PDB-4tzg: Crystal structure of eCGP123, an extremely thermostable green flu...

Basic information

• Entry: Database: PDB / ID: 4tzg
• Title: Crystal structure of eCGP123, an extremely thermostable green fluorescent protein
• Components: Fluorescent Protein
• Keywords: FLUORESCENT PROTEIN / thermostable / engineered
• Function / homology: Green Fluorescent Protein / Green fluorescent protein / Beta Barrel / Mainly Beta
• Biological species: synthetic construct (others)
• Method: X-RAY DIFFRACTION / MOLECULAR REPLACEMENT / Resolution: 2.1 Å
• Authors: Close, D.W. / Don Paul, C. / Traore, D.A.K. / Wilce, M.C.J. / Prescott, M. / Bradbury, A.R.M.
• Citation: Journal: Proteins / Year: 2015; Title: Thermal green protein, an extremely stable, nonaggregating fluorescent protein created by structure-guided surface engineering.; Authors: Close, D.W. / Paul, C.D. / Langan, P.S. / Wilce, M.C. / Traore, D.A. / Halfmann, R. / Rocha, R.C. / Waldo, G.S. / Payne, R.J. / Rucker, J.B. / Prescott, M. / Bradbury, A.R.

History

Deposition	Jul 10, 2014	Deposition site: RCSB / Processing site: RCSB
Revision 1.0	Oct 22, 2014	Provider: repository / Type: Initial release
Revision 1.1	Feb 4, 2015	Group: Derived calculations
Revision 1.2	Jun 3, 2015	Group: Database references
Revision 1.3	Feb 17, 2016	Group: Database references
Revision 1.4	Jun 1, 2016	Group: Data collection
Revision 1.5	Nov 22, 2017	Group: Derived calculations / Refinement description / Category: pdbx_struct_oper_list / software / Item: _pdbx_struct_oper_list.symmetry_operation
Revision 1.6	Sep 27, 2023	Group: Data collection / Database references / Refinement description Category: chem_comp_atom / chem_comp_bond / database_2 / pdbx_initial_refinement_model Item: _database_2.pdbx_DOI / _database_2.pdbx_database_accession
Revision 2.0	Nov 15, 2023	Group: Atomic model / Data collection / Derived calculations Category: atom_site / atom_site_anisotrop / chem_comp_atom / chem_comp_bond / pdbx_validate_rmsd_angle / struct_conn Item: _atom_site.auth_atom_id / _atom_site.label_atom_id / _atom_site_anisotrop.pdbx_auth_atom_id / _atom_site_anisotrop.pdbx_label_atom_id / _chem_comp_atom.atom_id / _chem_comp_bond.atom_id_1 / _chem_comp_bond.atom_id_2 / _struct_conn.ptnr1_label_atom_id / _struct_conn.ptnr2_label_atom_id

Assembly

Deposited unit

A: Fluorescent Protein

B: Fluorescent Protein

C: Fluorescent Protein

D: Fluorescent Protein

E: Fluorescent Protein

F: Fluorescent Protein

G: Fluorescent Protein

H: Fluorescent Protein

Summary:

• 226 kDa, 8 polymers

Component details:

	Theoretical mass	Number of molelcules
Total (without water)	225,993	8
Polymers	225,993	8
Non-polymers	0	0
Water	18,573	1031

1

A: Fluorescent Protein

Summary:

• defined by author&software
• 28.2 kDa, 1 polymers

Component details:

	Theoretical mass	Number of molelcules
Total (without water)	28,249	1
Polymers	28,249	1
Non-polymers	0	0
Water	18	1

Symmetry operations:

Type	Name	Symmetry operation	Number
identity operation	1_555	x,y,z	1

Calculated values:

Method	PISA

2

B: Fluorescent Protein

Summary:

• defined by author&software
• 28.2 kDa, 1 polymers

Component details:

	Theoretical mass	Number of molelcules
Total (without water)	28,249	1
Polymers	28,249	1
Non-polymers	0	0
Water	18	1

Symmetry operations:

Type	Name	Symmetry operation	Number
identity operation	1_555	x,y,z	1

Calculated values:

Method	PISA

3

C: Fluorescent Protein

Summary:

• defined by author&software
• 28.2 kDa, 1 polymers

Component details:

	Theoretical mass	Number of molelcules
Total (without water)	28,249	1
Polymers	28,249	1
Non-polymers	0	0
Water	18	1

Symmetry operations:

Type	Name	Symmetry operation	Number
identity operation	1_555	x,y,z	1

Calculated values:

Method	PISA

4

D: Fluorescent Protein

Summary:

• defined by author&software
• 28.2 kDa, 1 polymers

Component details:

	Theoretical mass	Number of molelcules
Total (without water)	28,249	1
Polymers	28,249	1
Non-polymers	0	0
Water	18	1

Symmetry operations:

Type	Name	Symmetry operation	Number
identity operation	1_555	x,y,z	1

Calculated values:

Method	PISA

5

E: Fluorescent Protein

Summary:

• defined by author&software
• 28.2 kDa, 1 polymers

Component details:

	Theoretical mass	Number of molelcules
Total (without water)	28,249	1
Polymers	28,249	1
Non-polymers	0	0
Water	18	1

Symmetry operations:

Type	Name	Symmetry operation	Number
identity operation	1_555	x,y,z	1

Calculated values:

Method	PISA

6

F: Fluorescent Protein

Summary:

• defined by author&software
• 28.2 kDa, 1 polymers

Component details:

	Theoretical mass	Number of molelcules
Total (without water)	28,249	1
Polymers	28,249	1
Non-polymers	0	0
Water	18	1

Symmetry operations:

Type	Name	Symmetry operation	Number
identity operation	1_555	x,y,z	1

Calculated values:

Method	PISA

7

G: Fluorescent Protein

Summary:

• defined by author&software
• 28.2 kDa, 1 polymers

Component details:

	Theoretical mass	Number of molelcules
Total (without water)	28,249	1
Polymers	28,249	1
Non-polymers	0	0
Water	18	1

Symmetry operations:

Type	Name	Symmetry operation	Number
identity operation	1_555	x,y,z	1

Calculated values:

Method	PISA

8

H: Fluorescent Protein

Summary:

• defined by author&software
• 28.2 kDa, 1 polymers

Component details:

	Theoretical mass	Number of molelcules
Total (without water)	28,249	1
Polymers	28,249	1
Non-polymers	0	0
Water	18	1

Symmetry operations:

Type	Name	Symmetry operation	Number
identity operation	1_555	x,y,z	1

Calculated values:

Method	PISA

Unit cell

Length a, b, c (Å)	74.6299, 75.3804, 84.5129
Angle α, β, γ (deg.)	90.9592, 89.8211, 104.031
Int Tables number	1
Space group name H-M	P1
Space group name Hall	P1
Symmetry operation	#1: x,y,z

Components

#1: Protein

A B C D E F G H
Fluorescent Protein /

Details:

Mass: 28249.162 Da / Num. of mol.: 8
Source method: isolated from a genetically manipulated source
Source: (gene. exp.) synthetic construct (others) / Plasmid: pETCK3 / Production host: Escherichia coli (E. coli) / Strain (production host): Nova Blue DE3

#2: Water

ChemComp-HOH / water / Water

Details:

Mass: 18.015 Da / Num. of mol.: 1031 / Source method: isolated from a natural source / Formula: H₂O

• Sequence details: CRQ is the chromophore that folds from the amino acids QYG, which are encoded in the original sequence. MGAHA represents N-terminal residues added when expressed from the plasmid. SGGGSGGGASGKPIPNPLLGLDSTHHHHHH are C-terminal SV5 and His Tags.

Experimental details

Experiment

• Experiment: Method: X-RAY DIFFRACTION

Sample preparation

• Crystal: Density Matthews: 2.26 ų/Da / Density % sol: 45.6 %
• Crystal grow: Temperature: 293 K / Method: vapor diffusion, hanging drop; Details: 0.2 M Tris-HCl pH 8.0, 22.5% (w/v) PEG 3350, 0.2 M MgCl2

Data collection

• Diffraction: Mean temperature: 100 K
• Diffraction source: Source: ROTATING ANODE / Type: RIGAKU RUH3R / Wavelength: 1.542 Å
• Detector: Type: RIGAKU RAXIS IV++ / Detector: IMAGE PLATE / Date: May 15, 2009
• Radiation: Protocol: SINGLE WAVELENGTH / Monochromatic (M) / Laue (L): M / Scattering type: x-ray
• Radiation wavelength: Wavelength: 1.542 Å / Relative weight: 1
• Reflection: Resolution: 2.1→29.85 Å / Num. obs: 98476 / % possible obs: 94.4 % / Redundancy: 2 % / B_iso Wilson estimate: 33.3936056739 Ų / Net I/σ(I): 9.6

Processing

Software

Name	Version	Classification
PHENIX	1.9_1685	refinement
MOSFLM	1.9_1685	data reduction
SCALA		data scaling
Coot		model building

Refinement

Method to determine structure: MOLECULAR REPLACEMENT
Starting model: 3ADF

3adf

Resolution: 2.1→29.85 Å / SU ML: 0.231082958389 / Cross valid method: FREE R-VALUE / σ(F): 1.96706026102 / Phase error: 22.3957155672

	Rfactor	Num. reflection	% reflection
Rfree	0.203299488364	4885	4.96256488922 %
Rwork	0.167453291166	93552	-
obs	0.16923286509	98437	94.4049639881 %

• Solvent computation: Shrinkage radii: 0.9 Å / VDW probe radii: 1.11 Å / Solvent model: FLAT BULK SOLVENT MODEL
• Displacement parameters: B_iso mean: 45.3200752535 Ų

Refinement step

Cycle: LAST / Resolution: 2.1→29.85 Å

	Protein	Nucleic acid	Ligand	Solvent	Total
Num. atoms	14341	0	0	1031	15372

Refine LS restraints

Refine-ID	Type	Dev ideal	Number
X-RAY DIFFRACTION	f_bond_d	0.00446055428172	14733
X-RAY DIFFRACTION	f_angle_d	0.928624660861	19838
X-RAY DIFFRACTION	f_chiral_restr	0.0348320968203	2012
X-RAY DIFFRACTION	f_plane_restr	0.003393443661	2557
X-RAY DIFFRACTION	f_dihedral_angle_d	13.1976346749	5658

LS refinement shell

Resolution (Å)	Rfactor Rfree	Num. reflection Rfree	Rfactor Rwork	Num. reflection Rwork	Refine-ID	% reflection obs (%)
2.1-2.1239	0.289090738803	161	0.245322703238	3047	X-RAY DIFFRACTION	91.266002845
2.1239-2.1488	0.277424412046	151	0.243911362089	3042	X-RAY DIFFRACTION	92.2565732447
2.1488-2.175	0.272196333853	150	0.23342478343	3017	X-RAY DIFFRACTION	91.5847310584
2.175-2.2026	0.258131067912	160	0.233335192655	3072	X-RAY DIFFRACTION	92.0797720798
2.2026-2.2315	0.262563778986	164	0.230901421079	3037	X-RAY DIFFRACTION	92.6483357453
2.2315-2.2621	0.244387720151	160	0.222574314135	3029	X-RAY DIFFRACTION	92.2476135377
2.2621-2.2944	0.273603934466	159	0.221817163831	3077	X-RAY DIFFRACTION	92.4835667334
2.2944-2.3286	0.252165491806	178	0.213097088896	3027	X-RAY DIFFRACTION	92.9524361949
2.3286-2.365	0.276692565152	168	0.215951260872	3072	X-RAY DIFFRACTION	93.1034482759
2.365-2.4038	0.254342738491	157	0.203991551338	3097	X-RAY DIFFRACTION	93.2378223496
2.4038-2.4452	0.223705005457	153	0.201651650104	3059	X-RAY DIFFRACTION	92.9398148148
2.4452-2.4896	0.223592729869	169	0.201852220696	3125	X-RAY DIFFRACTION	93.8461538462
2.4896-2.5375	0.246109655967	149	0.202892403529	3044	X-RAY DIFFRACTION	93.9393939394
2.5375-2.5893	0.262577630803	159	0.200805349846	3140	X-RAY DIFFRACTION	94.0153889997
2.5893-2.6455	0.239051592707	155	0.194414023579	3134	X-RAY DIFFRACTION	94.2136923518
2.6455-2.707	0.231118344748	162	0.193932072731	3087	X-RAY DIFFRACTION	94.2832269298
2.707-2.7747	0.246662917755	144	0.178767757255	3155	X-RAY DIFFRACTION	94.5814220183
2.7747-2.8497	0.229935895429	177	0.190063157647	3101	X-RAY DIFFRACTION	94.6851530907
2.8497-2.9334	0.259689891586	170	0.196475034658	3124	X-RAY DIFFRACTION	94.8459545062
2.9334-3.028	0.22540811831	183	0.189595129234	3158	X-RAY DIFFRACTION	95.1309794989
3.028-3.1361	0.236174893751	169	0.181794061269	3159	X-RAY DIFFRACTION	95.3581661891
3.1361-3.2616	0.206120125023	184	0.17855843035	3136	X-RAY DIFFRACTION	95.8429561201
3.2616-3.4098	0.216407064431	177	0.162046419951	3157	X-RAY DIFFRACTION	95.5300859599
3.4098-3.5893	0.182625343196	155	0.155120294409	3178	X-RAY DIFFRACTION	96.4130749205
3.5893-3.8138	0.165497675615	154	0.145539893333	3190	X-RAY DIFFRACTION	96.4522642054
3.8138-4.1075	0.165941686238	176	0.132540237866	3158	X-RAY DIFFRACTION	96.6937354988
4.1075-4.5196	0.157383429329	162	0.120097482773	3236	X-RAY DIFFRACTION	96.9748858447
4.5196-5.1707	0.132836386475	157	0.120246255004	3225	X-RAY DIFFRACTION	97.351755901
5.1707-6.5034	0.168913002979	151	0.138514743191	3241	X-RAY DIFFRACTION	97.7803401557
6.5034-29.85	0.180369354838	171	0.1553705804	3228	X-RAY DIFFRACTION	97.5602755454

Refinement TLS params.

Method: refined / Refine-ID: X-RAY DIFFRACTION

ID	L11 (°2)	L12 (°2)	L13 (°2)	L22 (°2)	L23 (°2)	L33 (°2)	S11 (Å °)	S12 (Å °)	S13 (Å °)	S21 (Å °)	S22 (Å °)	S23 (Å °)	S31 (Å °)	S32 (Å °)	S33 (Å °)	T11 (Å2)	T12 (Å2)	T13 (Å2)	T22 (Å2)	T23 (Å2)	T33 (Å2)	Origin x (Å)	Origin y (Å)	Origin z (Å)
1	3.47737381081	-1.0524852509	1.17413419774	1.61772643239	-0.830229794487	3.12768876162	0.0685206519544	-0.16793868834	-0.536827895283	-0.0498711548143	0.029080471646	0.286257335226	0.30146016396	-0.198634035305	-0.128661825602	0.227253433942	-0.0335139009428	0.060210753773	0.217568830779	-8.88859726924E-5	0.33839811057	-5.30599932089	8.87541625107	7.02068936153
2	0.922020757442	-0.381063742524	-0.0714203962147	1.46121340407	-0.193259072503	1.58053971959	0.0237184271289	-0.0397013287437	-0.0833124364935	-0.0231189272201	-0.00597722561323	0.184276610628	0.0111499246315	-0.0277234527382	-0.0337091693907	0.196568852699	-0.0201769077835	0.0307404902893	0.286677039686	-0.00420939764422	0.251310126009	-3.53934753308	18.5693732475	2.18124580113
3	2.46555789037	-0.361839114296	-0.206347811628	1.58697639356	0.117352506026	2.71526373543	0.0426962543506	-0.00470133561681	-0.276730857336	0.0296029051812	-0.0227576692209	-0.104331203841	0.186849111023	0.26018192081	-0.0109300824662	0.200992338991	0.022517591797	0.031517900787	0.229750928862	0.0418399473896	0.270841751697	3.74437994321	14.0005229064	5.96578852926
4	1.12236062062	0.0328510524795	0.61630172926	0.873935593426	-0.298246298314	5.76954412708	0.0990239382821	-0.00972275549224	-0.00701744592761	-0.0527357016563	-0.0668039704292	0.0862854562796	-0.216724868262	0.0390511212775	-0.0276401667678	0.224720674593	0.00687439867462	0.0243659559156	0.255307655607	0.00118510555615	0.301151675116	-2.76727572022	27.3846363545	2.7514305072
5	1.91106546203	0.915339857912	1.2194978467	1.78043822957	1.02334685115	4.01760729108	0.0749702495244	0.0309773044603	0.0885421690633	-0.00928074036188	0.0822901636124	-0.0675086148424	-0.369740082054	0.171536124454	-0.167812146637	0.230446225159	-0.015582708837	0.0202368443895	0.248234362303	0.0143078971466	0.270115539326	1.5511220621	26.6720060373	5.38651053917
6	1.66743992752	-0.294695927115	0.664334557469	2.0893521326	-0.955237656222	1.7643205389	-0.0533069059635	-0.165353454715	0.0550452763545	0.0926437479921	0.0533029133846	0.238162095346	-0.0477001398959	-0.199564620089	-0.0209849871258	0.24148584881	0.0170902513513	0.0664210870364	0.374808993457	0.0216839444717	0.31833487254	-9.18315611707	22.6753804405	5.11747821404
7	3.83483484224	0.52272802491	1.04900271163	1.4806230506	0.653138357124	1.53542736763	0.0730633658691	0.0240135271512	-0.888402845433	0.00309165129669	0.0530945956527	-0.338290388333	0.275668779385	0.06942856149	-0.156892021181	0.321626793289	0.020832967783	0.0484355055159	0.234908950934	0.0143309995346	0.412110644233	28.3807071304	5.6350175918	-4.96409424669
8	2.0605594267	0.32339451617	0.528071453455	1.43855156041	0.561671330248	2.59580315796	0.0269340240935	-0.0710214992446	-0.26299009531	0.016102483862	-0.0378743276518	-0.111793706506	0.161681775339	-0.132188610411	0.00216198390278	0.183377781799	0.00378976114161	0.0392865614487	0.187135547204	0.00561149222095	0.218354995631	24.7869080617	13.9975655791	-1.42881713071
9	1.19505714616	0.1937544725	0.663828872662	1.39636218659	0.68916872958	4.70305292755	-0.00336944064518	0.0322659795137	0.0068690665929	-0.16669390999	-0.0204857800688	-0.11105085869	-0.146246571653	0.140213618172	-0.00955975299899	0.199798746926	0.0211942538518	0.0303670797439	0.212815064767	-0.00539849764609	0.221227189832	26.9592289893	23.7504198209	-3.31584797678
10	2.25351426071	-0.435130252982	2.20066632036	1.78579084853	-0.638232547472	5.19192753388	0.0234784925169	0.0486032441339	0.013397717402	-0.204494637384	-0.0570164274042	-0.0468643958231	-0.277522328878	-0.163076185651	0.0300966553806	0.244045328794	0.0216921217705	0.00617704437818	0.210279749905	-0.004151710633	0.242295519019	24.6917866804	25.1856336853	-6.49410471418
11	4.5478570881	-0.891435445351	2.77624081709	0.570417249903	1.193536605	9.44543697184	-0.199492815011	-0.314483129815	-0.0841925291706	0.011354415803	0.0447003359262	0.068419145912	-0.736281565303	-0.431839514055	0.205508217247	0.223733992564	0.016074025268	0.0329540837944	0.192355938633	-0.00066861473169	0.225833504413	22.9201818082	23.3773828902	-0.913198000013
12	1.94654842729	0.318052309878	0.608380283419	1.56189630737	0.796078888816	2.15174235122	-0.126132130415	0.143113978079	-0.0872739665074	-0.183171506623	0.0108537435926	-0.237737887803	0.0117120522885	0.255100113617	0.0945941988797	0.204285042198	0.0193286984075	0.0573701945368	0.245414736726	0.0412118462029	0.268171110037	34.0296191091	18.7576494446	-3.5246803782
13	7.02050239994	0.571103986948	-1.97432916356	2.31549245899	-0.799292186064	3.21276088934	0.156168449207	-0.0357051016052	0.628005688897	0.0757967614465	-0.101862302928	0.199370676502	-0.417317939669	0.222225437344	-0.125007535992	0.32773691544	-0.0327285148072	-0.0714829356597	0.258670631152	0.00115345567373	0.324726106187	33.2052944805	32.8749058644	38.2493817192
14	1.25816885685	0.272914715437	-0.231382962689	1.45976498586	-0.315865131593	2.28271406884	0.00132886464672	0.00936529305162	0.119424226198	0.0525053162006	-0.0643694230219	0.0845720816118	-0.107209001473	0.126088992821	0.0726123693328	0.181118219534	-0.00926273205092	-0.038787990004	0.213513970406	0.000122757865134	0.213675845983	33.8170640942	25.7320862234	41.2723697412
15	8.72241894078	1.67745337626	3.66354622363	8.15409357193	1.87583210118	7.77250464403	-0.205832751864	-0.143148404297	-0.474234200194	-0.360183305035	0.115641322437	-0.777018544068	-0.0296079849924	0.766354907738	0.0979927647886	0.251092840754	0.021613032791	0.0608647772485	0.525947102896	0.0860852852185	0.323371642974	50.5583201557	19.428495016	26.6308000779
16	1.31750603555	0.120186515204	0.045931105136	1.07003784428	0.126179769232	4.76698912753	0.113646535213	0.0700804506738	-0.0258125704182	0.048997021608	-0.133928022662	0.0640074345646	0.298698296576	0.121717182013	-0.066157146021	0.213189525418	0.00634583075323	-0.0107067175201	0.225001904103	0.0120103262679	0.259322892533	34.6025069202	13.5854831014	41.3247407518
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53	2.11784359493	0.507268684278	-1.02045262174	1.98020636739	-0.357500198741	5.53913496584	0.177675403728	-0.0631976596394	0.0338650811664	0.515424911569	0.0336807010372	0.041473674885	-0.170508526766	-0.168346139512	-0.162128935008	0.375364399306	0.0201314976463	0.0122476604875	0.284558502664	0.0334728148713	0.262320479509	-7.5897306753	3.45192311172	47.031941088
54	0.571532977974	0.0505445267545	-0.076484970025	2.37491670038	-2.71659956254	2.0093322118	-0.201040869377	0.0122784877734	-0.00225294644751	0.246667522326	-0.162401940884	-0.0369959072954	0.126576399167	-0.117248398183	0.32434288613	0.263849052208	0.0337913621658	-0.00214717360528	0.413210640406	0.00067105894285	0.317647898776	-9.5635534701	3.6376624227	44.643246861
55	2.38764021205	-0.609990149326	0.715037322095	4.17530924308	-3.30453180378	8.85788064258	-0.0845596040095	-0.0712048772362	-0.166687587806	0.217870358183	-0.0255423691952	0.0933174607198	0.314513327235	-0.367691100291	0.167846998165	0.311334472877	-0.0470695213094	0.0290471041556	0.247177202188	-0.0410740986024	0.236672542778	-11.4168562389	2.18396095672	42.0844440972
56	1.88879511439	0.671126836519	0.0350434502834	2.9497682269	0.584844176235	1.82241896465	0.0887572408385	-0.0158926903771	-0.154473488442	0.443110204497	-0.147307337166	-0.470266224464	0.406209785458	0.166539385849	0.0573007104939	0.299135539638	0.0440909049793	-0.0412650733593	0.337083274794	0.0423774331629	0.335652281245	-0.66519955342	-1.24152865624	46.8684744115

Refinement TLS group

ID	Refine-ID	Refine TLS-ID	Selection details
1	X-RAY DIFFRACTION	1	chain A and (resid 3:46 )
2	X-RAY DIFFRACTION	2	chain A and (resid 47:99 )
3	X-RAY DIFFRACTION	3	chain A and (resid 100:135 )
4	X-RAY DIFFRACTION	4	chain A and (resid 136:151 )
5	X-RAY DIFFRACTION	5	chain A and (resid 152:179 )
6	X-RAY DIFFRACTION	6	chain A and (resid 180:223 )
7	X-RAY DIFFRACTION	7	chain B and (resid 2:46 )
8	X-RAY DIFFRACTION	8	chain B and (resid 47:133 )
9	X-RAY DIFFRACTION	9	chain B and (resid 134:151 )
10	X-RAY DIFFRACTION	10	chain B and (resid 152:167 )
11	X-RAY DIFFRACTION	11	chain B and (resid 168:179 )
12	X-RAY DIFFRACTION	12	chain B and (resid 180:223 )
13	X-RAY DIFFRACTION	13	chain C and (resid 2:32 )
14	X-RAY DIFFRACTION	14	chain C and (resid 33:123 )
15	X-RAY DIFFRACTION	15	chain C and (resid 124:135 )
16	X-RAY DIFFRACTION	16	chain C and (resid 136:151 )
17	X-RAY DIFFRACTION	17	chain C and (resid 152:179 )
18	X-RAY DIFFRACTION	18	chain C and (resid 180:200 )
19	X-RAY DIFFRACTION	19	chain C and (resid 201:223 )
20	X-RAY DIFFRACTION	20	chain D and (resid 2:20 )
21	X-RAY DIFFRACTION	21	chain D and (resid 21:99 )
22	X-RAY DIFFRACTION	22	chain D and (resid 100:123 )
23	X-RAY DIFFRACTION	23	chain D and (resid 124:135 )
24	X-RAY DIFFRACTION	24	chain D and (resid 136:151 )
25	X-RAY DIFFRACTION	25	chain D and (resid 152:163 )
26	X-RAY DIFFRACTION	26	chain D and (resid 164:179 )
27	X-RAY DIFFRACTION	27	chain D and (resid 180:223 )
28	X-RAY DIFFRACTION	28	chain E and (resid 4:32 )
29	X-RAY DIFFRACTION	29	chain E and (resid 33:46 )
30	X-RAY DIFFRACTION	30	chain E and (resid 47:99 )
31	X-RAY DIFFRACTION	31	chain E and (resid 100:135 )
32	X-RAY DIFFRACTION	32	chain E and (resid 136:151 )
33	X-RAY DIFFRACTION	33	chain E and (resid 152:163 )
34	X-RAY DIFFRACTION	34	chain E and (resid 164:179 )
35	X-RAY DIFFRACTION	35	chain E and (resid 180:200 )
36	X-RAY DIFFRACTION	36	chain E and (resid 201:223 )
37	X-RAY DIFFRACTION	37	chain F and (resid 2:20 )
38	X-RAY DIFFRACTION	38	chain F and (resid 21:123 )
39	X-RAY DIFFRACTION	39	chain F and (resid 124:135 )
40	X-RAY DIFFRACTION	40	chain F and (resid 136:163 )
41	X-RAY DIFFRACTION	41	chain F and (resid 164:179 )
42	X-RAY DIFFRACTION	42	chain F and (resid 180:223 )
43	X-RAY DIFFRACTION	43	chain G and (resid 2:65 )
44	X-RAY DIFFRACTION	44	chain G and (resid 66:123 )
45	X-RAY DIFFRACTION	45	chain G and (resid 124:135 )
46	X-RAY DIFFRACTION	46	chain G and (resid 136:163 )
47	X-RAY DIFFRACTION	47	chain G and (resid 164:179 )
48	X-RAY DIFFRACTION	48	chain G and (resid 180:200 )
49	X-RAY DIFFRACTION	49	chain G and (resid 201:223 )
50	X-RAY DIFFRACTION	50	chain H and (resid 2:20 )
51	X-RAY DIFFRACTION	51	chain H and (resid 21:123 )
52	X-RAY DIFFRACTION	52	chain H and (resid 124:135 )
53	X-RAY DIFFRACTION	53	chain H and (resid 136:151 )
54	X-RAY DIFFRACTION	54	chain H and (resid 152:163 )
55	X-RAY DIFFRACTION	55	chain H and (resid 164:179 )
56	X-RAY DIFFRACTION	56	chain H and (resid 180:223 )