Net I/σ(I): 30.5 |
-Processing
Software |
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Refinement | Method to determine structure: MOLECULAR REPLACEMENT Starting model: 1PA3 Resolution: 1.7→30 Å / Cross valid method: FREE R-VALUE
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Displacement parameters | Biso max: 98.51 Å2 / Biso mean: 34.456 Å2 / Biso min: 18.11 Å2 | ||||||||||||||||||
Refinement step | Cycle: LAST / Resolution: 1.7→30 Å
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