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- PDB-4x2y: Crystal structure of a chimeric Murine Norovirus NS6 protease (in... -
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Basic information
Entry | Database: PDB / ID: 4x2y | ||||||
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Title | Crystal structure of a chimeric Murine Norovirus NS6 protease (inactive C139A mutant) in which the P4-P4 prime residues of the cleavage junction in the extended C-terminus have been replaced by the corresponding residues from the NS2-3 junction. | ||||||
![]() | NS6 Protease,NS6 Protease | ||||||
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Function / homology | ![]() ![]() ![]() ![]() ![]() ![]() ![]() ![]() ![]() ![]() ![]() ![]() ![]() ![]() Similarity search - Function | ||||||
Biological species | ![]() ![]() ![]() | ||||||
Method | ![]() ![]() | ||||||
![]() | Leen, E.N. / Pfeil, M.-P. / Fernandes, H. / Curry, S. | ||||||
![]() | ![]() Title: Structure determination of Murine Norovirus NS6 proteases with C-terminal extensions designed to probe protease-substrate interactions. Authors: Fernandes, H. / Leen, E.N. / Cromwell, H. / Pfeil, M.P. / Curry, S. #1: ![]() Title: Structure of a murine norovirus NS6 protease-product complex revealed by adventitious crystallisation. Authors: Leen, E.N. / Baeza, G. / Curry, S. | ||||||
History |
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Structure visualization
Structure viewer | Molecule: ![]() ![]() |
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Downloads & links
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PDBx/mmCIF format | ![]() | 76.9 KB | Display | ![]() |
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PDB format | ![]() | 56.6 KB | Display | ![]() |
PDBx/mmJSON format | ![]() | Tree view | ![]() | |
Others | ![]() |
-Validation report
Arichive directory | ![]() ![]() | HTTPS FTP |
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-Related structure data
Related structure data | ![]() 4x2vC ![]() 4x2wC ![]() 4x2xC ![]() 4ashS S: Starting model for refinement C: citing same article ( |
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Similar structure data |
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Links
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Assembly
Deposited unit | ![]()
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2 | ![]()
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Unit cell |
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Components
#1: Protein | Mass: 18824.516 Da / Num. of mol.: 2 / Fragment: UNP residues 998-1173,UNP residues 338-340 / Mutation: C139A,C139A Source method: isolated from a genetically manipulated source Source: (gene. exp.) ![]() ![]() ![]() ![]() ![]() ![]() #2: Water | ChemComp-HOH / | ![]() |
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-Experimental details
-Experiment
Experiment | Method: ![]() |
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Sample preparation
Crystal | Density Matthews: 2.13 Å3/Da / Density % sol: 42.26 % |
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Crystal grow![]() | Temperature: 291.15 K / Method: vapor diffusion, sitting drop Details: 0.2 M KSCN, 0.1 M Bis-Tris propane pH 6.5-7.5, 20% w/v PEG 3350, cryo 30% (v/v) PEG 3350 PH range: 6.5 - 7.5 |
-Data collection
Diffraction | Mean temperature: 100 K |
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Diffraction source | Source: ![]() |
Detector | Type: RIGAKU SATURN 944+ / Detector: CCD / Date: Apr 23, 2013 |
Radiation | Protocol: SINGLE WAVELENGTH / Monochromatic (M) / Laue (L): M / Scattering type: x-ray |
Radiation wavelength | Wavelength![]() |
Reflection | Resolution: 2.417→19.273 Å / Num. obs: 10817 / % possible obs: 91.14 % / Redundancy: 1.8 % / Rmerge(I) obs: 0.093 / Net I/σ(I): 6.7 |
Reflection shell | Resolution: 2.417→2.503 Å / Redundancy: 1.8 % / Rmerge(I) obs: 0.271 / Mean I/σ(I) obs: 2.31 / % possible all: 66.47 |
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Processing
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Refinement | Method to determine structure![]() ![]() Starting model: 4ASH Resolution: 2.417→19.273 Å / FOM work R set: 0.8164 / SU ML: 0.25 / Cross valid method: FREE R-VALUE / σ(F): 1.97 / Phase error: 25.76 / Stereochemistry target values: ML
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Solvent computation | Shrinkage radii: 0.9 Å / VDW probe radii: 1.11 Å / Solvent model: FLAT BULK SOLVENT MODEL | |||||||||||||||||||||||||||||||||||
Displacement parameters | Biso max: 54.93 Å2 / Biso mean: 28.3 Å2 / Biso min: 20.56 Å2 | |||||||||||||||||||||||||||||||||||
Refinement step | Cycle: final / Resolution: 2.417→19.273 Å
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Refine LS restraints |
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LS refinement shell | Refine-ID: X-RAY DIFFRACTION / Total num. of bins used: 4
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