+Open data
-Basic information
Entry | Database: PDB / ID: 4u6h | ||||||||||||
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Title | Vaccinia L1/M12B9-Fab complex | ||||||||||||
Components |
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Keywords | IMMUNE SYSTEM / IgG2a / Fab / heavy chain light chain | ||||||||||||
Function / homology | Function and homology information symbiont entry into host cell / viral envelope / virion attachment to host cell / virion membrane / membrane Similarity search - Function | ||||||||||||
Biological species | Vaccinia virus Mus musculus (house mouse) | ||||||||||||
Method | X-RAY DIFFRACTION / SYNCHROTRON / MOLECULAR REPLACEMENT / Resolution: 3.1 Å | ||||||||||||
Authors | Matho, M.H. / Schlossman, A. / Zajonc, D.M. | ||||||||||||
Funding support | United States, 3items
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Citation | Journal: J.Virol. / Year: 2014 Title: Potent neutralization of vaccinia virus by divergent murine antibodies targeting a common site of vulnerability in l1 protein. Authors: Kaever, T. / Meng, X. / Matho, M.H. / Schlossman, A. / Li, S. / Sela-Culang, I. / Ofran, Y. / Buller, M. / Crump, R.W. / Parker, S. / Frazier, A. / Crotty, S. / Zajonc, D.M. / Peters, B. / Xiang, Y. | ||||||||||||
History |
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-Structure visualization
Structure viewer | Molecule: MolmilJmol/JSmol |
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-Downloads & links
-Download
PDBx/mmCIF format | 4u6h.cif.gz | 235.4 KB | Display | PDBx/mmCIF format |
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PDB format | pdb4u6h.ent.gz | 189.6 KB | Display | PDB format |
PDBx/mmJSON format | 4u6h.json.gz | Tree view | PDBx/mmJSON format | |
Others | Other downloads |
-Validation report
Arichive directory | https://data.pdbj.org/pub/pdb/validation_reports/u6/4u6h ftp://data.pdbj.org/pub/pdb/validation_reports/u6/4u6h | HTTPS FTP |
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-Related structure data
-Links
-Assembly
Deposited unit |
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1 |
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2 |
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Unit cell |
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-Components
#1: Antibody | Mass: 23757.602 Da / Num. of mol.: 2 / Source method: isolated from a natural source / Source: (natural) Mus musculus (house mouse) / Cell line: splenocyte / Organ: spleen / Plasmid details: fusion with mouse myeloma SP2/0 cells / Strain: Balb/c #2: Antibody | Mass: 24105.637 Da / Num. of mol.: 2 / Source method: isolated from a natural source / Source: (natural) Mus musculus (house mouse) / Cell line: Splenocyte / Organ: Spleen Plasmid details: splenocytes fused with the mouse myeloma SP2/0 cells Strain: Balb/c #3: Protein | Mass: 19535.943 Da / Num. of mol.: 2 Source method: isolated from a genetically manipulated source Source: (gene. exp.) Vaccinia virus / Strain: Western Reserve / Gene: VACWR088, L1R / Plasmid: pML1 / Details (production host): same as for entry 1YPY / Production host: Escherichia coli (E. coli) / References: UniProt: P07612 |
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-Experimental details
-Experiment
Experiment | Method: X-RAY DIFFRACTION / Number of used crystals: 1 |
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-Sample preparation
Crystal | Density Matthews: 2.6 Å3/Da / Density % sol: 52.69 % |
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Crystal grow | Temperature: 293 K / Method: vapor diffusion, hanging drop / pH: 7 Details: Initial crystallization experiments were carried out by sitting-drop vapor diffusion in a 96-well format, using a Phoenix liquid-handling robot with a panel of commercial sparse-matrix ...Details: Initial crystallization experiments were carried out by sitting-drop vapor diffusion in a 96-well format, using a Phoenix liquid-handling robot with a panel of commercial sparse-matrix screens (PEG/Ion 1 and 2 from Hampton Research, Wizard 2 from Emerald Biosciences, JCSG Plus Suite from Qiagen, and JBScreen 6 from Jena BioScience). Quality diffracting crystals of L1/M12B9-Fab complex were obtained at RT by mixing 0.5ul of protein solution at 9.5 mg/ml with 0.5ul of precipitant [100 mM Tris, pH 7.0, 20% (w/v) polyethylene glycol (PEG) 3000, and 200 mM Ca(OAc)] and seeding with initial crystals obtained at 6.5 mg/ml. Crystal were flash-frozen at 100K in mother liquor containing 20 % glycerol. |
-Data collection
Diffraction | Mean temperature: 100 K |
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Diffraction source | Source: SYNCHROTRON / Site: SSRL / Beamline: BL11-1 / Wavelength: 0.97945 Å |
Detector | Type: DECTRIS PILATUS 6M / Detector: PIXEL / Date: May 29, 2013 |
Radiation | Monochromator: Side scattering bent cube-root I-beam single crystal; asymmetric cut 4.965 degs Protocol: SINGLE WAVELENGTH / Monochromatic (M) / Laue (L): M / Scattering type: x-ray |
Radiation wavelength | Wavelength: 0.97945 Å / Relative weight: 1 |
Reflection | Resolution: 3.1→47.67 Å / Num. obs: 27308 / % possible obs: 99.9 % / Observed criterion σ(I): 1.7 / Redundancy: 9.2 % / Rmerge(I) obs: 0.2 / Rsym value: 0.077 / Net I/σ(I): 9.7 |
-Processing
Software | Name: REFMAC / Version: 5.7.0029 / Classification: refinement | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
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Refinement | Method to determine structure: MOLECULAR REPLACEMENT Starting model: 1YPY,1SY6 Resolution: 3.1→47.21 Å / Cor.coef. Fo:Fc: 0.921 / Cor.coef. Fo:Fc free: 0.871 / SU B: 23.379 / SU ML: 0.395 / Cross valid method: FREE R-VALUE / ESU R Free: 0.498 / Stereochemistry target values: MAXIMUM LIKELIHOOD / Details: HYDROGENS HAVE BEEN ADDED IN THE RIDING POSITIONS
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Solvent computation | Ion probe radii: 0.8 Å / Shrinkage radii: 0.8 Å / VDW probe radii: 1.2 Å / Solvent model: MASK | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Displacement parameters | Biso mean: 64.52 Å2
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Refinement step | Cycle: 1 / Resolution: 3.1→47.21 Å
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