PDB-4ri8: FAN1 Nuclease bound to 5' phosphorylated p(dG)/3'(dT-dT-dT-dT) do...

ID or keywords:

Entry

Database: PDB / ID: 4ri8

Title

FAN1 Nuclease bound to 5' phosphorylated p(dG)/3'(dT-dT-dT-dT) double flap DNA

Components

DNA (5'-D(*AP*AP*CP*AP*CP*GP*CP*CP*TP*AP*GP*AP*CP*TP*CP*CP*TP*CP*A)-3')
DNA (5'-D(*TP*TP*TP*GP*AP*GP*GP*AP*GP*TP*CP*TP*TP*T)-3')
DNA (5'-D(P*GP*AP*GP*GP*CP*GP*TP*G)-3')
Fanconi-associated nuclease 1

Keywords

hydrolase/dna /

nuclease / hydrolase-dna complex

Function / homology

Function and homology information

flap-structured DNA binding /

phosphodiesterase I / 5'-flap endonuclease activity /

phosphodiesterase I activity / 5'-3' exonuclease activity /

Hydrolases; Acting on ester bonds; Endodeoxyribonucleases producing 5'-phosphomonoesters / ubiquitin-modified protein reader activity /

intercellular bridge / interstrand cross-link repair / nucleotide-excision repair ...
Similarity search - Function

Biological species

Homo sapiens (human)

Method

X-RAY DIFFRACTION /

SYNCHROTRON / Resolution: 2.9 Å

Authors

Pavletich, N.P. / Wang, R.

Citation

Journal: Science / Year: 2014
Title: DNA repair. Mechanism of DNA interstrand cross-link processing by repair nuclease FAN1.
Authors: Wang, R. / Persky, N.S. / Yoo, B. / Ouerfelli, O. / Smogorzewska, A. / Elledge, S.J. / Pavletich, N.P.

History

Deposition	Oct 5, 2014	Deposition site: RCSB / Processing site: RCSB
Revision 1.0	Dec 3, 2014	Provider: repository / Type: Initial release
Revision 1.1	Dec 10, 2014	Group: Database references
Revision 1.2	Feb 28, 2024	Group: Data collection / Database references / Derived calculations Category: chem_comp_atom / chem_comp_bond ...chem_comp_atom / chem_comp_bond / database_2 / pdbx_struct_conn_angle / struct_conn / struct_ref_seq_dif / struct_site Item: _database_2.pdbx_DOI / _database_2.pdbx_database_accession ..._database_2.pdbx_DOI / _database_2.pdbx_database_accession / _pdbx_struct_conn_angle.ptnr1_auth_asym_id / _pdbx_struct_conn_angle.ptnr1_auth_comp_id / _pdbx_struct_conn_angle.ptnr1_auth_seq_id / _pdbx_struct_conn_angle.ptnr1_label_asym_id / _pdbx_struct_conn_angle.ptnr1_label_atom_id / _pdbx_struct_conn_angle.ptnr1_label_comp_id / _pdbx_struct_conn_angle.ptnr1_label_seq_id / _pdbx_struct_conn_angle.ptnr2_auth_asym_id / _pdbx_struct_conn_angle.ptnr2_label_asym_id / _pdbx_struct_conn_angle.ptnr3_auth_asym_id / _pdbx_struct_conn_angle.ptnr3_auth_comp_id / _pdbx_struct_conn_angle.ptnr3_auth_seq_id / _pdbx_struct_conn_angle.ptnr3_label_asym_id / _pdbx_struct_conn_angle.ptnr3_label_atom_id / _pdbx_struct_conn_angle.ptnr3_label_comp_id / _pdbx_struct_conn_angle.ptnr3_label_seq_id / _pdbx_struct_conn_angle.value / _struct_conn.pdbx_dist_value / _struct_conn.ptnr1_auth_asym_id / _struct_conn.ptnr1_auth_comp_id / _struct_conn.ptnr1_auth_seq_id / _struct_conn.ptnr1_label_asym_id / _struct_conn.ptnr1_label_atom_id / _struct_conn.ptnr1_label_comp_id / _struct_conn.ptnr1_label_seq_id / _struct_conn.ptnr2_auth_asym_id / _struct_conn.ptnr2_auth_comp_id / _struct_conn.ptnr2_auth_seq_id / _struct_conn.ptnr2_label_asym_id / _struct_conn.ptnr2_label_atom_id / _struct_conn.ptnr2_label_comp_id / _struct_conn.ptnr2_label_seq_id / _struct_ref_seq_dif.details / _struct_site.pdbx_auth_asym_id / _struct_site.pdbx_auth_comp_id / _struct_site.pdbx_auth_seq_id

Structure viewer	Molecule: MolmilJmol/JSmol

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Download

PDBx/mmCIF format	4ri8.cif.gz	602 KB	Display	PDBx/mmCIF format
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Validation report

Arichive directory	https://data.pdbj.org/pub/pdb/validation_reports/ri/4ri8 ftp://data.pdbj.org/pub/pdb/validation_reports/ri/4ri8	HTTPS FTP

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Related structure data

Related structure data	4ri9C 4riaC 4ribC 4ricC 4ridC C: citing same article (ref.)
Similar structure data	4ri9-2 7khs-1 7k71-d 3hxm-d 7k6o-d 5un8-1 5un8-2 4nz8-d 4rsn-d 3f73-1 Search similar-shape structures by Omokage search (details) Search similar-shape structures by Omokage search (details) Search similar-shape structures by Omokage search (details) Similarity search - Function & homologyF&H Search Similarity search - FunctionF&H Search Similarity search - HomologyF&H Search

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Links

PDB pages	PDBj / wwPDB / NCBI
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PDB pages

PDBj /

wwPDB /

NCBI

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Deposited unit

A: Fanconi-associated nuclease 1

B: Fanconi-associated nuclease 1

E: DNA (5'-D(*TP*TP*TP*GP*AP*GP*GP*AP*GP*TP*CP*TP*TP*T)-3')

F: DNA (5'-D(P*GP*AP*GP*GP*CP*GP*TP*G)-3')

G: DNA (5'-D(*AP*AP*CP*AP*CP*GP*CP*CP*TP*AP*GP*AP*CP*TP*CP*CP*TP*CP*A)-3')

H: DNA (5'-D(*TP*TP*TP*GP*AP*GP*GP*AP*GP*TP*CP*TP*TP*T)-3')

I: DNA (5'-D(P*GP*AP*GP*GP*CP*GP*TP*G)-3')

J: DNA (5'-D(*AP*AP*CP*AP*CP*GP*CP*CP*TP*AP*GP*AP*CP*TP*CP*CP*TP*CP*A)-3')

hetero molecules

173 kDa, 8 polymers
Search similar-shape structures of this assembly by Omokage search (details)

1

A: Fanconi-associated nuclease 1

E: DNA (5'-D(*TP*TP*TP*GP*AP*GP*GP*AP*GP*TP*CP*TP*TP*T)-3')

F: DNA (5'-D(P*GP*AP*GP*GP*CP*GP*TP*G)-3')

G: DNA (5'-D(*AP*AP*CP*AP*CP*GP*CP*CP*TP*AP*GP*AP*CP*TP*CP*CP*TP*CP*A)-3')

hetero molecules

defined by author&software
86.5 kDa, 4 polymers
Search similar-shape structures of this assembly by Omokage search (details)

2

B: Fanconi-associated nuclease 1

H: DNA (5'-D(*TP*TP*TP*GP*AP*GP*GP*AP*GP*TP*CP*TP*TP*T)-3')

I: DNA (5'-D(P*GP*AP*GP*GP*CP*GP*TP*G)-3')

J: DNA (5'-D(*AP*AP*CP*AP*CP*GP*CP*CP*TP*AP*GP*AP*CP*TP*CP*CP*TP*CP*A)-3')

hetero molecules

defined by author&software
86.5 kDa, 4 polymers
Search similar-shape structures of this assembly by Omokage search (details)

Unit cell

Length a, b, c (Å)	62.260, 210.880, 83.220
Angle α, β, γ (deg.)	90.00, 107.61, 90.00
Int Tables number	4
Space group name H-M	P12₁1

Noncrystallographic symmetry (NCS)

NCS domain:
NCS domain segments:

Dom-ID	Component-ID	Ens-ID	Refine code	Auth asym-ID	Auth seq-ID
1	1	1	1	A	370 - 426
2	1	1	1	B	370 - 426
1	1	2	1	A	538 - 552
2	1	2	1	B	538 - 552
1	1	3	1	A	427 - 459
2	1	3	1	B	427 - 459
1	1	4	1	A	533 - 537
2	1	4	1	B	533 - 537
1	1	5	1	A	460 - 532
2	1	5	1	B	460 - 532
1	1	6	1	A	593 - 773
2	1	6	1	B	593 - 773
1	1	7	1	A	774 - 787
2	1	7	1	B	774 - 787
1	1	8	1	A	791 - 799
2	1	8	1	B	791 - 799
1	1	9	1	A	810 - 834
2	1	9	1	B	810 - 834
1	1	10	1	A	956 - 1009
2	1	10	1	B	956 - 1009
1	1	11	1	A	572 - 592
2	1	11	1	B	572 - 592
1	1	12	1	A	835 - 955
2	1	12	1	B	835 - 955
1	1	13	1	E	1 - 14
2	1	13	1	H	1 - 14
1	1	14	1	G	10 - 19
2	1	14	1	J	10 - 19
1	1	15	1	F	0 - 7
2	1	15	1	I	0 - 7
1	1	16	1	G	1 - 9
2	1	16	1	J	1 - 9

NCS ensembles :

#1: Protein	Fanconi-associated nuclease 1 / FANCD2/FANCI-associated nuclease 1 / Myotubularin-related protein 15 Mass: 73926.023 Da / Num. of mol.: 2 / Fragment: unp residues 370-1017 / Mutation: V487A Source method: isolated from a genetically manipulated source Source: (gene. exp.) Homo sapiens (human) / Gene: FAN1, KIAA1018, MTMR15 / Plasmid: pET15b / Production host: Escherichia coli (E. coli) / Strain (production host): BL21(DE3) References: UniProt: Q9Y2M0, Hydrolases; Acting on ester bonds; Endodeoxyribonucleases producing 5'-phosphomonoesters, phosphodiesterase I
#2: DNA chain	DNA (5'-D(TPTPTPGPAPGPGPAPGPTPCPTPTPT)-3') Mass: 4316.811 Da / Num. of mol.: 2 / Source method: obtained synthetically
#3: DNA chain	DNA (5'-D(PGPAPGPGPCPGPTPG)-3') Mass: 2507.653 Da / Num. of mol.: 2 / Source method: obtained synthetically
#4: DNA chain	DNA (5'-D(APAPCPAPCPGPCPCPTPAPGPAPCPTPCPCPTPCPA)-3') Mass: 5718.727 Da / Num. of mol.: 2 / Source method*: obtained synthetically
#5: Chemical	ChemComp-CA / CALCIUM ION Mass: 40.078 Da / Num. of mol.: 2 / Source method: obtained synthetically / Formula: Ca

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Experiment

Experiment	Method: X-RAY DIFFRACTION / Number of used crystals: 1

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Sample preparation

Crystal	Density Matthews: 3.14 Å³/Da / Density % sol: 60.83 %
Crystal grow	Temperature: 277 K / Method: vapor diffusion, hanging drop / pH: 7 Details: PEG 3350, NaCl, pH 7.0, hanging drop vapor diffusion, temperature 277K, VAPOR DIFFUSION, HANGING DROP

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Data collection

Diffraction

Mean temperature: 100 K

Diffraction source

Source:

SYNCHROTRON / Site:

APS

/ Beamline: 24-ID-C / Wavelength: 0.97925 Å

Detector

Type: ADSC QUANTUM 315 / Detector: CCD / Date: Feb 2, 2013

Radiation

Monochromator: mirror / Protocol: SINGLE WAVELENGTH / Monochromatic (M) / Laue (L): M / Scattering type: x-ray

Radiation wavelength

Wavelength: 0.97925 Å / Relative weight: 1

Reflection

Resolution: 2.9→80 Å / Num. obs: 42278 / % possible obs: 93.7 % / Observed criterion σ(F): 0 / Observed criterion σ(I): 0 / Redundancy: 7.4 % / R_merge(I) obs: 0.098 / Net I/σ(I): 6.8

Reflection shell

Resolution (Å)	Redundancy (%)	R_merge(I) obs	Diffraction-ID	% possible all
2.9-3	7.2	0.833	1	94.7
3-3.12	6.9	0.594	1	92.2
3.12-3.27	7.6	0.332	1	96.1
3.27-3.44	7.5	0.239	1	96
3.44-3.65	7.4	0.188	1	93.9
3.65-3.94	7.2	0.14	1	92
3.94-4.33	7.6	0.098	1	95.4
4.33-4.96	7.4	0.082	1	92.7
4.96-6.25	7.6	0.08	1	94.3
6.25-80	7.5	0.046	1	90.2

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Processing

Software

Name	Version	Classification
SCALEPACK		data scaling
REFMAC	5.7.0032	refinement
PDB_EXTRACT	3.15	data extraction
ADSC	Quantum	data collection
DENZO		data reduction
SHARP		phasing

Refinement

Resolution: 2.9→70 Å / Cor.coef. F_o:F_c: 0.944 / Cor.coef. F_o:F_c free: 0.926 / SU B: 44.259 / SU ML: 0.361 / Cross valid method: THROUGHOUT / ESU R Free: 0.423 / Stereochemistry target values: MAXIMUM LIKELIHOOD

	R_factor	Num. reflection	% reflection	Selection details
R_free	0.25476	1647	4.1 %	RANDOM
R_work	0.22053	-	-	-
obs	0.22193	38976	90.08 %	-

Solvent computation

Ion probe radii: 1.1 Å / Shrinkage radii: 1.1 Å / VDW probe radii: 1.3 Å / Solvent model: MASK

Displacement parameters

B_iso mean: 97.73 Å²

	B_aniso -1	B_aniso -2	B_aniso -3
1-	0.73 Å²	0 Å²	-2.19 Å²
2-	-	-4.54 Å²	0 Å²
3-	-	-	3.74 Å²

Refinement step

Cycle: LAST / Resolution: 2.9→70 Å

	Protein	Nucleic acid	Ligand	Solvent	Total
Num. atoms	9884	1672	2	0	11558

Refine LS restraints

Refine-ID	Type	Dev ideal	Dev ideal target	Number
X-RAY DIFFRACTION	r_bond_refined_d	0.009	0.018	11970
X-RAY DIFFRACTION	r_bond_other_d
X-RAY DIFFRACTION	r_angle_refined_deg	1.315	1.822	16529
X-RAY DIFFRACTION	r_angle_other_deg
X-RAY DIFFRACTION	r_dihedral_angle_1_deg	5.786	5	1227
X-RAY DIFFRACTION	r_dihedral_angle_2_deg	33.769	23.151	476
X-RAY DIFFRACTION	r_dihedral_angle_3_deg	19.793	15	1820
X-RAY DIFFRACTION	r_dihedral_angle_4_deg	15.913	15	90
X-RAY DIFFRACTION	r_chiral_restr	0.088	0.2	1778
X-RAY DIFFRACTION	r_gen_planes_refined	0.005	0.021	8418
X-RAY DIFFRACTION	r_gen_planes_other
X-RAY DIFFRACTION	r_nbd_refined
X-RAY DIFFRACTION	r_nbd_other
X-RAY DIFFRACTION	r_nbtor_refined
X-RAY DIFFRACTION	r_nbtor_other
X-RAY DIFFRACTION	r_xyhbond_nbd_refined
X-RAY DIFFRACTION	r_xyhbond_nbd_other
X-RAY DIFFRACTION	r_metal_ion_refined
X-RAY DIFFRACTION	r_metal_ion_other
X-RAY DIFFRACTION	r_symmetry_vdw_refined
X-RAY DIFFRACTION	r_symmetry_vdw_other
X-RAY DIFFRACTION	r_symmetry_hbond_refined
X-RAY DIFFRACTION	r_symmetry_hbond_other
X-RAY DIFFRACTION	r_symmetry_metal_ion_refined
X-RAY DIFFRACTION	r_symmetry_metal_ion_other
X-RAY DIFFRACTION	r_mcbond_it	1.288	1.538	4916
X-RAY DIFFRACTION	r_mcbond_other
X-RAY DIFFRACTION	r_mcangle_it	2.252	3.457	6132
X-RAY DIFFRACTION	r_mcangle_other
X-RAY DIFFRACTION	r_scbond_it	1.359	1.498	7054
X-RAY DIFFRACTION	r_scbond_other
X-RAY DIFFRACTION	r_scangle_it
X-RAY DIFFRACTION	r_scangle_other
X-RAY DIFFRACTION	r_long_range_B_refined
X-RAY DIFFRACTION	r_long_range_B_other
X-RAY DIFFRACTION	r_rigid_bond_restr
X-RAY DIFFRACTION	r_sphericity_free
X-RAY DIFFRACTION	r_sphericity_bonded

Refine LS restraints NCS

Dom-ID: 1 / Refine-ID: X-RAY DIFFRACTION

Ens-ID	Auth asym-ID	Number	Type	Rms dev position (Å)	Weight position
1	A	426	TIGHT POSITIONAL	0.04	0.05
1	A	170	TIGHT POSITIONAL	0.05	0.05
1	A	957	TIGHT POSITIONAL	0.06	0.05
1	A	286	TIGHT POSITIONAL	0.06	0.05
1	A	201	TIGHT POSITIONAL	0.04	0.05
1	A	171	TIGHT POSITIONAL	0.04	0.05
1	A	178	TIGHT POSITIONAL	0.05	0.05
1	A	479	TIGHT THERMAL	2.3	99
2	A	120	TIGHT THERMAL	1.52	99
3	A	268	TIGHT THERMAL	2.57	99
4	A	41	TIGHT THERMAL	2.52	99
5	A	489	TIGHT THERMAL	3.34	99
6	A	1512	TIGHT THERMAL	3.12	99
7	A	108	TIGHT THERMAL	3.05	99
8	A	48	TIGHT THERMAL	4.51	99
9	A	195	TIGHT THERMAL	5.36	99
10	A	426	TIGHT THERMAL	2.81	99
11	A	170	TIGHT THERMAL	1.47	99
12	A	957	TIGHT THERMAL	3.21	99
13	E	286	TIGHT THERMAL	4.83	99
14	G	201	TIGHT THERMAL	2.29	99
15	F	171	TIGHT THERMAL	11.27	99
16	G	178	TIGHT THERMAL	6.81	99

LS refinement shell

Resolution: 2.902→2.977 Å / Total num. of bins used: 20

	R_factor	Num. reflection	% reflection
R_free	0.413	119	-
R_work	0.361	2709	-
obs	-	-	84.22 %

Refinement TLS params.

Method: refined / Refine-ID: X-RAY DIFFRACTION

ID	L11 (°²)	L12 (°²)	L13 (°²)	L22 (°²)	L23 (°²)	L33 (°²)	S11 (Å °)	S12 (Å °)	S13 (Å °)	S21 (Å °)	S22 (Å °)	S23 (Å °)	S31 (Å °)	S32 (Å °)	S33 (Å °)	T11 (Å²)	T12 (Å²)	T13 (Å²)	T22 (Å²)	T23 (Å²)	T33 (Å²)	Origin x (Å)	Origin y (Å)	Origin z (Å)
1	5.3662	1.9689	-4.3695	4.3662	-4.0704	5.5006	0.1928	-0.3045	-0.0136	-0.3761	-0.6667	-0.7498	-0.0901	1.018	0.4738	1.1105	-0.0452	-0.1343	0.7447	-0.0817	1.1797	62.245	-3.951	91.477
2	7.1365	1.0743	-1.0471	5.3372	-2.151	3.1245	0.346	0.4789	0.504	-0.2842	-0.401	-0.6133	0.1974	0.7164	0.055	1.2815	0.0346	-0.1295	0.6951	-0.1323	0.8524	45.019	-86.442	33.65
3	3.4606	-2.7184	0.2739	16.2168	-3.3869	4.1681	-0.0553	0.1558	0.2168	-0.1602	0.146	-0.2817	-0.3462	0.3025	-0.0908	1.0999	-0.0468	-0.1472	0.6056	0.1885	0.7645	63.964	-9.545	61.78
4	3.6008	-3.4783	0.6685	14.6807	-3.4601	3.095	0.1067	0.2708	-0.2391	-0.7338	-0.2349	0.24	0.2138	0.0282	0.1281	0.8847	0.2697	-0.0871	0.5892	0.1305	0.7773	67.384	-79.69	52.403
5	11.3376	4.0103	0.2982	5.1202	-0.0198	7.0455	0.0467	-0.816	0.6194	0.0491	-0.5637	0.6616	-0.3353	-0.0571	0.517	0.4924	0.0748	-0.012	0.4289	-0.0422	0.4963	54.131	-35.45	71.745
6	0.9093	-0.9807	1.0511	1.2374	-1.3716	1.5703	0.3227	0.237	-0.0021	-0.0624	0.0067	0.3792	-0.2156	-0.0466	-0.3294	1.7532	0.2561	-0.3278	1.3813	0.2102	1.8261	41.533	-8.734	50.165
7	3.7505	-1.9959	2.2241	8.3221	0.9865	7.6095	-0.3289	0.8912	0.7543	-1.4058	-0.085	0.2749	-0.4733	0.272	0.4139	0.9482	-0.0804	-0.2571	0.5944	0.0375	0.6378	54.633	-28.707	55.223
8	2.8774	0.8232	0.8672	6.2807	2.4419	4.9685	-0.1949	-0.4829	0.3958	0.4113	-0.4255	0.693	-0.3087	-0.8047	0.6204	0.1498	0.0876	0.1623	0.6036	-0.2632	0.7651	46.038	-16.548	96.477
9	6.2682	0.3047	-0.9832	8.0683	0.2699	3.8641	0.0621	-0.0987	0.8803	0.4576	0.0182	-1.0174	-0.6155	0.409	-0.0804	0.2939	-0.0133	-0.089	0.6707	-0.1982	0.7925	79.601	-12.011	98.283
10	4.2123	-0.7761	1.1238	3.5373	0.5769	4.1633	-0.2074	-0.298	0.0708	0.6411	0.0731	-0.2669	0.369	0.263	0.1344	0.2907	0.0844	0.1152	0.331	-0.0207	0.2922	70.787	-35.215	95.705
11	10.6946	-1.283	-2.7522	4.0522	-1.2318	8.1163	-0.206	0.4772	-0.1318	-0.1606	-0.0694	0.4336	0.4977	-0.4961	0.2755	0.6481	0.0624	-0.1151	0.3804	-0.0379	0.4836	49.172	-56.707	56.871
12	3.2648	0.0285	1.349	6.387	-0.8575	1.142	0.0116	-0.5418	-1.1407	0.8258	0.0321	-0.0957	0.395	0.0004	-0.0437	1.375	0.1953	0.0311	1.0257	0.2629	1.0244	62.684	-84.152	76.699
13	5.918	-0.3384	2.0571	10.3764	-2.1904	4.6378	-0.3911	-0.8901	0.1823	0.4507	0.1983	-0.8537	0.335	0.6103	0.1927	0.7624	0.1521	-0.0793	0.6713	-0.0655	0.4045	63.534	-62.219	65.882
14	4.2751	0.228	1.1095	4.4722	1.6402	2.746	0.1114	-0.2253	-0.3559	0.0427	0.0364	0.458	0.7312	-0.5731	-0.1477	0.7878	-0.2094	0.0153	0.5442	0.1693	0.4651	28.916	-78.701	44.787
15	7.2564	0.6955	-2.1455	5.4932	-1.597	4.0304	0.0674	0.6014	-0.8112	-0.5784	-0.1515	-0.49	1.022	0.5288	0.0841	1.1384	0.0455	0.0451	0.947	-0.108	0.4883	47.92	-74.724	17.348
16	2.3084	-0.6816	0.2922	3.3196	-0.8558	4.6526	-0.0881	0.2951	0.4808	-0.0331	-0.0545	-0.2514	0.2306	0.0468	0.1427	0.422	-0.0393	-0.0484	0.3835	0.1066	0.4582	40.055	-54.323	29.531

Refinement TLS group

ID	Refine-ID	Refine TLS-ID	Auth asym-ID	Auth seq-ID
1	X-RAY DIFFRACTION	1	F	0 - 7
2	X-RAY DIFFRACTION	1	G	1 - 9
3	X-RAY DIFFRACTION	2	I	0 - 7
4	X-RAY DIFFRACTION	2	J	1 - 9
5	X-RAY DIFFRACTION	3	E	1 - 14
6	X-RAY DIFFRACTION	3	G	10 - 19
7	X-RAY DIFFRACTION	4	H	1 - 14
8	X-RAY DIFFRACTION	4	J	10 - 19
9	X-RAY DIFFRACTION	5	A	370 - 410
10	X-RAY DIFFRACTION	5	A	540 - 582
11	X-RAY DIFFRACTION	6	A	457 - 532
12	X-RAY DIFFRACTION	7	A	411 - 456
13	X-RAY DIFFRACTION	7	A	533 - 539
14	X-RAY DIFFRACTION	8	A	592 - 773
15	X-RAY DIFFRACTION	9	A	774 - 835
16	X-RAY DIFFRACTION	9	A	957 - 1009
17	X-RAY DIFFRACTION	10	A	836 - 956
18	X-RAY DIFFRACTION	10	A	583 - 591
19	X-RAY DIFFRACTION	11	B	370 - 410
20	X-RAY DIFFRACTION	11	B	540 - 582
21	X-RAY DIFFRACTION	12	B	457 - 532
22	X-RAY DIFFRACTION	13	B	411 - 456
23	X-RAY DIFFRACTION	13	B	533 - 539
24	X-RAY DIFFRACTION	14	B	592 - 773
25	X-RAY DIFFRACTION	15	B	774 - 835
26	X-RAY DIFFRACTION	15	B	957 - 1009
27	X-RAY DIFFRACTION	16	B	836 - 956
28	X-RAY DIFFRACTION	16	B	583 - 591

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