温度: 293 K / 手法: 蒸気拡散法, シッティングドロップ法 詳細: Obtained by equilibrating a 2 or 4 uL drop of 1:1 protein: reservoir against 700 uL reservoir containing 0.7 M NaH2PO4, 0.9 M K2HPO4. Cryoprotection was achieved by carefully adding equal ...詳細: Obtained by equilibrating a 2 or 4 uL drop of 1:1 protein: reservoir against 700 uL reservoir containing 0.7 M NaH2PO4, 0.9 M K2HPO4. Cryoprotection was achieved by carefully adding equal drop volume of mother liquor containing 50% glycerol. Crystals were then transferred into mother liquor containing 30% glycerol and flash frozen in liquid nitrogen. All crystal growth and manipulation was performed anaerobically.
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データ収集
回折
平均測定温度: 100 K
放射光源
由来: シンクロトロン / サイト: ALS / ビームライン: 8.2.1 / 波長: 1.11 Å
検出器
タイプ: ADSC QUANTUM 315r / 検出器: CCD / 日付: 2012年7月7日
放射
プロトコル: SINGLE WAVELENGTH / 単色(M)・ラウエ(L): M / 散乱光タイプ: x-ray
放射波長
波長: 1.11 Å / 相対比: 1
反射
解像度: 1.65→33.818 Å / Num. all: 45550 / Num. obs: 45550 / % possible obs: 98.46 % / 冗長度: 3.9 % / Rmerge(I) obs: 0.029 / Net I/σ(I): 14.4
反射 シェル
解像度: 1.65→1.74 Å / 冗長度: 3.3 % / Rmerge(I) obs: 0.487 / Mean I/σ(I) obs: 1.7 / % possible all: 99.5