+Open data
-Basic information
Entry | Database: PDB / ID: 4xzw | ||||||
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Title | Endo-glucanase chimera C10 | ||||||
Components | endo-glucanase chimera C10 | ||||||
Keywords | HYDROLASE / endo-glucanase / crown ether | ||||||
Function / homology | Function and homology information cellulose binding / cellulase / cellulase activity / cellulose catabolic process / membrane / metal ion binding Similarity search - Function | ||||||
Biological species | uncultured bacterium (environmental samples) Geobacillus sp. 70PC53 (bacteria) | ||||||
Method | X-RAY DIFFRACTION / SYNCHROTRON / MOLECULAR REPLACEMENT / Resolution: 1.5 Å | ||||||
Authors | Lee, C.C. / Chang, C.J. / Ho, T.H.D. / Chao, Y.C. / Wang, A.H.J. | ||||||
Citation | Journal: To Be Published Title: Endo-glucanase chimera C10 Authors: Wang, A.H.J. / Lee, C.C. / Chang, C.J. / Ho, T.H.D. / Chao, Y.C. | ||||||
History |
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-Structure visualization
Structure viewer | Molecule: MolmilJmol/JSmol |
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-Downloads & links
-Download
PDBx/mmCIF format | 4xzw.cif.gz | 88.9 KB | Display | PDBx/mmCIF format |
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PDB format | pdb4xzw.ent.gz | 63.7 KB | Display | PDB format |
PDBx/mmJSON format | 4xzw.json.gz | Tree view | PDBx/mmJSON format | |
Others | Other downloads |
-Validation report
Arichive directory | https://data.pdbj.org/pub/pdb/validation_reports/xz/4xzw ftp://data.pdbj.org/pub/pdb/validation_reports/xz/4xzw | HTTPS FTP |
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-Related structure data
Related structure data | 4xzbS S: Starting model for refinement |
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Similar structure data |
-Links
-Assembly
Deposited unit |
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1 |
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Unit cell |
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-Components
#1: Protein | Mass: 35448.570 Da / Num. of mol.: 1 Source method: isolated from a genetically manipulated source Details: This chimera protein combines two UniProt entries M4I2R5 and C5H6X3 Source: (gene. exp.) uncultured bacterium (environmental samples), (gene. exp.) Geobacillus sp. 70PC53 (bacteria) Plasmid: pET21b / Gene: celA / Production host: Escherichia coli BL21(DE3) (bacteria) / Strain (production host): BL21(DE3) / References: UniProt: M4I2R5, UniProt: C5H6X3, cellulase | ||||||
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#2: Chemical | #3: Chemical | ChemComp-CA / | #4: Chemical | ChemComp-EPE / | #5: Water | ChemComp-HOH / | |
-Experimental details
-Experiment
Experiment | Method: X-RAY DIFFRACTION / Number of used crystals: 1 |
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-Sample preparation
Crystal | Density Matthews: 2.26 Å3/Da / Density % sol: 45.52 % |
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Crystal grow | Temperature: 291 K / Method: vapor diffusion / pH: 7.4 Details: 25 mM 18-crown-6, 30% (w/v) PEG 4000, 0.1 M HEPES sodium salt, pH 7.4, 0.2 M calcium chloride. |
-Data collection
Diffraction | Mean temperature: 100 K |
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Diffraction source | Source: SYNCHROTRON / Site: SPring-8 / Beamline: BL12B2 / Wavelength: 1 Å |
Detector | Type: ADSC QUANTUM 210 / Detector: CCD / Date: Dec 12, 2014 |
Radiation | Protocol: SINGLE WAVELENGTH / Monochromatic (M) / Laue (L): M / Scattering type: x-ray |
Radiation wavelength | Wavelength: 1 Å / Relative weight: 1 |
Reflection | Resolution: 1.5→25 Å / Num. obs: 51327 / % possible obs: 99.5 % / Redundancy: 7.7 % / Net I/σ(I): 32.5 |
-Processing
Software |
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Refinement | Method to determine structure: MOLECULAR REPLACEMENT Starting model: 4XZB Resolution: 1.5→25 Å / Cor.coef. Fo:Fc: 0.963 / Cor.coef. Fo:Fc free: 0.946 / SU B: 1.224 / SU ML: 0.047 / Cross valid method: THROUGHOUT / ESU R: 0.077 / ESU R Free: 0.079 / Stereochemistry target values: MAXIMUM LIKELIHOOD / Details: HYDROGENS HAVE BEEN ADDED IN THE RIDING POSITIONS
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Solvent computation | Ion probe radii: 0.8 Å / Shrinkage radii: 0.8 Å / VDW probe radii: 1.2 Å / Solvent model: MASK | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Displacement parameters | Biso mean: 16.29 Å2
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Refinement step | Cycle: 1 / Resolution: 1.5→25 Å
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Refine LS restraints |
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