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- PDB-4kx6: Plasticity of the quinone-binding site of the complex II homolog ... -
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Open data
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Basic information
Entry | Database: PDB / ID: 4kx6 | ||||||
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Title | Plasticity of the quinone-binding site of the complex II homolog quinol:fumarate reductase | ||||||
![]() | (Fumarate reductase ...![]() | ||||||
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Function / homology | ![]() plasma membrane fumarate reductase complex / ![]() ![]() ![]() ![]() ![]() ![]() ![]() ![]() ![]() ![]() ![]() ![]() ![]() Similarity search - Function | ||||||
Biological species | ![]() ![]() ![]() | ||||||
Method | ![]() ![]() ![]() | ||||||
![]() | Singh, P.K. / Sarwar, M. / Maklashina, E. / Kotlyar, V. / Rajagukguk, S. / Tomasiak, T.M. / Cecchini, G. / Iverson, T.M. | ||||||
![]() | ![]() Title: Plasticity of the Quinone-binding Site of the Complex II Homolog Quinol:Fumarate Reductase. Authors: Singh, P.K. / Sarwar, M. / Maklashina, E. / Kotlyar, V. / Rajagukguk, S. / Tomasiak, T.M. / Cecchini, G. / Iverson, T.M. | ||||||
History |
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Structure visualization
Structure viewer | Molecule: ![]() ![]() |
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Downloads & links
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Download
PDBx/mmCIF format | ![]() | 831.8 KB | Display | ![]() |
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PDB format | ![]() | 692 KB | Display | ![]() |
PDBx/mmJSON format | ![]() | Tree view | ![]() | |
Others | ![]() |
-Validation report
Arichive directory | ![]() ![]() | HTTPS FTP |
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-Related structure data
Related structure data | |
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Similar structure data |
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Links
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Assembly
Deposited unit | ![]()
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2 | ![]()
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Unit cell |
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Components
-Fumarate reductase ... , 4 types, 8 molecules AMBNCODP
#1: Protein | Mass: 63477.707 Da / Num. of mol.: 2 Source method: isolated from a genetically manipulated source Source: (gene. exp.) ![]() ![]() ![]() ![]() ![]() ![]() ![]() #2: Protein | Mass: 27021.885 Da / Num. of mol.: 2 Source method: isolated from a genetically manipulated source Source: (gene. exp.) ![]() ![]() ![]() ![]() ![]() ![]() #3: Protein | ![]() Mass: 14882.818 Da / Num. of mol.: 2 / Mutation: E29L Source method: isolated from a genetically manipulated source Source: (gene. exp.) ![]() ![]() ![]() ![]() ![]() ![]() #4: Protein | ![]() Mass: 13118.870 Da / Num. of mol.: 2 Source method: isolated from a genetically manipulated source Source: (gene. exp.) ![]() ![]() ![]() ![]() ![]() ![]() |
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-Non-polymers , 5 types, 10 molecules ![](data/chem/img/FAD.gif)
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#5: Chemical | ![]() #6: Chemical | ![]() #7: Chemical | ![]() #8: Chemical | ![]() #9: Chemical | ![]() |
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-Experimental details
-Experiment
Experiment | Method: ![]() |
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Sample preparation
Crystal | Density Matthews: 3.88 Å3/Da / Density % sol: 68.3 % |
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Crystal grow![]() | Temperature: 295.15 K / Method: vapor diffusion, hanging drop / pH: 5.8 Details: 13%-15% PEG 5000 MME, 85mM-115mM Magnesium Acetate, 100mM Sodium Citrate, 0.01mM DTT, 0.1mM EDTA, pH 5.8, VAPOR DIFFUSION, HANGING DROP, temperature 295.15K |
-Data collection
Diffraction | Mean temperature: 295.15 K | |||||||||||||||||||||||||||||||||
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Diffraction source | Source: ![]() ![]() ![]() | |||||||||||||||||||||||||||||||||
Detector | Type: MAR scanner 300 mm plate / Detector: IMAGE PLATE / Date: Apr 7, 2012 | |||||||||||||||||||||||||||||||||
Radiation | Monochromator: 3.0 cm undulator using two Laue diamond monochromators and one 2.5 m large offset monochromator Protocol: SINGLE WAVELENGTH / Monochromatic (M) / Laue (L): M / Scattering type: x-ray | |||||||||||||||||||||||||||||||||
Radiation wavelength | Wavelength![]() | |||||||||||||||||||||||||||||||||
Reflection | Resolution: 2.95→47.8 Å / Num. obs: 75158 / % possible obs: 95.7 % / Observed criterion σ(F): 33.2 / Observed criterion σ(I): 116.3 | |||||||||||||||||||||||||||||||||
Reflection shell |
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Processing
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Refinement | Method to determine structure![]() ![]()
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Solvent computation | Ion probe radii: 0.8 Å / Shrinkage radii: 0.8 Å / VDW probe radii: 1.2 Å / Solvent model: BABINET MODEL WITH MASK | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Displacement parameters | Biso mean: 75.533 Å2
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Refinement step | Cycle: LAST / Resolution: 2.95→47.8 Å
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Refine LS restraints |
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