+データを開く
-基本情報
登録情報 | データベース: PDB / ID: 4q21 | ||||||
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タイトル | MOLECULAR SWITCH FOR SIGNAL TRANSDUCTION: STRUCTURAL DIFFERENCES BETWEEN ACTIVE AND INACTIVE FORMS OF PROTOONCOGENIC RAS PROTEINS | ||||||
要素 | C-H-RAS P21 PROTEIN CATALYTIC DOMAIN | ||||||
キーワード | ONCOGENE PROTEIN (がん遺伝子) | ||||||
機能・相同性 | 機能・相同性情報 GTPase complex / oncogene-induced cell senescence / positive regulation of ruffle assembly / negative regulation of GTPase activity / regulation of neurotransmitter receptor localization to postsynaptic specialization membrane / T-helper 1 type immune response / positive regulation of wound healing / positive regulation of miRNA metabolic process / defense response to protozoan / Signaling by RAS GAP mutants ...GTPase complex / oncogene-induced cell senescence / positive regulation of ruffle assembly / negative regulation of GTPase activity / regulation of neurotransmitter receptor localization to postsynaptic specialization membrane / T-helper 1 type immune response / positive regulation of wound healing / positive regulation of miRNA metabolic process / defense response to protozoan / Signaling by RAS GAP mutants / Signaling by RAS GTPase mutants / Activation of RAS in B cells / RAS signaling downstream of NF1 loss-of-function variants / SOS-mediated signalling / Activated NTRK3 signals through RAS / Activated NTRK2 signals through RAS / SHC1 events in ERBB4 signaling / Signalling to RAS / positive regulation of protein targeting to membrane / SHC-related events triggered by IGF1R / Activated NTRK2 signals through FRS2 and FRS3 / Estrogen-stimulated signaling through PRKCZ / adipose tissue development / SHC-mediated cascade:FGFR3 / MET activates RAS signaling / : / Schwann cell development / PTK6 Regulates RHO GTPases, RAS GTPase and MAP kinases / Signaling by PDGFRA transmembrane, juxtamembrane and kinase domain mutants / Signaling by PDGFRA extracellular domain mutants / SHC-mediated cascade:FGFR2 / SHC-mediated cascade:FGFR4 / Signaling by FGFR4 in disease / SHC-mediated cascade:FGFR1 / Erythropoietin activates RAS / protein-membrane adaptor activity / FRS-mediated FGFR3 signaling / Signaling by FLT3 ITD and TKD mutants / FRS-mediated FGFR2 signaling / FRS-mediated FGFR4 signaling / Signaling by FGFR3 in disease / FRS-mediated FGFR1 signaling / p38MAPK events / Tie2 Signaling / Signaling by FGFR2 in disease / GRB2 events in EGFR signaling / EPHB-mediated forward signaling / SHC1 events in EGFR signaling / EGFR Transactivation by Gastrin / Signaling by FLT3 fusion proteins / FLT3 Signaling / 髄鞘 / Signaling by FGFR1 in disease / Ras activation upon Ca2+ influx through NMDA receptor / GRB2 events in ERBB2 signaling / NCAM signaling for neurite out-growth / CD209 (DC-SIGN) signaling / SHC1 events in ERBB2 signaling / Downstream signal transduction / Constitutive Signaling by Overexpressed ERBB2 / Insulin receptor signalling cascade / intrinsic apoptotic signaling pathway / 低分子量GTPアーゼ / G protein activity / Signaling by phosphorylated juxtamembrane, extracellular and kinase domain KIT mutants / VEGFR2 mediated cell proliferation / positive regulation of epithelial cell proliferation / regulation of actin cytoskeleton organization / FCERI mediated MAPK activation / animal organ morphogenesis / positive regulation of JNK cascade / Signaling by ERBB2 TMD/JMD mutants / regulation of long-term neuronal synaptic plasticity / RAF activation / positive regulation of MAP kinase activity / Signaling by high-kinase activity BRAF mutants / Constitutive Signaling by EGFRvIII / MAP2K and MAPK activation / Signaling by ERBB2 ECD mutants / Signaling by ERBB2 KD Mutants / Signaling by SCF-KIT / cellular response to gamma radiation / positive regulation of GTPase activity / エンドサイトーシス / Regulation of RAS by GAPs / Negative regulation of MAPK pathway / RAS processing / Signaling by RAF1 mutants / GDP binding / Signaling by moderate kinase activity BRAF mutants / Paradoxical activation of RAF signaling by kinase inactive BRAF / Signaling downstream of RAS mutants / 走化性 / 分裂促進因子活性化タンパク質キナーゼ / positive regulation of type II interferon production / 細胞老化 / positive regulation of fibroblast proliferation / Signaling by BRAF and RAF1 fusions / DAP12 signaling / Constitutive Signaling by Ligand-Responsive EGFR Cancer Variants 類似検索 - 分子機能 | ||||||
生物種 | Homo sapiens (ヒト) | ||||||
手法 | X線回折 / 解像度: 2 Å | ||||||
データ登録者 | Kim, S.-H. | ||||||
引用 | ジャーナル: Science / 年: 1990 タイトル: Molecular switch for signal transduction: structural differences between active and inactive forms of protooncogenic ras proteins. 著者: Milburn, M.V. / Tong, L. / deVos, A.M. / Brunger, A. / Yamaizumi, Z. / Nishimura, S. / Kim, S.H. #1: ジャーナル: Proc.Natl.Acad.Sci.USA / 年: 1992 タイトル: X-Ray Crystal Structures of Transforming P21 Ras Mutants Suggest a Transition-State Stabilization Mechanism for GTP Hydrolysis 著者: Prive, G.G. / Milburn, M.V. / Tong, L. / Devos, A.M. / Yamaizumi, Z. / Nishimura, S. / Kim, S.-H. #2: ジャーナル: Proc.Natl.Acad.Sci.USA / 年: 1990 タイトル: Crystal Structure of an Active Form of Ras Protein, a Complex of a GTP Analog and the Hras P21 Catalytic Domain 著者: Brunger, A.T. / Milburn, M.V. / Tong, L. / Devos, A.M. / Jancarik, J. / Yamaizumi, Z. / Nishimura, S. / Ohtsuka, E. / Kim, S.-H. #3: ジャーナル: Science / 年: 1989 タイトル: Structure of Ras Protein 著者: Tong, L. / Milburn, M.V. / Devos, A.M. / Kim, S.-H. #4: ジャーナル: Science / 年: 1988 タイトル: Three-Dimensional Structure of an Oncogene Protein. Catalytic Domain of Human C-H-Ras P21 著者: Devos, A.M. / Tong, L. / Milburn, M.V. / Matias, P.M. / Jancarik, J. / Noguchi, S. / Nishimura, S. / Miura, K. / Ohtsuka, E. / Kim, S.-H. | ||||||
履歴 |
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-構造の表示
構造ビューア | 分子: MolmilJmol/JSmol |
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-ダウンロードとリンク
-ダウンロード
PDBx/mmCIF形式 | 4q21.cif.gz | 49.2 KB | 表示 | PDBx/mmCIF形式 |
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PDB形式 | pdb4q21.ent.gz | 35.3 KB | 表示 | PDB形式 |
PDBx/mmJSON形式 | 4q21.json.gz | ツリー表示 | PDBx/mmJSON形式 | |
その他 | その他のダウンロード |
-検証レポート
アーカイブディレクトリ | https://data.pdbj.org/pub/pdb/validation_reports/q2/4q21 ftp://data.pdbj.org/pub/pdb/validation_reports/q2/4q21 | HTTPS FTP |
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-関連構造データ
-リンク
-集合体
登録構造単位 |
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1 |
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単位格子 |
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Atom site foot note | 1: RESIDUES 60 - 64 ARE DISORDERED. |
-要素
#1: タンパク質 | 分子量: 21324.146 Da / 分子数: 1 / 由来タイプ: 組換発現 / 由来: (組換発現) Homo sapiens (ヒト) / 参照: UniProt: P01112 |
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#2: 化合物 | ChemComp-MG / |
#3: 化合物 | ChemComp-GDP / |
#4: 水 | ChemComp-HOH / |
-実験情報
-実験
実験 | 手法: X線回折 |
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-試料調製
結晶 | マシュー密度: 2.25 Å3/Da / 溶媒含有率: 45.29 % |
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-解析
ソフトウェア | 名称: X-PLOR / 分類: 精密化 | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
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精密化 | 解像度: 2→6 Å / Rfactor Rwork: 0.195 | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
精密化ステップ | サイクル: LAST / 解像度: 2→6 Å
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拘束条件 |
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