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- PDB-4irl: X-ray structure of the CARD domain of zebrafish GBP-NLRP1 like protein -

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Basic information

Entry
Database: PDB / ID: 4irl
TitleX-ray structure of the CARD domain of zebrafish GBP-NLRP1 like protein
ComponentsMaltose-binding periplasmic protein, Novel protein similar to vertebrate guanylate binding protein family
KeywordsAPOPTOSIS / CARD / death fold superfamily / six-helix bundle / inflammasome / innate immune system / signal transduction
Function / homology
Function and homology information


detection of maltose stimulus / maltose transport complex / maltose binding / maltose transport / maltodextrin transmembrane transport / carbohydrate transport / carbohydrate transmembrane transporter activity / ATP-binding cassette (ABC) transporter complex, substrate-binding subunit-containing / ATP-binding cassette (ABC) transporter complex / cell chemotaxis ...detection of maltose stimulus / maltose transport complex / maltose binding / maltose transport / maltodextrin transmembrane transport / carbohydrate transport / carbohydrate transmembrane transporter activity / ATP-binding cassette (ABC) transporter complex, substrate-binding subunit-containing / ATP-binding cassette (ABC) transporter complex / cell chemotaxis / outer membrane-bounded periplasmic space / regulation of apoptotic process / periplasmic space / inflammatory response / innate immune response / GTPase activity / DNA damage response / GTP binding / membrane / cytoplasm
Similarity search - Function
FIIND domain / Function to find / FIIND domain profile. / Guanylate-binding protein, C-terminal / Guanylate-binding protein/Atlastin, C-terminal / Guanylate-binding protein, C-terminal domain / CARD8/ASC/NALP1, CARD domain / Guanylate-binding protein, N-terminal / Guanylate-binding protein, C-terminal domain superfamily / Guanylate-binding protein, N-terminal domain ...FIIND domain / Function to find / FIIND domain profile. / Guanylate-binding protein, C-terminal / Guanylate-binding protein/Atlastin, C-terminal / Guanylate-binding protein, C-terminal domain / CARD8/ASC/NALP1, CARD domain / Guanylate-binding protein, N-terminal / Guanylate-binding protein, C-terminal domain superfamily / Guanylate-binding protein, N-terminal domain / GB1/RHD3-type guanine nucleotide-binding (G) domain / GB1/RHD3-type guanine nucleotide-binding (G) domain profile. / Death Domain, Fas / Death Domain, Fas / CARD domain / CARD caspase recruitment domain profile. / Caspase recruitment domain / Maltose/Cyclodextrin ABC transporter, substrate-binding protein / Solute-binding family 1, conserved site / Bacterial extracellular solute-binding proteins, family 1 signature. / Bacterial extracellular solute-binding protein / Bacterial extracellular solute-binding protein / Death-like domain superfamily / P-loop containing nucleoside triphosphate hydrolase / Orthogonal Bundle / Mainly Alpha
Similarity search - Domain/homology
alpha-maltotetraose / ACETATE ION / MALONATE ION / DI(HYDROXYETHYL)ETHER / Guanylate-binding protein 3 / Maltose/maltodextrin-binding periplasmic protein
Similarity search - Component
Biological speciesEscherichia coli (E. coli)
Danio rerio (zebrafish)
MethodX-RAY DIFFRACTION / SYNCHROTRON / MOLECULAR REPLACEMENT / Resolution: 1.47 Å
AuthorsJin, T. / Huang, M. / Smith, P. / Xiao, T.
CitationJournal: Acta Crystallogr.,Sect.F / Year: 2013
Title: Structure of the caspase-recruitment domain from a zebrafish guanylate-binding protein.
Authors: Jin, T. / Huang, M. / Smith, P. / Jiang, J. / Xiao, T.S.
History
DepositionJan 15, 2013Deposition site: RCSB / Processing site: RCSB
Revision 1.0Aug 7, 2013Provider: repository / Type: Initial release
Revision 1.1Aug 21, 2013Group: Database references
Revision 1.2Nov 26, 2014Group: Refinement description
Revision 1.3Aug 2, 2017Group: Refinement description / Source and taxonomy / Category: entity_src_gen / software
Revision 1.4Nov 15, 2017Group: Refinement description / Category: software / Item: _software.name
Revision 2.0Jul 29, 2020Group: Atomic model / Data collection ...Atomic model / Data collection / Database references / Derived calculations / Non-polymer description / Structure summary
Category: atom_site / chem_comp ...atom_site / chem_comp / entity / entity_name_com / pdbx_branch_scheme / pdbx_chem_comp_identifier / pdbx_entity_branch / pdbx_entity_branch_descriptor / pdbx_entity_branch_link / pdbx_entity_branch_list / pdbx_entity_nonpoly / pdbx_molecule_features / pdbx_nonpoly_scheme / pdbx_struct_assembly_gen / pdbx_struct_conn_angle / struct_asym / struct_conn / struct_conn_type / struct_ref_seq_dif / struct_site / struct_site_gen
Item: _atom_site.B_iso_or_equiv / _atom_site.Cartn_x ..._atom_site.B_iso_or_equiv / _atom_site.Cartn_x / _atom_site.Cartn_y / _atom_site.Cartn_z / _atom_site.auth_asym_id / _atom_site.auth_atom_id / _atom_site.auth_comp_id / _atom_site.auth_seq_id / _atom_site.label_asym_id / _atom_site.label_atom_id / _atom_site.label_comp_id / _atom_site.label_entity_id / _atom_site.pdbx_formal_charge / _atom_site.type_symbol / _chem_comp.formula / _chem_comp.formula_weight / _chem_comp.id / _chem_comp.mon_nstd_flag / _chem_comp.name / _chem_comp.type / _entity.pdbx_description / _entity.src_method / _entity.type / _pdbx_struct_assembly_gen.asym_id_list / _pdbx_struct_conn_angle.ptnr1_auth_comp_id / _pdbx_struct_conn_angle.ptnr1_auth_seq_id / _pdbx_struct_conn_angle.ptnr1_label_asym_id / _pdbx_struct_conn_angle.ptnr1_label_atom_id / _pdbx_struct_conn_angle.ptnr1_label_comp_id / _pdbx_struct_conn_angle.ptnr1_label_seq_id / _pdbx_struct_conn_angle.ptnr2_auth_seq_id / _pdbx_struct_conn_angle.ptnr2_label_asym_id / _pdbx_struct_conn_angle.ptnr3_auth_comp_id / _pdbx_struct_conn_angle.ptnr3_auth_seq_id / _pdbx_struct_conn_angle.ptnr3_label_asym_id / _pdbx_struct_conn_angle.ptnr3_label_atom_id / _pdbx_struct_conn_angle.ptnr3_label_comp_id / _pdbx_struct_conn_angle.ptnr3_label_seq_id / _pdbx_struct_conn_angle.value / _struct_asym.entity_id / _struct_ref_seq_dif.details
Description: Carbohydrate remediation / Provider: repository / Type: Remediation
Revision 2.1Feb 28, 2024Group: Data collection / Database references / Structure summary
Category: chem_comp / chem_comp_atom ...chem_comp / chem_comp_atom / chem_comp_bond / database_2
Item: _chem_comp.pdbx_synonyms / _database_2.pdbx_DOI / _database_2.pdbx_database_accession

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Structure visualization

Structure viewerMolecule:
MolmilJmol/JSmol

Downloads & links

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Assembly

Deposited unit
A: Maltose-binding periplasmic protein, Novel protein similar to vertebrate guanylate binding protein family
B: Maltose-binding periplasmic protein, Novel protein similar to vertebrate guanylate binding protein family
C: Maltose-binding periplasmic protein, Novel protein similar to vertebrate guanylate binding protein family
hetero molecules


Theoretical massNumber of molelcules
Total (without water)159,94622
Polymers156,9543
Non-polymers2,99119
Water34,6251922
1
A: Maltose-binding periplasmic protein, Novel protein similar to vertebrate guanylate binding protein family
hetero molecules


Theoretical massNumber of molelcules
Total (without water)53,2316
Polymers52,3181
Non-polymers9135
Water181
TypeNameSymmetry operationNumber
identity operation1_555x,y,z1
MethodPISA
2
B: Maltose-binding periplasmic protein, Novel protein similar to vertebrate guanylate binding protein family
hetero molecules


Theoretical massNumber of molelcules
Total (without water)53,3789
Polymers52,3181
Non-polymers1,0608
Water181
TypeNameSymmetry operationNumber
identity operation1_555x,y,z1
MethodPISA
3
C: Maltose-binding periplasmic protein, Novel protein similar to vertebrate guanylate binding protein family
hetero molecules


Theoretical massNumber of molelcules
Total (without water)53,3377
Polymers52,3181
Non-polymers1,0196
Water181
TypeNameSymmetry operationNumber
identity operation1_555x,y,z1
MethodPISA
Unit cell
Length a, b, c (Å)65.749, 124.750, 178.044
Angle α, β, γ (deg.)90.00, 90.00, 90.00
Int Tables number19
Space group name H-MP212121

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Components

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Protein / Sugars , 2 types, 6 molecules ABC

#1: Protein Maltose-binding periplasmic protein, Novel protein similar to vertebrate guanylate binding protein family / MBP / MMBP / Maltodextrin-binding protein / Uncharacterized protein


Mass: 52318.109 Da / Num. of mol.: 3 / Fragment: Zebrafish GBP-NLRP1 CARD domain
Source method: isolated from a genetically manipulated source
Source: (gene. exp.) Escherichia coli (E. coli), (gene. exp.) Danio rerio (zebrafish)
Strain: K12
Gene: b4034, GBP-NLRP1, JW3994, malE, CH211-195H23.5-001, gbp3
Plasmid: pET30a / Production host: Escherichia coli (E. coli) / Strain (production host): BL21 / References: UniProt: P0AEX9, UniProt: B0V1H4
#2: Polysaccharide alpha-D-glucopyranose-(1-4)-alpha-D-glucopyranose-(1-4)-alpha-D-glucopyranose-(1-4)-alpha-D-glucopyranose / alpha-maltotetraose


Type: oligosaccharide, Oligosaccharide / Class: Substrate analog / Mass: 666.578 Da / Num. of mol.: 3
Source method: isolated from a genetically manipulated source
Details: oligosaccharide / References: alpha-maltotetraose
DescriptorTypeProgram
DGlcpa1-4DGlcpa1-4DGlcpa1-4DGlcpa1-ROHGlycam Condensed SequenceGMML 1.0
WURCS=2.0/1,4,3/[a2122h-1a_1-5]/1-1-1-1/a4-b1_b4-c1_c4-d1WURCSPDB2Glycan 1.1.0
[][a-D-Glcp]{[(4+1)][a-D-Glcp]{[(4+1)][a-D-Glcp]{[(4+1)][a-D-Glcp]{}}}}LINUCSPDB-CARE

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Non-polymers , 6 types, 1938 molecules

#3: Chemical ChemComp-MLI / MALONATE ION / Malonic acid


Mass: 102.046 Da / Num. of mol.: 3 / Source method: obtained synthetically / Formula: C3H2O4
#4: Chemical
ChemComp-EDO / 1,2-ETHANEDIOL / ETHYLENE GLYCOL / Ethylene glycol


Mass: 62.068 Da / Num. of mol.: 5 / Source method: obtained synthetically / Formula: C2H6O2
#5: Chemical ChemComp-ACT / ACETATE ION / Acetate


Mass: 59.044 Da / Num. of mol.: 3 / Source method: obtained synthetically / Formula: C2H3O2
#6: Chemical
ChemComp-NA / SODIUM ION


Mass: 22.990 Da / Num. of mol.: 4 / Source method: obtained synthetically / Formula: Na
#7: Chemical ChemComp-PEG / DI(HYDROXYETHYL)ETHER / Diethylene glycol


Mass: 106.120 Da / Num. of mol.: 1 / Source method: obtained synthetically / Formula: C4H10O3
#8: Water ChemComp-HOH / water / Water


Mass: 18.015 Da / Num. of mol.: 1922 / Source method: isolated from a natural source / Formula: H2O

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Experimental details

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Experiment

ExperimentMethod: X-RAY DIFFRACTION / Number of used crystals: 1

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Sample preparation

CrystalDensity Matthews: 2.33 Å3/Da / Density % sol: 47.12 %
Crystal growTemperature: 298 K / Method: vapor diffusion, hanging drop / pH: 6
Details: 20% PEG1000, 0.2 M Sodium Malonate, 0.1 M MES 6.0, VAPOR DIFFUSION, HANGING DROP, temperature 298K

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Data collection

Diffraction sourceSource: SYNCHROTRON / Site: APS / Beamline: 23-ID-B / Wavelength: 0.97 Å
DetectorType: MARMOSAIC 300 mm CCD / Detector: CCD / Date: Nov 20, 2012
RadiationMonochromator: Si 111 CHANNEL / Protocol: SINGLE WAVELENGTH / Monochromatic (M) / Laue (L): M / Scattering type: x-ray
Radiation wavelengthWavelength: 0.97 Å / Relative weight: 1
ReflectionResolution: 1.447→50 Å / Num. all: 260018 / Num. obs: 243247 / % possible obs: 93.55 % / Observed criterion σ(I): 1.5 / Redundancy: 12.9 % / Biso Wilson estimate: 14.2 Å2 / Net I/σ(I): 8.17

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Processing

Software
NameVersionClassification
Blu-Icedata collection
PHASERphasing
PHENIX(phenix.refine: dev_1217)refinement
XDSdata reduction
XDSdata scaling
RefinementMethod to determine structure: MOLECULAR REPLACEMENT / Resolution: 1.47→29.434 Å / SU ML: 0.18 / σ(F): 1.34 / Phase error: 24.15 / Stereochemistry target values: ML
RfactorNum. reflection% reflection
Rfree0.2239 11928 5.02 %
Rwork0.1881 --
obs0.1899 237398 95.62 %
all-260018 -
Solvent computationShrinkage radii: 0.9 Å / VDW probe radii: 1.11 Å / Solvent model: FLAT BULK SOLVENT MODEL
Refinement stepCycle: LAST / Resolution: 1.47→29.434 Å
ProteinNucleic acidLigandSolventTotal
Num. atoms10716 0 199 1922 12837
Refine LS restraints
Refine-IDTypeDev idealNumber
X-RAY DIFFRACTIONf_bond_d0.0111321
X-RAY DIFFRACTIONf_angle_d1.29415391
X-RAY DIFFRACTIONf_dihedral_angle_d13.1074153
X-RAY DIFFRACTIONf_chiral_restr0.0851727
X-RAY DIFFRACTIONf_plane_restr0.0061978
LS refinement shell
Resolution (Å)Rfactor RfreeNum. reflection RfreeRfactor RworkNum. reflection RworkRefine-ID% reflection obs (%)
1.47-1.48670.31452860.27385285X-RAY DIFFRACTION68
1.4867-1.50420.30033140.26875621X-RAY DIFFRACTION73
1.5042-1.52250.30542750.26095968X-RAY DIFFRACTION76
1.5225-1.54180.28083140.26326312X-RAY DIFFRACTION81
1.5418-1.56210.30893500.25126691X-RAY DIFFRACTION86
1.5621-1.58350.29813660.23487097X-RAY DIFFRACTION91
1.5835-1.60610.25593990.23887476X-RAY DIFFRACTION96
1.6061-1.63010.27474050.22637654X-RAY DIFFRACTION99
1.6301-1.65560.26813690.21917820X-RAY DIFFRACTION100
1.6556-1.68270.25144070.2097845X-RAY DIFFRACTION100
1.6827-1.71170.26214240.20327769X-RAY DIFFRACTION100
1.7117-1.74280.24813970.20187820X-RAY DIFFRACTION100
1.7428-1.77640.23654310.20227841X-RAY DIFFRACTION100
1.7764-1.81260.2314210.2047781X-RAY DIFFRACTION100
1.8126-1.8520.23564210.27771X-RAY DIFFRACTION100
1.852-1.89510.25243940.19747878X-RAY DIFFRACTION100
1.8951-1.94250.24264230.20147822X-RAY DIFFRACTION100
1.9425-1.9950.23834320.20287831X-RAY DIFFRACTION100
1.995-2.05370.23164210.19517828X-RAY DIFFRACTION100
2.0537-2.11990.22594230.19697846X-RAY DIFFRACTION100
2.1199-2.19570.23824240.19027835X-RAY DIFFRACTION100
2.1957-2.28360.23494260.19097850X-RAY DIFFRACTION100
2.2836-2.38740.22064460.18647839X-RAY DIFFRACTION100
2.3874-2.51330.22274060.19047906X-RAY DIFFRACTION100
2.5133-2.67060.23994200.19157892X-RAY DIFFRACTION100
2.6706-2.87670.23284390.18417946X-RAY DIFFRACTION100
2.8767-3.16580.21124260.17957895X-RAY DIFFRACTION100
3.1658-3.62320.19923920.16278027X-RAY DIFFRACTION100
3.6232-4.56220.17034400.14458027X-RAY DIFFRACTION100
4.5622-29.43940.18064370.16738297X-RAY DIFFRACTION99
Refinement TLS params.

Method: refined / Refine-ID: X-RAY DIFFRACTION

IDL11 (°2)L12 (°2)L13 (°2)L22 (°2)L23 (°2)L33 (°2)S11 (Å °)S12 (Å °)S13 (Å °)S21 (Å °)S22 (Å °)S23 (Å °)S31 (Å °)S32 (Å °)S33 (Å °)T11 (Å2)T12 (Å2)T13 (Å2)T22 (Å2)T23 (Å2)T33 (Å2)Origin x (Å)Origin y (Å)Origin z (Å)
12.3840.0586-0.09672.96590.60282.4584-0.06490.11560.0662-0.25590.0515-0.0238-0.105-0.11690.00560.15360.00490.00630.07360.02550.0856-26.823138.8060.7158
22.9993-1.33060.1322.31180.26722.1146-0.0374-0.0134-0.1457-0.0641-0.01490.21260.05-0.15940.0440.12210.01420.00760.0841-0.01980.0867-36.458431.946912.5383
30.85120.1076-0.38620.50760.16960.77060.0665-0.1610.06110.0558-0.0159-0.0294-0.02710.0868-0.05510.14440.0314-0.01050.12350.00190.0942-25.911447.410933.3556
40.73150.38510.35340.55540.5871.3367-0.0005-0.0531-0.01620.0036-0.0056-0.01990.03080.01140.00550.11980.0499-0.0110.08040.00660.0881-22.98339.559420.4635
51.68221.41230.38482.22840.7083.3609-0.0677-0.1173-0.14470.01870.0569-0.35850.13410.32420.01410.11470.05650.00730.1285-0.01340.1383-16.770530.623313.8302
62.4922-0.0312-0.28010.65630.83571.21510.066-0.0061-0.0617-0.0372-0.07490.0911-0.0884-0.01980.01030.1250.01750.01990.08610.03150.0514-32.999242.998728.1882
76.78756.31746.43476.16946.11676.15380.0841-0.20690.147-0.0093-0.22550.215-0.0621-0.32170.19270.12840.03770.01040.1401-0.01660.1206-49.049644.303429.2798
83.365-0.0349-0.84723.955-1.31353.712-0.03590.05860.2849-0.3839-0.0689-0.2761-0.35980.05360.08980.2640.012-0.01870.1125-0.02850.1379-58.778747.46229.2244
94.60080.3380.05310.69141.57584.497-0.0835-0.3841-0.13260.0137-0.0035-0.00470.1628-0.05670.07690.19440.0604-0.01490.09120.01040.1352-59.712542.152216.7342
102.1407-0.3316-0.62823.6621.35612.5877-0.08390.12410.1203-0.20190.0792-0.0234-0.1112-0.0507-0.0280.14960.0207-0.0130.07950.00140.0917-25.8641-2.54740.0628
110.37010.26140.16950.380.16140.7563-0.0185-0.0084-0.00250.0345-0.00770.03190.13460.03420.02070.17660.0839-0.01070.1102-0.02540.1184-29.7829-6.345816.6808
122.78891.8992-0.45034.2576-0.0371.93310.1674-0.1580.0520.0156-0.1373-0.2201-0.2132-0.0167-0.02840.11670.0570.01160.1042-0.02190.0953-20.57711.157534.7895
133.87031.5161-0.63680.7944-0.39731.26460.0375-0.1603-0.1423-0.0689-0.1309-0.0534-0.0267-0.11940.08020.14360.0888-0.02450.1141-0.01290.1076-31.32016.6933.1833
140.45710.33170.43040.28740.29960.87560.0187-0.0223-0.04610.0124-0.0144-0.04160.02660.007-0.00980.12780.0671-0.01550.1057-0.00680.0961-21.7075-1.189620.1984
150.64820.52850.5460.55390.14681.1496-0.1032-0.1065-0.08550.10610.1178-0.2530.20810.33480.02360.22410.1501-0.04780.2242-0.06580.186-15.4564-10.490113.6491
161.72150.79920.69820.39330.4210.73450.037-0.12410.05-0.0497-0.11260.1155-0.0367-0.09530.07010.1740.0807-0.02420.1616-0.02120.1061-37.0163.071428.1495
172.45751.0015-0.78655.0357-1.41254.1830.00710.02710.2966-0.07790.0267-0.1216-0.10190.0671-0.0390.1060.0337-0.01170.0722-0.02730.1445-57.67766.44967.9717
183.93150.3605-0.05130.98841.64193.2574-0.0927-0.4369-0.05470.14310.0623-0.02410.20250.07180.03140.15340.0508-0.02580.07870.00050.1394-58.56610.964216.2996
191.4912-0.6379-0.44543.11111.26771.4786-0.0720.03630.1356-0.3301-0.020.0902-0.3348-0.10570.05050.21790.0237-0.02050.0767-0.00730.108-25.4904-44.3820.6899
202.1289-0.90750.09892.32880.52631.2922-0.1003-0.2239-0.0105-0.0187-0.00020.1887-0.0808-0.26470.07590.13480.0396-0.0070.1525-0.03370.099-35.0468-50.80712.7455
210.22760.44890.12211.88891.50311.6092-0.0251-0.1748-0.0042-0.06990.0118-0.1253-0.1365-0.10670.0120.11350.0524-0.00040.1439-0.00030.093-15.2043-40.396829.4832
221.51690.39950.1862.13920.77651.24280.24340.03520.1019-0.172-0.2034-0.1252-0.2846-0.251-0.04120.16280.07960.00810.2336-0.03950.0802-20.7049-30.153834.7472
231.28490.05310.32680.62220.57991.28150.2331-0.0626-0.169-0.1497-0.3620.0989-0.0028-0.49550.12250.20650.1525-0.02480.2834-0.10570.1596-31.2465-34.399733.2056
240.18570.28040.40920.61490.81521.3218-0.0109-0.0705-0.0228-0.0252-0.0008-0.03540.0218-0.06020.02110.12670.05740.00140.1289-0.00890.0903-21.6633-42.776720.3457
251.55221.79081.3752.36031.8093.6244-0.014-0.1571-0.15770.0760.0876-0.23160.16220.2277-0.05270.11070.06060.01410.1407-0.01090.1275-15.491-51.984314.2763
260.29260.042-0.17850.48750.14240.1581-0.0774-0.25850.0597-0.026-0.11790.0936-0.1076-0.3293-0.01140.19960.1633-0.050.4221-0.16060.1551-37.0203-38.588628.2223
271.00180.1543-0.07474.0153-1.30854.92390.0101-0.15040.3721-0.1879-0.0279-0.3441-0.24320.12480.01210.1040.02880.03060.1177-0.06590.255-57.4444-35.01768.3844
284.25910.3329-0.26920.79971.51093.116-0.1245-0.66630.06270.18660.1004-0.12210.28850.19050.0140.16490.0724-0.03440.1889-0.05170.1646-58.797-40.477316.656
Refinement TLS group
IDRefine-IDRefine TLS-IDSelection details
1X-RAY DIFFRACTION1chain 'A' and (resid 1 through 43 )
2X-RAY DIFFRACTION2chain 'A' and (resid 44 through 106 )
3X-RAY DIFFRACTION3chain 'A' and (resid 107 through 219 )
4X-RAY DIFFRACTION4chain 'A' and (resid 220 through 283 )
5X-RAY DIFFRACTION5chain 'A' and (resid 284 through 315 )
6X-RAY DIFFRACTION6chain 'A' and (resid 316 through 357 )
7X-RAY DIFFRACTION7chain 'A' and (resid 358 through 381 )
8X-RAY DIFFRACTION8chain 'A' and (resid 382 through 419 )
9X-RAY DIFFRACTION9chain 'A' and (resid 420 through 462 )
10X-RAY DIFFRACTION10chain 'B' and (resid 1 through 43 )
11X-RAY DIFFRACTION11chain 'B' and (resid 44 through 129 )
12X-RAY DIFFRACTION12chain 'B' and (resid 130 through 164 )
13X-RAY DIFFRACTION13chain 'B' and (resid 165 through 219 )
14X-RAY DIFFRACTION14chain 'B' and (resid 220 through 283 )
15X-RAY DIFFRACTION15chain 'B' and (resid 284 through 315 )
16X-RAY DIFFRACTION16chain 'B' and (resid 316 through 381 )
17X-RAY DIFFRACTION17chain 'B' and (resid 382 through 419 )
18X-RAY DIFFRACTION18chain 'B' and (resid 420 through 463 )
19X-RAY DIFFRACTION19chain 'C' and (resid 1 through 43 )
20X-RAY DIFFRACTION20chain 'C' and (resid 44 through 106 )
21X-RAY DIFFRACTION21chain 'C' and (resid 107 through 129 )
22X-RAY DIFFRACTION22chain 'C' and (resid 130 through 164 )
23X-RAY DIFFRACTION23chain 'C' and (resid 165 through 219 )
24X-RAY DIFFRACTION24chain 'C' and (resid 220 through 283 )
25X-RAY DIFFRACTION25chain 'C' and (resid 284 through 315 )
26X-RAY DIFFRACTION26chain 'C' and (resid 316 through 381 )
27X-RAY DIFFRACTION27chain 'C' and (resid 382 through 419 )
28X-RAY DIFFRACTION28chain 'C' and (resid 420 through 462 )

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