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- PDB-4w6f: Crystal Structure of Full-Length Split GFP Mutant K26C Disulfide ... -
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Open data
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Basic information
Entry | Database: PDB / ID: 4w6f | ||||||
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Title | Crystal Structure of Full-Length Split GFP Mutant K26C Disulfide Dimer, P 32 2 1 Space Group, Form 2 | ||||||
![]() | fluorescent protein D21H/K26C![]() | ||||||
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Function / homology | ![]() ![]() ![]() ![]() ![]() | ||||||
Biological species | synthetic construct (others) | ||||||
Method | ![]() ![]() ![]() | ||||||
![]() | Leibly, D.J. / Waldo, G.S. / Yeates, T.O. | ||||||
Funding support | ![]()
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![]() | ![]() Title: A Suite of Engineered GFP Molecules for Oligomeric Scaffolding. Authors: Leibly, D.J. / Arbing, M.A. / Pashkov, I. / DeVore, N. / Waldo, G.S. / Terwilliger, T.C. / Yeates, T.O. | ||||||
History |
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Structure visualization
Structure viewer | Molecule: ![]() ![]() |
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Downloads & links
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Download
PDBx/mmCIF format | ![]() | 194.2 KB | Display | ![]() |
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PDB format | ![]() | 156.9 KB | Display | ![]() |
PDBx/mmJSON format | ![]() | Tree view | ![]() | |
Others | ![]() |
-Validation report
Arichive directory | ![]() ![]() | HTTPS FTP |
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-Related structure data
Related structure data | ![]() 4w69C ![]() 4w6aC ![]() 4w6bC ![]() 4w6cC ![]() 4w6dC ![]() 4w6gC ![]() 4w6hC ![]() 4w6iC ![]() 4w6jC ![]() 4w6kC ![]() 4w6lC ![]() 4w6mC ![]() 4w6nC ![]() 4w6oC ![]() 4w6pC ![]() 4w6rC ![]() 4w6sC ![]() 4w6tC ![]() 4w6uC ![]() 4w72C ![]() 4w73C ![]() 4w74C ![]() 4w75C ![]() 4w76C ![]() 4w77C ![]() 4w7aC ![]() 4w7cC ![]() 4w7dC ![]() 4w7eC ![]() 4w7fC ![]() 4w7rC ![]() 4w7xC ![]() 2b3pS C: citing same article ( S: Starting model for refinement |
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Similar structure data |
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Links
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Assembly
Deposited unit | ![]()
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Unit cell |
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Components
#1: Protein | ![]() Mass: 26085.398 Da / Num. of mol.: 2 Source method: isolated from a genetically manipulated source Source: (gene. exp.) synthetic construct (others) / Production host: ![]() ![]() ![]() #2: Chemical | ChemComp-IMD / ![]() #3: Chemical | ChemComp-NI / | ![]() |
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-Experimental details
-Experiment
Experiment | Method: ![]() |
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Sample preparation
Crystal | Density Matthews: 5.77 Å3/Da / Density % sol: 78.67 % |
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Crystal grow![]() | Temperature: 298 K / Method: vapor diffusion, hanging drop / pH: 8 / Details: 10% 2-propanol, 0.1M Imidazole pH 8.0, 5mM NiCl2 |
-Data collection
Diffraction | Mean temperature: 100 K |
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Diffraction source | Source: ![]() ![]() ![]() |
Detector | Type: DECTRIS PILATUS 6M-F / Detector: PIXEL / Date: Oct 31, 2013 |
Radiation | Protocol: SINGLE WAVELENGTH / Monochromatic (M) / Laue (L): M / Scattering type: x-ray |
Radiation wavelength | Wavelength![]() |
Reflection | Resolution: 2.7→84.34 Å / Num. obs: 33538 / % possible obs: 100 % / Redundancy: 10 % / Rsym value: 0.3045 / Net I/σ(I): 5.64 |
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Processing
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Refinement | Method to determine structure![]() ![]() Starting model: 2B3P Resolution: 2.7→84.34 Å / SU ML: 0.31 / Cross valid method: FREE R-VALUE / σ(F): 1.38 / Phase error: 23.02 / Stereochemistry target values: ML
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Solvent computation | Shrinkage radii: 0.9 Å / VDW probe radii: 1.11 Å / Solvent model: FLAT BULK SOLVENT MODEL | |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Refinement step | Cycle: LAST / Resolution: 2.7→84.34 Å
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Refine LS restraints |
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LS refinement shell |
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Refinement TLS params. | Method: refined / Refine-ID: X-RAY DIFFRACTION
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Refinement TLS group |
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