+Open data
-Basic information
Entry | Database: PDB / ID: 4uuc | ||||||
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Title | Crystal structure of human ASB11 ankyrin repeat domain | ||||||
Components | ANKYRIN REPEAT AND SOCS BOX PROTEIN 11 | ||||||
Keywords | SIGNALING PROTEIN | ||||||
Function / homology | Function and homology information post-translational protein modification / positive regulation of protein catabolic process / Antigen processing: Ubiquitination & Proteasome degradation / Neddylation / protein ubiquitination / intracellular signal transduction / endoplasmic reticulum / cytosol Similarity search - Function | ||||||
Biological species | HOMO SAPIENS (human) | ||||||
Method | X-RAY DIFFRACTION / SYNCHROTRON / MOLECULAR REPLACEMENT / Resolution: 1.8 Å | ||||||
Authors | Pinkas, D.M. / Sanvitale, C. / Kragh Nielsen, T. / Guo, K. / Sorrell, F. / Berridge, G. / Ayinampudi, V. / Wang, D. / Newman, J.A. / Tallant, C. ...Pinkas, D.M. / Sanvitale, C. / Kragh Nielsen, T. / Guo, K. / Sorrell, F. / Berridge, G. / Ayinampudi, V. / Wang, D. / Newman, J.A. / Tallant, C. / Chaikuad, A. / Canning, P. / Kopec, J. / Krojer, T. / Vollmar, M. / Allerston, C.K. / Chalk, R. / Burgess-Brown, N. / von Delft, F. / Arrowsmith, C.H. / Edwards, A. / Bountra, C. / Bullock, A. | ||||||
Citation | Journal: To be Published Title: Crystal Structure of Human Asb11 Ankyrin Repeat Domain Authors: Pinkas, D.M. / Sanvitale, C. / Kragh Nielsen, T. / Guo, K. / Sorrell, F. / Berridge, G. / Ayinampudi, V. / Wang, D. / Newman, J.A. / Tallant, C. / Chaikuad, A. / Canning, P. / Kopec, J. / ...Authors: Pinkas, D.M. / Sanvitale, C. / Kragh Nielsen, T. / Guo, K. / Sorrell, F. / Berridge, G. / Ayinampudi, V. / Wang, D. / Newman, J.A. / Tallant, C. / Chaikuad, A. / Canning, P. / Kopec, J. / Krojer, T. / Vollmar, M. / Allerston, C.K. / Chalk, R. / Burgess-Brown, N. / von Delft, F. / Arrowsmith, C.H. / Edwards, A. / Bountra, C. / Bullock, A. | ||||||
History |
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-Structure visualization
Structure viewer | Molecule: MolmilJmol/JSmol |
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-Downloads & links
-Download
PDBx/mmCIF format | 4uuc.cif.gz | 92.9 KB | Display | PDBx/mmCIF format |
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PDB format | pdb4uuc.ent.gz | 71.8 KB | Display | PDB format |
PDBx/mmJSON format | 4uuc.json.gz | Tree view | PDBx/mmJSON format | |
Others | Other downloads |
-Validation report
Arichive directory | https://data.pdbj.org/pub/pdb/validation_reports/uu/4uuc ftp://data.pdbj.org/pub/pdb/validation_reports/uu/4uuc | HTTPS FTP |
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-Related structure data
Related structure data | 3zkjS S: Starting model for refinement |
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Similar structure data |
-Links
-Assembly
Deposited unit |
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1 |
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Unit cell |
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-Components
#1: Protein | Mass: 23998.590 Da / Num. of mol.: 1 / Fragment: ANKYRIN REPEAT DOMAIN, RESIDUES 64-287 Source method: isolated from a genetically manipulated source Source: (gene. exp.) HOMO SAPIENS (human) / Production host: ESCHERICHIA COLI (E. coli) / Strain (production host): BL21 / References: UniProt: Q8WXH4 |
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#2: Water | ChemComp-HOH / |
-Experimental details
-Experiment
Experiment | Method: X-RAY DIFFRACTION / Number of used crystals: 1 |
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-Sample preparation
Crystal | Density Matthews: 2.6 Å3/Da / Density % sol: 52.72 % / Description: NONE |
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Crystal grow | pH: 5.5 Details: 0.2M AMMONIUM ACETATE, 25% PEG3350, 0.1M BIS-TRIS PH 5.5 |
-Data collection
Diffraction | Mean temperature: 100 K |
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Diffraction source | Source: SYNCHROTRON / Site: Diamond / Beamline: I03 / Wavelength: 0.97625 |
Detector | Type: DECTRIS PILATUS 6M / Detector: PIXEL / Date: Jul 6, 2014 |
Radiation | Protocol: SINGLE WAVELENGTH / Monochromatic (M) / Laue (L): M / Scattering type: x-ray |
Radiation wavelength | Wavelength: 0.97625 Å / Relative weight: 1 |
Reflection | Resolution: 1.8→89.6 Å / Num. obs: 23795 / % possible obs: 99.7 % / Observed criterion σ(I): -3 / Redundancy: 5.9 % / Biso Wilson estimate: 22.8 Å2 / Rmerge(I) obs: 0.1 / Net I/σ(I): 11.1 |
Reflection shell | Resolution: 1.8→1.84 Å / Redundancy: 3 % / Rmerge(I) obs: 0.73 / Mean I/σ(I) obs: 2.3 / % possible all: 98.7 |
-Processing
Software |
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Refinement | Method to determine structure: MOLECULAR REPLACEMENT Starting model: PDB ENTRY 3ZKJ Resolution: 1.8→52.281 Å / SU ML: 0.19 / σ(F): 1.35 / Phase error: 18.89 / Stereochemistry target values: ML
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Solvent computation | Shrinkage radii: 0.9 Å / VDW probe radii: 1.11 Å / Solvent model: FLAT BULK SOLVENT MODEL | |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Displacement parameters | Biso mean: 26.86 Å2 | |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Refinement step | Cycle: LAST / Resolution: 1.8→52.281 Å
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Refine LS restraints |
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LS refinement shell |
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