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- PDB-4r3l: Crystal structure of Ard1 N-terminal acetyltransferase from Sulfo... -
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Open data
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Basic information
Entry | Database: PDB / ID: 4r3l | ||||||
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Title | Crystal structure of Ard1 N-terminal acetyltransferase from Sulfolobus solfataricus bound to substrate peptide fragment and CoA | ||||||
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![]() | TRANSFERASE/TRANSFERASE SUBSTRATE / Protein-substrate complex / ![]() ![]() | ||||||
Function / homology | ![]() N-terminal amino-acid Nalpha-acetyltransferase NatA / peptide-glutamate-alpha-N-acetyltransferase activity / N-terminal methionine Nalpha-acetyltransferase NatE / NatA complex / peptide-serine-alpha-N-acetyltransferase activity / ![]() ![]() ![]() ![]() ![]() ![]() ![]() ![]() ![]() ![]() ![]() Similarity search - Function | ||||||
Biological species | ![]() ![]() ![]() ![]() ![]() | ||||||
Method | ![]() ![]() ![]() | ||||||
![]() | Chang, Y.Y. / Hsu, C.H. | ||||||
![]() | ![]() Title: Structural Basis for Substrate-specific Acetylation of N alpha-acetyltransferase Ard1 from Sulfolobus solfataricus Authors: Chang, Y.Y. / Hsu, C.H. | ||||||
History |
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Structure visualization
Structure viewer | Molecule: ![]() ![]() |
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Downloads & links
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Download
PDBx/mmCIF format | ![]() | 50 KB | Display | ![]() |
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PDB format | ![]() | 34.6 KB | Display | ![]() |
PDBx/mmJSON format | ![]() | Tree view | ![]() | |
Others | ![]() |
-Validation report
Arichive directory | ![]() ![]() | HTTPS FTP |
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-Related structure data
Related structure data | ![]() 4r3kC ![]() 2x7bS C: citing same article ( S: Starting model for refinement |
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Similar structure data |
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Links
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Assembly
Deposited unit | ![]()
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Unit cell |
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Components
#1: Protein | Mass: 20302.441 Da / Num. of mol.: 1 Source method: isolated from a genetically manipulated source Source: (gene. exp.) ![]() ![]() ![]() ![]() ![]() References: UniProt: Q980R9, ![]() |
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#2: Protein/peptide | Mass: 548.545 Da / Num. of mol.: 1 / Source method: obtained synthetically Details: the peptide sequence derived from Alba protein in Sulfolobus solfataricus Source: (synth.) ![]() ![]() ![]() |
#3: Chemical | ChemComp-COA / ![]() |
#4: Water | ChemComp-HOH / ![]() |
-Experimental details
-Experiment
Experiment | Method: ![]() |
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Sample preparation
Crystal | Density Matthews: 2.06 Å3/Da / Density % sol: 40.24 % |
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Crystal grow![]() | Temperature: 283 K / Method: vapor diffusion, sitting drop / pH: 4.2 Details: 12.5% PEG 4000, 0.1M sodium acetate trihydrate pH 4.2, 0.1M ammonium sulfate, VAPOR DIFFUSION, SITTING DROP, temperature 283K |
-Data collection
Diffraction | Mean temperature: 100 K |
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Diffraction source | Source: ![]() ![]() ![]() |
Detector | Type: RAYONIX MX300HE / Detector: CCD / Date: Jul 28, 2013 |
Radiation | Monochromator: Si 111 CHANNEL / Protocol: SINGLE WAVELENGTH / Monochromatic (M) / Laue (L): M / Scattering type: x-ray |
Radiation wavelength | Wavelength![]() |
Reflection | Resolution: 1.839→30 Å / Num. obs: 15532 / % possible obs: 99.8 % / Observed criterion σ(F): 0 / Observed criterion σ(I): -3 / Redundancy: 4.7 % / Rmerge(I) obs: 0.044 / Rsym value: 0.044 |
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Processing
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Refinement | Method to determine structure![]() ![]() Starting model: 2X7B Resolution: 1.839→23.875 Å / σ(F): 0 / σ(I): -3 / Stereochemistry target values: ML
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Refinement step | Cycle: LAST / Resolution: 1.839→23.875 Å
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Refine LS restraints |
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LS refinement shell | Refine-ID: X-RAY DIFFRACTION / Total num. of bins used: 11
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