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Yorodumi- PDB-4l65: Crystal structure of the Candida albicans Methionine Synthase in ... -
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-Basic information
Entry | Database: PDB / ID: 4l65 | ||||||
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Title | Crystal structure of the Candida albicans Methionine Synthase in complex with 5-Methyl-Tetrahydrofolate and Methionine | ||||||
Components | 5-methyltetrahydropteroyltriglutamate--homocysteine methyltransferase | ||||||
Keywords | TRANSFERASE / cobalamin-independent / surface entropy reduction / fungal / Dual TIM Barrels / Methionine synthase | ||||||
Function / homology | Function and homology information L-methionine biosynthetic process from homoserine via O-acetyl-L-homoserine and cystathionine / : / 5-methyltetrahydropteroyltriglutamate-homocysteine S-methyltransferase / 5-methyltetrahydropteroyltriglutamate-homocysteine S-methyltransferase activity / fungal biofilm matrix / hyphal cell wall / adenine biosynthetic process / methionine metabolic process / 'de novo' L-methionine biosynthetic process / fungal-type cell wall ...L-methionine biosynthetic process from homoserine via O-acetyl-L-homoserine and cystathionine / : / 5-methyltetrahydropteroyltriglutamate-homocysteine S-methyltransferase / 5-methyltetrahydropteroyltriglutamate-homocysteine S-methyltransferase activity / fungal biofilm matrix / hyphal cell wall / adenine biosynthetic process / methionine metabolic process / 'de novo' L-methionine biosynthetic process / fungal-type cell wall / methionine biosynthetic process / cellular response to heat / methylation / cell surface / zinc ion binding / nucleus Similarity search - Function | ||||||
Biological species | Candida albicans SC5314 (yeast) | ||||||
Method | X-RAY DIFFRACTION / SYNCHROTRON / MOLECULAR REPLACEMENT / Resolution: 2.31 Å | ||||||
Authors | Ubhi, D. / Robertus, J.D. | ||||||
Citation | Journal: J.Mol.Biol. / Year: 2014 Title: Structural analysis of a fungal methionine synthase with substrates and inhibitors. Authors: Ubhi, D. / Kago, G. / Monzingo, A.F. / Robertus, J.D. | ||||||
History |
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-Structure visualization
Structure viewer | Molecule: MolmilJmol/JSmol |
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-Downloads & links
-Download
PDBx/mmCIF format | 4l65.cif.gz | 301.2 KB | Display | PDBx/mmCIF format |
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PDB format | pdb4l65.ent.gz | 241.5 KB | Display | PDB format |
PDBx/mmJSON format | 4l65.json.gz | Tree view | PDBx/mmJSON format | |
Others | Other downloads |
-Validation report
Arichive directory | https://data.pdbj.org/pub/pdb/validation_reports/l6/4l65 ftp://data.pdbj.org/pub/pdb/validation_reports/l6/4l65 | HTTPS FTP |
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-Related structure data
-Links
-Assembly
Deposited unit |
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1 |
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Unit cell |
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-Components
#1: Protein | Mass: 88188.961 Da / Num. of mol.: 1 / Mutation: K103A, K104A, E107A Source method: isolated from a genetically manipulated source Source: (gene. exp.) Candida albicans SC5314 (yeast) / Strain: BWP17 / Gene: CaO19.10083, CaO19.2551, MET6 / Plasmid: pNIC28-Bsa4 / Production host: Escherichia coli (E. coli) / Strain (production host): BL21(DE3) References: UniProt: P82610, 5-methyltetrahydropteroyltriglutamate-homocysteine S-methyltransferase |
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#2: Chemical | ChemComp-ZN / |
#3: Chemical | ChemComp-C2F / |
#4: Chemical | ChemComp-MET / |
#5: Water | ChemComp-HOH / |
-Experimental details
-Experiment
Experiment | Method: X-RAY DIFFRACTION / Number of used crystals: 1 |
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-Sample preparation
Crystal | Density Matthews: 2.18 Å3/Da / Density % sol: 43.49 % |
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Crystal grow | Temperature: 293 K / Method: vapor diffusion, hanging drop / pH: 7.4 Details: 50 mM NaI, 27% (w/v) PEG 3350, 0.25 mM DTT, 0.15 mM ZnSO4, 5 mM 5-Methyl-Tetrahydrofolate-Glu3, 10 mM Methionine, 20 mM Tris-Cl pH 7.4 , VAPOR DIFFUSION, HANGING DROP, temperature 293K |
-Data collection
Diffraction | Mean temperature: 100 K |
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Diffraction source | Source: SYNCHROTRON / Site: ALS / Beamline: 5.0.3 / Wavelength: 0.97648 Å |
Detector | Type: ADSC QUANTUM 315r / Detector: CCD / Date: May 13, 2012 / Details: Asymmetric cut single crystal Si (220) |
Radiation | Monochromator: Asymmetric cut single crystal Si (220) / Protocol: SINGLE WAVELENGTH / Monochromatic (M) / Laue (L): M / Scattering type: x-ray |
Radiation wavelength | Wavelength: 0.97648 Å / Relative weight: 1 |
Reflection | Resolution: 2.3→49.52 Å / Num. all: 34148 / Num. obs: 34134 / % possible obs: 100 % / Observed criterion σ(I): 0 / Redundancy: 7.2 % / Rmerge(I) obs: 0.066 / Net I/σ(I): 11 |
Reflection shell | Resolution: 2.33→2.37 Å / Rmerge(I) obs: 0.549 / % possible all: 99.8 |
-Processing
Software |
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Refinement | Method to determine structure: MOLECULAR REPLACEMENT / Resolution: 2.31→49.52 Å / Cor.coef. Fo:Fc: 0.949 / Cor.coef. Fo:Fc free: 0.903 / SU B: 16.051 / SU ML: 0.197 / Cross valid method: THROUGHOUT / ESU R: 0.381 / ESU R Free: 0.274 / Stereochemistry target values: MAXIMUM LIKELIHOOD / Details: HYDROGENS HAVE BEEN ADDED IN THE RIDING POSITIONS
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Solvent computation | Ion probe radii: 0.8 Å / Shrinkage radii: 0.8 Å / VDW probe radii: 1.2 Å / Solvent model: MASK | |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Displacement parameters | Biso mean: 56.832 Å2
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Refinement step | Cycle: LAST / Resolution: 2.31→49.52 Å
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Refine LS restraints |
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LS refinement shell | Resolution: 2.314→2.374 Å / Total num. of bins used: 20
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Refinement TLS params. | Method: refined / Refine-ID: X-RAY DIFFRACTION
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Refinement TLS group |
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