+Open data
-Basic information
Entry | Database: PDB / ID: 4mqx | ||||||
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Title | CLC-ec1 Fab Complex Cysless A399C-A432C mutant | ||||||
Components |
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Keywords | metal transport / membrane protein / alpha helical transmembrane protein / Cl/H antiporter | ||||||
Function / homology | Function and homology information chloride:proton antiporter activity / cellular stress response to acidic pH / voltage-gated chloride channel activity / immunoglobulin complex / immunoglobulin mediated immune response / chloride transmembrane transport / proton transmembrane transport / antigen binding / blood microparticle / identical protein binding / plasma membrane Similarity search - Function | ||||||
Biological species | Escherichia coli (E. coli) Mus musculus (house mouse) | ||||||
Method | X-RAY DIFFRACTION / SYNCHROTRON / MOLECULAR REPLACEMENT / Resolution: 3.516 Å | ||||||
Authors | Basilio, D. / Noack, K. / Picollo, A. / Accardi, A. | ||||||
Citation | Journal: Nat.Struct.Mol.Biol. / Year: 2014 Title: Conformational changes required for H(+)/Cl(-) exchange mediated by a CLC transporter. Authors: Basilio, D. / Noack, K. / Picollo, A. / Accardi, A. | ||||||
History |
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-Structure visualization
Structure viewer | Molecule: MolmilJmol/JSmol |
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-Downloads & links
-Download
PDBx/mmCIF format | 4mqx.cif.gz | 326.9 KB | Display | PDBx/mmCIF format |
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PDB format | pdb4mqx.ent.gz | 274.3 KB | Display | PDB format |
PDBx/mmJSON format | 4mqx.json.gz | Tree view | PDBx/mmJSON format | |
Others | Other downloads |
-Validation report
Arichive directory | https://data.pdbj.org/pub/pdb/validation_reports/mq/4mqx ftp://data.pdbj.org/pub/pdb/validation_reports/mq/4mqx | HTTPS FTP |
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-Related structure data
Related structure data | |
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Similar structure data |
-Links
-Assembly
Deposited unit |
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3 |
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Unit cell |
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-Components
#1: Protein | Mass: 49642.629 Da / Num. of mol.: 2 / Mutation: A399C 432C Source method: isolated from a genetically manipulated source Source: (gene. exp.) Escherichia coli (E. coli) / Strain: K12 / Gene: clcA, eriC, yadQ, b0155, JW5012 / Production host: Escherichia coli (E. coli) / References: UniProt: P37019 #2: Antibody | Mass: 23823.031 Da / Num. of mol.: 2 / Mutation: A399C 432C Source method: isolated from a genetically manipulated source Source: (gene. exp.) Mus musculus (house mouse) / Cell (production host): Hybridoma / Production host: mus musculus (house mouse) / References: UniProt: P01808*PLUS #3: Antibody | Mass: 23088.443 Da / Num. of mol.: 2 Source method: isolated from a genetically manipulated source Source: (gene. exp.) Mus musculus (house mouse) / Cell (production host): Hybridoma / Production host: mus musculus (house mouse) #4: Chemical | ChemComp-CL / |
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-Experimental details
-Experiment
Experiment | Method: X-RAY DIFFRACTION / Number of used crystals: 1 |
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-Sample preparation
Crystal | Density Matthews: 3.64 Å3/Da / Density % sol: 66.18 % |
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Crystal grow | Temperature: 293 K / Method: vapor diffusion, hanging drop / pH: 7.5 Details: 26% PEG 400, 0.1M sodium chloride, 0.05M HEPES, pH 7.5, VAPOR DIFFUSION, HANGING DROP, temperature 293K |
-Data collection
Diffraction | Mean temperature: 200 K |
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Diffraction source | Source: SYNCHROTRON / Site: NSLS / Beamline: X25 / Wavelength: 1.5 Å |
Detector | Type: PSI PILATUS 6M / Detector: PIXEL / Date: Nov 11, 2012 |
Radiation | Monochromator: monochromator/sagital focusing / Protocol: SINGLE WAVELENGTH / Monochromatic (M) / Laue (L): M / Scattering type: x-ray |
Radiation wavelength | Wavelength: 1.5 Å / Relative weight: 1 |
Reflection | Resolution: 3.5→50 Å / Num. obs: 34345 |
-Processing
Software |
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Refinement | Method to determine structure: MOLECULAR REPLACEMENT / Resolution: 3.516→43.556 Å / SU ML: 0.3 / σ(F): 1.34 / Phase error: 28.79 / Stereochemistry target values: ML
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Solvent computation | Shrinkage radii: 0.9 Å / VDW probe radii: 1.11 Å / Solvent model: FLAT BULK SOLVENT MODEL | |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Refinement step | Cycle: LAST / Resolution: 3.516→43.556 Å
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Refine LS restraints |
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LS refinement shell |
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