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Yorodumi- PDB-4zbh: THE CRYSTAL STRUCTURE OF THE SOLUBLE DOMAIN OF SULFOLOBUS ACIDOCA... -
+Open data
-Basic information
Entry | Database: PDB / ID: 4zbh | ||||||
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Title | THE CRYSTAL STRUCTURE OF THE SOLUBLE DOMAIN OF SULFOLOBUS ACIDOCALDARIUS FLAF | ||||||
Components | Conserved flagellar protein F | ||||||
Keywords | IMMUNE SYSTEM / IMMUNOGLOBULIN-LIKE BETA-SANDWICH / stator protein | ||||||
Function / homology | membrane / identical protein binding / Conserved flagellar protein F Function and homology information | ||||||
Biological species | Sulfolobus acidocaldarius (acidophilic) | ||||||
Method | X-RAY DIFFRACTION / SYNCHROTRON / SIRAS / Resolution: 1.5 Å | ||||||
Authors | Tsai, C.-L. / Arvai, A.S. / Ishida, J.P. / Tainer, J.A. | ||||||
Citation | Journal: Structure / Year: 2015 Title: FlaF Is a beta-Sandwich Protein that Anchors the Archaellum in the Archaeal Cell Envelope by Binding the S-Layer Protein. Authors: Banerjee, A. / Tsai, C.L. / Chaudhury, P. / Tripp, P. / Arvai, A.S. / Ishida, J.P. / Tainer, J.A. / Albers, S.V. | ||||||
History |
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-Structure visualization
Structure viewer | Molecule: MolmilJmol/JSmol |
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-Downloads & links
-Download
PDBx/mmCIF format | 4zbh.cif.gz | 69.1 KB | Display | PDBx/mmCIF format |
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PDB format | pdb4zbh.ent.gz | 50.2 KB | Display | PDB format |
PDBx/mmJSON format | 4zbh.json.gz | Tree view | PDBx/mmJSON format | |
Others | Other downloads |
-Validation report
Arichive directory | https://data.pdbj.org/pub/pdb/validation_reports/zb/4zbh ftp://data.pdbj.org/pub/pdb/validation_reports/zb/4zbh | HTTPS FTP |
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-Related structure data
-Links
-Assembly
Deposited unit |
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1 |
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Unit cell |
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Details | Dimer according to SAXS and gel filtration |
-Components
#1: Protein | Mass: 16248.860 Da / Num. of mol.: 1 / Fragment: RESIDUES 35-164 Source method: isolated from a genetically manipulated source Source: (gene. exp.) Sulfolobus acidocaldarius (strain ATCC 33909 / DSM 639 / JCM 8929 / NBRC 15157 / NCIMB 11770) (acidophilic) Strain: ATCC 33909 / DSM 639 / JCM 8929 / NBRC 15157 / NCIMB 11770 Gene: Saci_1175 / Plasmid: PET / Production host: Escherichia coli (E. coli) / References: UniProt: Q4J9K8 |
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#2: Water | ChemComp-HOH / |
-Experimental details
-Experiment
Experiment | Method: X-RAY DIFFRACTION / Number of used crystals: 2 |
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-Sample preparation
Crystal | Density Matthews: 2.12 Å3/Da / Density % sol: 41.92 % |
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Crystal grow | Temperature: 288 K / Method: vapor diffusion, hanging drop / pH: 7 Details: 3 M POTASSIUM CHLORIDE AND 50 mM HEPES, PH7.0, VAPOR DIFFUSION, HANGING DROP, TEMPERATURE 298K PH range: 7 |
-Data collection
Diffraction | Mean temperature: 100 K |
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Diffraction source | Source: SYNCHROTRON / Site: ALS / Beamline: 12.3.1 / Wavelength: 0.9677 Å |
Detector | Type: ADSC QUANTUM 315r / Detector: CCD / Date: Aug 8, 2012 |
Radiation | Protocol: SINGLE WAVELENGTH / Monochromatic (M) / Laue (L): M / Scattering type: x-ray |
Radiation wavelength | Wavelength: 0.9677 Å / Relative weight: 1 |
Reflection | Resolution: 1.5→50 Å / Num. obs: 22800 / % possible obs: 98.4 % / Redundancy: 5.6 % / Rsym value: 0.051 / Net I/σ(I): 62.6 |
Reflection shell | Resolution: 1.5→1.55 Å / Mean I/σ(I) obs: 3.7 / % possible all: 97.6 |
-Processing
Software |
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Refinement | Method to determine structure: SIRAS / Resolution: 1.5→46.44 Å / SU ML: 0.12 / Cross valid method: FREE R-VALUE / σ(F): 0 / Phase error: 20.52 / Stereochemistry target values: ML
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Solvent computation | Shrinkage radii: 0.9 Å / VDW probe radii: 1.11 Å / Solvent model: FLAT BULK SOLVENT MODEL | |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Refinement step | Cycle: LAST / Resolution: 1.5→46.44 Å
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Refine LS restraints |
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LS refinement shell |
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