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- PDB-4k34: Crystal structures of CusC review conformational changes accompan... -
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Open data
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Basic information
Entry | Database: PDB / ID: 4k34 | ||||||
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Title | Crystal structures of CusC review conformational changes accompanying folding and transmembrane channel formation | ||||||
![]() | Cation efflux system protein CusC | ||||||
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Function / homology | ![]() protein palmitoylation / copper ion transmembrane transport / response to silver ion / silver ion transmembrane transport / ![]() ![]() ![]() ![]() ![]() Similarity search - Function | ||||||
Biological species | ![]() ![]() ![]() | ||||||
Method | ![]() ![]() ![]() ![]() | ||||||
![]() | Su, C.-C. | ||||||
![]() | ![]() Title: Crystal Structures of CusC Review Conformational Changes Accompanying Folding and Transmembrane Channel Formation. Authors: Lei, H.T. / Bolla, J.R. / Bishop, N.R. / Su, C.C. / Yu, E.W. | ||||||
History |
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Structure visualization
Structure viewer | Molecule: ![]() ![]() |
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Downloads & links
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Download
PDBx/mmCIF format | ![]() | 152.5 KB | Display | ![]() |
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PDB format | ![]() | 120.6 KB | Display | ![]() |
PDBx/mmJSON format | ![]() | Tree view | ![]() | |
Others | ![]() |
-Validation report
Arichive directory | ![]() ![]() | HTTPS FTP |
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-Related structure data
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Links
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Assembly
Deposited unit | ![]()
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2 | ![]()
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3 | ![]()
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Unit cell |
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Components
#1: Protein | Mass: 49375.914 Da / Num. of mol.: 2 / Mutation: C1S Source method: isolated from a genetically manipulated source Source: (gene. exp.) ![]() ![]() ![]() ![]() ![]() ![]() #2: Water | ChemComp-HOH / | ![]() |
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-Experimental details
-Experiment
Experiment | Method: ![]() |
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Sample preparation
Crystal | Density Matthews: 2.34 Å3/Da / Density % sol: 47.37 % |
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Crystal grow![]() | Temperature: 298 K / Method: vapor diffusion / pH: 7.5 Details: 12% PEG3350, 0.1M NaAc, 0.1M Na-Citric (6.5) and 15% 2-3 Butanediol, pH 7.5, VAPOR DIFFUSION, temperature 298K |
-Data collection
Diffraction | Mean temperature: 100 K |
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Diffraction source | Source: ![]() ![]() ![]() |
Detector | Type: ADSC QUANTUM 315r / Detector: CCD / Date: Feb 3, 2012 |
Radiation | Monochromator: Si(111) / Protocol: SINGLE WAVELENGTH / Monochromatic (M) / Laue (L): M / Scattering type: x-ray |
Radiation wavelength | Wavelength![]() |
Reflection | Resolution: 2.69→50 Å / Num. obs: 24838 / % possible obs: 98.5 % / Observed criterion σ(F): 2 / Observed criterion σ(I): 2 |
Reflection shell | Resolution: 2.69→2.84 Å / % possible all: 95 |
-Phasing
Phasing![]() | Method: ![]() |
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Processing
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Refinement | Method to determine structure![]() ![]()
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Solvent computation | Shrinkage radii: 0.98 Å / VDW probe radii: 1.2 Å / Solvent model: FLAT BULK SOLVENT MODEL / Bsol: 46.385 Å2 / ksol: 0.346 e/Å3 | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Displacement parameters | Biso max: 138.2 Å2 / Biso mean: 50.7801 Å2 / Biso min: 12.42 Å2
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Refinement step | Cycle: LAST / Resolution: 2.69→42.321 Å
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Refine LS restraints |
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LS refinement shell | Refine-ID: X-RAY DIFFRACTION / Total num. of bins used: 9
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