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- PDB-4jlv: Crystal structure of the chimerical protein CapA1B1 in complex wi... -
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Open data
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Basic information
Entry | Database: PDB / ID: 4jlv | ||||||
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Title | Crystal structure of the chimerical protein CapA1B1 in complex with ADP-Mg | ||||||
![]() | C-terminal fragment of Membrane protein CapA1, Putative uncharacterized protein capB1 | ||||||
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Function / homology | ![]() carbohydrate:proton symporter activity / polysaccharide transport / extracellular polysaccharide biosynthetic process / ![]() ![]() ![]() ![]() Similarity search - Function | ||||||
Biological species | ![]() ![]() ![]() | ||||||
Method | ![]() ![]() ![]() | ||||||
![]() | Gruszczyk, J. / Olivares-Illana, V. / Nourikyan, J. / Fleurie, A. / Bechet, E. / Aumont-Nicaise, M. / Gueguen-Chaignon, V. / Morera, S. / Grangeasse, C. / Nessler, S. | ||||||
![]() | ![]() Title: Comparative analysis of the Tyr-kinases CapB1 and CapB2 fused to their cognate modulators CapA1 and CapA2 from Staphylococcus aureus Authors: Gruszczyk, J. / Olivares-Illana, V. / Nourikyan, J. / Fleurie, A. / Bechet, E. / Gueguen-Chaignon, V. / Freton, C. / Aumont-Nicaise, M. / Morera, S. / Grangeasse, C. / Nessler, S. | ||||||
History |
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Structure visualization
Structure viewer | Molecule: ![]() ![]() |
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Downloads & links
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Download
PDBx/mmCIF format | ![]() | 60.5 KB | Display | ![]() |
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PDB format | ![]() | 41.9 KB | Display | ![]() |
PDBx/mmJSON format | ![]() | Tree view | ![]() | |
Others | ![]() |
-Validation report
Arichive directory | ![]() ![]() | HTTPS FTP |
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-Related structure data
Related structure data | ![]() 4jmpC ![]() 3bfvS S: Starting model for refinement C: citing same article ( |
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Similar structure data |
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Links
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Assembly
Deposited unit | ![]()
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Unit cell |
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Components
#1: Protein | Mass: 30096.195 Da / Num. of mol.: 1 Source method: isolated from a genetically manipulated source Details: Chimera of Membrane protein CapA1 and tyrosine kinase CapB1 Source: (gene. exp.) ![]() ![]() ![]() ![]() ![]() ![]() |
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#2: Chemical | ChemComp-ADP / ![]() |
#3: Chemical | ChemComp-MG / |
#4: Water | ChemComp-HOH / ![]() |
-Experimental details
-Experiment
Experiment | Method: ![]() |
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Sample preparation
Crystal | Density Matthews: 1.94 Å3/Da / Density % sol: 36.74 % |
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Crystal grow![]() | Temperature: 290 K / Method: vapor diffusion / pH: 8.5 Details: 28% PEG 1000, 100mM Tris-HCl, pH 8.5, VAPOR DIFFUSION, temperature 290K |
-Data collection
Diffraction | Mean temperature: 100 K |
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Diffraction source | Source: ![]() ![]() ![]() |
Detector | Type: ADSC QUANTUM 210 / Detector: CCD / Date: Aug 26, 2008 |
Radiation | Monochromator: cooled channel-cut Si(311) crystal / Protocol: SINGLE WAVELENGTH / Monochromatic (M) / Laue (L): M / Scattering type: x-ray |
Radiation wavelength | Wavelength![]() |
Reflection | Resolution: 2.2→50 Å / Num. obs: 12422 / % possible obs: 99.7 % / Observed criterion σ(F): 0 / Observed criterion σ(I): -3 / Redundancy: 4.752 % |
Reflection shell | Resolution: 2.2→2.33 Å / Redundancy: 4.81 % / Rmerge(I) obs: 1.113 / Mean I/σ(I) obs: 1.93 / Num. unique all: 1515 / Rsym value: 1.252 / % possible all: 99.5 |
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Processing
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Refinement | Method to determine structure![]() ![]() Starting model: 3BFV Resolution: 2.2→44.14 Å / Occupancy max: 1 / Occupancy min: 1 / FOM work R set: 0.7625 / SU ML: 0.25 / σ(F): 1.36 / Phase error: 29.1 / Stereochemistry target values: ML
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Solvent computation | Shrinkage radii: 0.9 Å / VDW probe radii: 1.11 Å / Solvent model: FLAT BULK SOLVENT MODEL | |||||||||||||||||||||||||||||||||||
Displacement parameters | Biso max: 91.71 Å2 / Biso mean: 57.5635 Å2 / Biso min: 35.93 Å2 | |||||||||||||||||||||||||||||||||||
Refinement step | Cycle: LAST / Resolution: 2.2→44.14 Å
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Refine LS restraints |
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LS refinement shell | Refine-ID: X-RAY DIFFRACTION / Total num. of bins used: 4
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