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Open data
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Basic information
Entry | Database: PDB / ID: 4wfe | ||||||
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Title | Human TRAAK K+ channel in a K+ bound conductive conformation | ||||||
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![]() | METAL TRANSPORT / ![]() ![]() | ||||||
Function / homology | ![]() mechanosensitive potassium channel activity / TWIK related potassium channel (TREK) / detection of mechanical stimulus involved in sensory perception of touch / sensory perception of temperature stimulus / temperature-gated cation channel activity / Phase 4 - resting membrane potential / ![]() ![]() ![]() ![]() ![]() Similarity search - Function | ||||||
Biological species | ![]() ![]() ![]() ![]() ![]() | ||||||
Method | ![]() ![]() ![]() | ||||||
![]() | Brohawn, S.G. / MacKinnon, R. | ||||||
![]() | ![]() Title: Physical mechanism for gating and mechanosensitivity of the human TRAAK K+ channel. Authors: Brohawn, S.G. / Campbell, E.B. / MacKinnon, R. | ||||||
History |
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Structure visualization
Structure viewer | Molecule: ![]() ![]() |
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Downloads & links
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Download
PDBx/mmCIF format | ![]() | 545.3 KB | Display | ![]() |
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PDB format | ![]() | 451.5 KB | Display | ![]() |
PDBx/mmJSON format | ![]() | Tree view | ![]() | |
Others | ![]() |
-Validation report
Arichive directory | ![]() ![]() | HTTPS FTP |
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-Related structure data
Related structure data | ![]() 4wffC ![]() 4wfgC ![]() 4wfhC ![]() 4i9wS C: citing same article ( S: Starting model for refinement |
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Similar structure data |
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Links
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Assembly
Deposited unit | ![]()
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Unit cell |
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Noncrystallographic symmetry (NCS) | NCS domain:
NCS domain segments: Component-ID: 0 / Refine code: 0
NCS ensembles :
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Components
-Protein , 1 types, 2 molecules AB
#1: Protein | Mass: 32569.650 Da / Num. of mol.: 2 / Mutation: N104Q, N108Q Source method: isolated from a genetically manipulated source Source: (gene. exp.) ![]() ![]() ![]() ![]() |
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-Antibody , 2 types, 4 molecules DFEG
#2: Antibody | Mass: 23038.404 Da / Num. of mol.: 2 Source method: isolated from a genetically manipulated source Source: (gene. exp.) ![]() ![]() ![]() ![]() ![]() ![]() #3: Antibody | Mass: 23474.381 Da / Num. of mol.: 2 Source method: isolated from a genetically manipulated source Source: (gene. exp.) ![]() ![]() ![]() ![]() ![]() ![]() |
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-Non-polymers , 3 types, 200 molecules ![](data/chem/img/K.gif)
![](data/chem/img/CA.gif)
![](data/chem/img/HOH.gif)
![](data/chem/img/CA.gif)
![](data/chem/img/HOH.gif)
#4: Chemical | ChemComp-K / #5: Chemical | #6: Water | ChemComp-HOH / | ![]() |
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-Experimental details
-Experiment
Experiment | Method: ![]() |
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Sample preparation
Crystal | Density Matthews: 3.41 Å3/Da / Density % sol: 63.96 % |
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Crystal grow![]() | Temperature: 277 K / Method: vapor diffusion, hanging drop / pH: 8.8 Details: 50 mM Tris pH 8.8, 200 mM CaCl2, 27-30% (vol/vol) PEG400, 4% (vol/vol) polypropylene glycol PH range: 8-9 |
-Data collection
Diffraction | Mean temperature: 100 K |
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Diffraction source | Source: ![]() ![]() ![]() |
Detector | Type: MARMOSAIC 300 mm CCD / Detector: CCD / Date: Nov 7, 2012 |
Radiation | Protocol: SINGLE WAVELENGTH / Monochromatic (M) / Laue (L): M / Scattering type: x-ray |
Radiation wavelength | Wavelength![]() |
Reflection | Resolution: 2.5→48.2 Å / Num. obs: 69456 / % possible obs: 99.6 % / Redundancy: 3.8 % / Rsym value: 0.063 / Net I/σ(I): 22.6 |
Reflection shell | Resolution: 2.5→2.54 Å / Redundancy: 3.7 % / Mean I/σ(I) obs: 0.9 / % possible all: 99.8 |
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Processing
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Refinement | Method to determine structure![]() ![]() Starting model: 4I9W Resolution: 2.5→48.2 Å / Cor.coef. Fo:Fc: 0.955 / Cor.coef. Fo:Fc free: 0.942 / SU B: 24.206 / SU ML: 0.241 / Cross valid method: THROUGHOUT / ESU R: 0.317 / ESU R Free: 0.233 / Stereochemistry target values: MAXIMUM LIKELIHOOD / Details: HYDROGENS HAVE BEEN ADDED IN THE RIDING POSITIONS
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Solvent computation | Ion probe radii: 0.8 Å / Shrinkage radii: 0.8 Å / VDW probe radii: 1.2 Å / Solvent model: MASK | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Displacement parameters | Biso mean: 89.526 Å2
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Refinement step | Cycle: 1 / Resolution: 2.5→48.2 Å
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Refine LS restraints |
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