- PDB-4ilv: Structure of the dioxygenase domain of SACTE_2871, a novel dioxyg... -
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Basic information
Entry
Database: PDB / ID: 4ilv
Title
Structure of the dioxygenase domain of SACTE_2871, a novel dioxygenase carbohydrate-binding protein fusion from the cellulolytic bacterium Streptomyces sp. SirexAA-E
Components
Intradiol ring-cleavage dioxygenase
Keywords
OXIDOREDUCTASE / iron center / intradiol dioxygenase / carbohydrate binding domain
Function / homology
Function and homology information
: / oxidoreductase activity, acting on single donors with incorporation of molecular oxygen, incorporation of two atoms of oxygen / hydrolase activity, hydrolyzing O-glycosyl compounds / ferric iron binding / carbohydrate binding / carbohydrate metabolic process / extracellular region Similarity search - Function
Carbohydrate-binding module family 5/12 / Protocatechuate 3,4-Dioxygenase, subunit A / Aromatic compound dioxygenase / Intradiol ring-cleavage dioxygenase, C-terminal / Intradiol ring-cleavage dioxygenase, core / Dioxygenase / Chitin-binding domain type 3 / Carbohydrate-binding module family 5/12 / Carbohydrate-binding module superfamily 5/12 / Twin arginine translocation (Tat) signal profile. ...Carbohydrate-binding module family 5/12 / Protocatechuate 3,4-Dioxygenase, subunit A / Aromatic compound dioxygenase / Intradiol ring-cleavage dioxygenase, C-terminal / Intradiol ring-cleavage dioxygenase, core / Dioxygenase / Chitin-binding domain type 3 / Carbohydrate-binding module family 5/12 / Carbohydrate-binding module superfamily 5/12 / Twin arginine translocation (Tat) signal profile. / Twin-arginine translocation pathway, signal sequence / Sandwich / Mainly Beta Similarity search - Domain/homology
Mass: 18.015 Da / Num. of mol.: 198 / Source method: isolated from a natural source / Formula: H2O
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Experimental details
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Experiment
Experiment
Method: X-RAY DIFFRACTION / Number of used crystals: 1
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Sample preparation
Crystal
Density Matthews: 1.84 Å3/Da / Density % sol: 33.33 %
Crystal grow
Temperature: 298 K / Method: vapor diffusion, hanging drop Details: Protein Solution (10 mg/ml protein, 0.05 M NaCl, 0.010 M MOPS pH 7.0) mixed in a 1:1 ratio with the Well Solution (16% Polyethylene glycol 3350, 200mM Sodium Malonate, 100mM Bis-Tris pH 5.5). ...Details: Protein Solution (10 mg/ml protein, 0.05 M NaCl, 0.010 M MOPS pH 7.0) mixed in a 1:1 ratio with the Well Solution (16% Polyethylene glycol 3350, 200mM Sodium Malonate, 100mM Bis-Tris pH 5.5). Cryoprotected with 16% Polyethylene glycol 3350, 200mM Sodium Malonate, 15% ethylene glycol, 100mM Bis-Tris pH 5.5, vapor diffusion, hanging drop, temperature 298K
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