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Yorodumi- PDB-4htp: Crystal structure of the DBD domain of human DNA ligase IV bound ... -
+Open data
-Basic information
Entry | Database: PDB / ID: 4htp | ||||||
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Title | Crystal structure of the DBD domain of human DNA ligase IV bound to Artemis peptide | ||||||
Components |
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Keywords | LIGASE/HYDROLASE / Helical domain / DNA binding domain / DNA / artemis / LIGASE-HYDROLASE complex | ||||||
Function / homology | Function and homology information DNA ligation involved in DNA recombination / positive regulation of chromosome organization / DNA ligase IV complex / DNA ligation involved in DNA repair / DNA ligase activity / DN2 thymocyte differentiation / DNA ligase (ATP) / T cell receptor V(D)J recombination / pro-B cell differentiation / DNA ligase (ATP) activity ...DNA ligation involved in DNA recombination / positive regulation of chromosome organization / DNA ligase IV complex / DNA ligation involved in DNA repair / DNA ligase activity / DN2 thymocyte differentiation / DNA ligase (ATP) / T cell receptor V(D)J recombination / pro-B cell differentiation / DNA ligase (ATP) activity / single-stranded DNA endodeoxyribonuclease activity / DNA-dependent protein kinase-DNA ligase 4 complex / single strand break repair / immunoglobulin V(D)J recombination / nonhomologous end joining complex / 5'-3' exonuclease activity / DNA ligation / V(D)J recombination / double-strand break repair via classical nonhomologous end joining / isotype switching / nucleotide-excision repair, DNA gap filling / positive regulation of neurogenesis / 5'-3' DNA exonuclease activity / response to ionizing radiation / DNA biosynthetic process / cellular response to lithium ion / 2-LTR circle formation / somatic stem cell population maintenance / ligase activity / response to X-ray / chromosome organization / interstrand cross-link repair / condensed chromosome / telomere maintenance / neurogenesis / B cell differentiation / central nervous system development / stem cell proliferation / cellular response to ionizing radiation / response to gamma radiation / Nonhomologous End-Joining (NHEJ) / double-strand break repair via nonhomologous end joining / establishment of integrated proviral latency / double-strand break repair / positive regulation of fibroblast proliferation / T cell differentiation in thymus / fibroblast proliferation / endonuclease activity / neuron apoptotic process / in utero embryonic development / adaptive immune response / negative regulation of neuron apoptotic process / cell population proliferation / damaged DNA binding / chromosome, telomeric region / Hydrolases; Acting on ester bonds / cell cycle / cell division / intracellular membrane-bounded organelle / Golgi apparatus / magnesium ion binding / DNA binding / nucleoplasm / ATP binding / nucleus Similarity search - Function | ||||||
Biological species | Homo sapiens (human) | ||||||
Method | X-RAY DIFFRACTION / SYNCHROTRON / MOLECULAR REPLACEMENT / Resolution: 2.2502 Å | ||||||
Authors | De Ioannes, P.E. / Aggarwal, A.K. | ||||||
Citation | Journal: Cell Rep / Year: 2012 Title: Structural Basis of DNA Ligase IV-Artemis Interaction in Nonhomologous End-Joining. Authors: De Ioannes, P. / Malu, S. / Cortes, P. / Aggarwal, A.K. | ||||||
History |
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-Structure visualization
Structure viewer | Molecule: MolmilJmol/JSmol |
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-Downloads & links
-Download
PDBx/mmCIF format | 4htp.cif.gz | 108.2 KB | Display | PDBx/mmCIF format |
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PDB format | pdb4htp.ent.gz | 81.6 KB | Display | PDB format |
PDBx/mmJSON format | 4htp.json.gz | Tree view | PDBx/mmJSON format | |
Others | Other downloads |
-Validation report
Arichive directory | https://data.pdbj.org/pub/pdb/validation_reports/ht/4htp ftp://data.pdbj.org/pub/pdb/validation_reports/ht/4htp | HTTPS FTP |
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-Related structure data
Related structure data | 4htoSC S: Starting model for refinement C: citing same article (ref.) |
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Similar structure data |
-Links
-Assembly
Deposited unit |
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2 |
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Unit cell |
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-Components
#1: Protein | Mass: 27345.660 Da / Num. of mol.: 2 / Fragment: DNA binding domain Source method: isolated from a genetically manipulated source Source: (gene. exp.) Homo sapiens (human) / Gene: LIG4 / Production host: Escherichia coli (E. coli) / Strain (production host): BL21(DE3) CP RIPL / References: UniProt: P49917, DNA ligase (ATP) #2: Protein/peptide | Mass: 1452.653 Da / Num. of mol.: 2 / Fragment: C-terminal / Source method: obtained synthetically / Details: This sequence occurs naturally in humans / Source: (synth.) Homo sapiens (human) References: UniProt: Q96SD1, Hydrolases; Acting on ester bonds #3: Water | ChemComp-HOH / | |
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-Experimental details
-Experiment
Experiment | Method: X-RAY DIFFRACTION / Number of used crystals: 1 |
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-Sample preparation
Crystal | Density Matthews: 3.06 Å3/Da / Density % sol: 59.78 % |
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Crystal grow | Temperature: 293.15 K / Method: vapor diffusion, hanging drop / pH: 8 Details: 18% PEG 1000, 200 mM Tris-HCl pH 8.0, VAPOR DIFFUSION, HANGING DROP, temperature 293.15K |
-Data collection
Diffraction | Mean temperature: 90 K | |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
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Diffraction source | Source: SYNCHROTRON / Site: NSLS / Beamline: X6A / Wavelength: 0.9394 Å | |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Detector | Type: ADSC QUANTUM 270 / Detector: CCD / Date: Mar 9, 2010 / Details: bending magnet | |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Radiation | Monochromator: Toroidal focusing mirror / Protocol: SINGLE WAVELENGTH / Monochromatic (M) / Laue (L): M / Scattering type: x-ray | |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Radiation wavelength | Wavelength: 0.9394 Å / Relative weight: 1 | |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Reflection | Resolution: 2.25→40 Å / Num. all: 32066 / Num. obs: 24874 / % possible obs: 91.8 % / Observed criterion σ(F): 1 / Observed criterion σ(I): 2 / Redundancy: 12.4 % / Rmerge(I) obs: 0.115 / Rsym value: 0.115 / Net I/σ(I): 31.6 | |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Reflection shell | Diffraction-ID: 1
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-Processing
Software |
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Refinement | Method to determine structure: MOLECULAR REPLACEMENT Starting model: PDB ENTRY 4HTO Resolution: 2.2502→33.073 Å / SU ML: 0.26 / σ(F): 1 / σ(I): 2 / Phase error: 26.71 / Stereochemistry target values: ML
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Solvent computation | Shrinkage radii: 0.9 Å / VDW probe radii: 1.11 Å / Solvent model: FLAT BULK SOLVENT MODEL | |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Refinement step | Cycle: LAST / Resolution: 2.2502→33.073 Å
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Refine LS restraints |
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LS refinement shell |
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