PDB-4hsn: Crystal structure of DAH7PS from Neisseria meningitidis

ID or keywords:

Entry

Database: PDB / ID: 4hsn

Title

Crystal structure of DAH7PS from Neisseria meningitidis

Components

3-deoxy-D-arabino-heptulosonate 7-phosphate synthase

Keywords

TRANSFERASE / DAHP / DAHPS / DAH7PS /

TIM BARREL /

ALLOSTERIC REGULATION / AROMATIC BIOSYNTHESIS /

SHIKIMATE PATHWAY

Function / homology

Function and homology information

3-deoxy-7-phosphoheptulonate synthase /

3-deoxy-7-phosphoheptulonate synthase activity / chorismate biosynthetic process / aromatic amino acid family biosynthetic process / amino acid biosynthetic process /

metal ion binding /

cytoplasm
Similarity search - Function

Biological species

Neisseria meningitidis (bacteria)

Method

X-RAY DIFFRACTION /

SYNCHROTRON /

MOLECULAR REPLACEMENT /

molecular replacement / Resolution: 2 Å

Authors

Cross, P.J. / Pietersma, A.L. / Allison, T.M. / Wilson-Coutts, S.M. / Cochrane, F.C. / Parker, E.J.

Citation

Journal: Protein Sci. / Year: 2013
Title: Neisseria meningitidis expresses a single 3-deoxy-d-arabino-heptulosonate 7-phosphate synthase that is inhibited primarily by phenylalanine.
Authors: Cross, P.J. / Pietersma, A.L. / Allison, T.M. / Wilson-Coutts, S.M. / Cochrane, F.C. / Parker, E.J.

History

Deposition	Oct 30, 2012	Deposition site: RCSB / Processing site: RCSB
Revision 1.0	Sep 11, 2013	Provider: repository / Type: Initial release
Revision 1.1	Sep 20, 2023	Group: Data collection / Database references ...Data collection / Database references / Derived calculations / Refinement description Category: chem_comp_atom / chem_comp_bond ...chem_comp_atom / chem_comp_bond / database_2 / pdbx_initial_refinement_model / pdbx_struct_conn_angle / struct_conn / struct_ncs_dom_lim / struct_site Item: _database_2.pdbx_DOI / _database_2.pdbx_database_accession ..._database_2.pdbx_DOI / _database_2.pdbx_database_accession / _pdbx_struct_conn_angle.ptnr1_auth_asym_id / _pdbx_struct_conn_angle.ptnr1_auth_comp_id / _pdbx_struct_conn_angle.ptnr1_auth_seq_id / _pdbx_struct_conn_angle.ptnr1_label_asym_id / _pdbx_struct_conn_angle.ptnr1_label_atom_id / _pdbx_struct_conn_angle.ptnr1_label_comp_id / _pdbx_struct_conn_angle.ptnr1_label_seq_id / _pdbx_struct_conn_angle.ptnr2_auth_asym_id / _pdbx_struct_conn_angle.ptnr2_label_asym_id / _pdbx_struct_conn_angle.ptnr3_auth_asym_id / _pdbx_struct_conn_angle.ptnr3_auth_comp_id / _pdbx_struct_conn_angle.ptnr3_auth_seq_id / _pdbx_struct_conn_angle.ptnr3_label_asym_id / _pdbx_struct_conn_angle.ptnr3_label_atom_id / _pdbx_struct_conn_angle.ptnr3_label_comp_id / _pdbx_struct_conn_angle.ptnr3_label_seq_id / _pdbx_struct_conn_angle.value / _struct_conn.pdbx_dist_value / _struct_conn.ptnr1_auth_asym_id / _struct_conn.ptnr1_auth_comp_id / _struct_conn.ptnr1_auth_seq_id / _struct_conn.ptnr1_label_asym_id / _struct_conn.ptnr1_label_atom_id / _struct_conn.ptnr1_label_comp_id / _struct_conn.ptnr1_label_seq_id / _struct_conn.ptnr2_auth_asym_id / _struct_conn.ptnr2_auth_comp_id / _struct_conn.ptnr2_auth_seq_id / _struct_conn.ptnr2_label_asym_id / _struct_conn.ptnr2_label_atom_id / _struct_conn.ptnr2_label_comp_id / _struct_ncs_dom_lim.beg_auth_comp_id / _struct_ncs_dom_lim.beg_label_asym_id / _struct_ncs_dom_lim.beg_label_comp_id / _struct_ncs_dom_lim.beg_label_seq_id / _struct_ncs_dom_lim.end_auth_comp_id / _struct_ncs_dom_lim.end_label_asym_id / _struct_ncs_dom_lim.end_label_comp_id / _struct_ncs_dom_lim.end_label_seq_id / _struct_site.pdbx_auth_asym_id / _struct_site.pdbx_auth_comp_id / _struct_site.pdbx_auth_seq_id

Structure viewer	Molecule: MolmilJmol/JSmol

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PDBx/mmCIF format	4hsn.cif.gz	534.3 KB	Display	PDBx/mmCIF format
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Validation report

Arichive directory	https://data.pdbj.org/pub/pdb/validation_reports/hs/4hsn ftp://data.pdbj.org/pub/pdb/validation_reports/hs/4hsn	HTTPS FTP

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Related structure data

Related structure data	4hsoC 4ixxC 1of8S C: citing same article (ref.) S: Starting model for refinement
Similar structure data	4ixx-d 5dce-d 5d05-d 5dcb-d 1qr7-d 4ucg-d 4uma-d 5cz0-d 1n8f-d 5czt-d Search similar-shape structures by Omokage search (details) Similarity search - Function & homologyF&H Search Similarity search - FunctionF&H Search Similarity search - HomologyF&H Search

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Links

PDB pages	PDBj / wwPDB / NCBI
Related items in Molecule of the Month	#50 - Feb 2004 Glycolytic Enzymes similarity (1)

Deposited unit

A: 3-deoxy-D-arabino-heptulosonate 7-phosphate synthase

B: 3-deoxy-D-arabino-heptulosonate 7-phosphate synthase

C: 3-deoxy-D-arabino-heptulosonate 7-phosphate synthase

D: 3-deoxy-D-arabino-heptulosonate 7-phosphate synthase

hetero molecules

156 kDa, 4 polymers
Search similar-shape structures of this assembly by Omokage search (details)

1

Idetical with deposited unit
defined by author&software

Unit cell

Length a, b, c (Å)	73.462, 137.279, 76.360
Angle α, β, γ (deg.)	90.000, 96.420, 90.000
Int Tables number	4
Space group name H-M	P12₁1

Noncrystallographic symmetry (NCS)

NCS domain:

ID	Ens-ID	Details
1	1	A
2	1	B
1	2	A
2	2	C
1	3	A
2	3	D
1	4	B
2	4	C
1	5	B
2	5	D
1	6	C
2	6	D

NCS domain segments:

Component-ID: 0 / Beg auth comp-ID: ILE / Beg label comp-ID: ILE / End auth comp-ID: ALA / End label comp-ID: ALA / Refine code: 0 / Auth seq-ID: 10 - 350 / Label seq-ID: 10 - 350

Dom-ID	Ens-ID	Auth asym-ID	Label asym-ID
1	1	A	A
2	1	B	B
1	2	A	A
2	2	C	C
1	3	A	A
2	3	D	D
1	4	B	B
2	4	C	C
1	5	B	B
2	5	D	D
1	6	C	C
2	6	D	D

NCS ensembles :

ID
1
2
3
4
5
6

#1: Protein	3-deoxy-D-arabino-heptulosonate 7-phosphate synthase / Phospho-2-dehydro-3-deoxyheptonate aldolase / DAHP synthase / Phospho-2-keto-3-deoxyheptonate aldolase Mass: 38712.133 Da / Num. of mol.: 4 Source method: isolated from a genetically manipulated source Source: (gene. exp.) Neisseria meningitidis (bacteria) / Strain: MC58 (serogroup B) / Gene: aroG, NMB0307 / Plasmid: pT7-7 / Production host: Escherichia coli (E. coli) / Strain (production host): BL21(DE3) References: UniProt: Q9K169, 3-deoxy-7-phosphoheptulonate synthase
#2: Chemical	ChemComp-MN / MANGANESE (II) ION Mass: 54.938 Da / Num. of mol.: 4 / Source method: obtained synthetically / Formula: Mn
#3: Chemical	ChemComp-SO4 / SULFATE ION / Sulfate Mass: 96.063 Da / Num. of mol.: 4 / Source method: obtained synthetically / Formula: SO₄
#4: Chemical	ChemComp-PEP / PHOSPHOENOLPYRUVATE / Phosphoenolpyruvic acid Mass: 168.042 Da / Num. of mol.: 4 / Source method: obtained synthetically / Formula: C₃H₅O₆P
#5: Water	ChemComp-HOH / water / Water Mass: 18.015 Da / Num. of mol.: 561 / Source method: isolated from a natural source / Formula: H₂O

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Experiment

Experiment	Method: X-RAY DIFFRACTION / Number of used crystals: 1

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Sample preparation

Crystal	Density Matthews: 2.47 Å³/Da / Density % sol: 50.22 %
Crystal grow	Temperature: 293.15 K / Method: vapor diffusion, hanging drop / pH: 8.5 Details: A protein solution [11 mg/mL in 10 mM BTP buffer (pH 7.3)] was mixed 1:1 (v/v) with a reservoir solution containing 0.2 M trimethylamine N-oxide, 0.1 M Tris (pH 8.5), 15% 20% (w/v) PEG 2000 ...Details: A protein solution [11 mg/mL in 10 mM BTP buffer (pH 7.3)] was mixed 1:1 (v/v) with a reservoir solution containing 0.2 M trimethylamine N-oxide, 0.1 M Tris (pH 8.5), 15% 20% (w/v) PEG 2000 mme, 0.4 mM MnSO4. The drop sizes were 2 uL, and the volume of the reservoir solution was 500 uL, VAPOR DIFFUSION, HANGING DROP, temperature 293.15K

Crystal

Density Matthews: 2.47 Å³/Da / Density % sol: 50.22 %

Crystal grow

Temperature: 293.15 K / Method: vapor diffusion, hanging drop / pH: 8.5
Details: A protein solution [11 mg/mL in 10 mM BTP buffer (pH 7.3)] was mixed 1:1 (v/v) with a reservoir solution containing 0.2 M trimethylamine N-oxide, 0.1 M Tris (pH 8.5), 15% 20% (w/v) PEG 2000 ...

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Data collection

Diffraction	Mean temperature: 100 K
Diffraction source	Source: SYNCHROTRON / Site: Australian Synchrotron / Beamline: MX2 / Wavelength: 0.9537 Å
Detector	Type: ADSC QUANTUM 315r / Detector: CCD
Radiation	Protocol: SINGLE WAVELENGTH / Monochromatic (M) / Laue (L): M / Scattering type: x-ray
Radiation wavelength	Wavelength: 0.9537 Å / Relative weight: 1
Reflection	Resolution: 2→19.79 Å / Num. all: 100515 / Num. obs: 100515 / % possible obs: 99.3 %
Reflection shell	Resolution: 2→2.03 Å / % possible all: 100

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Phasing

Phasing	Method: molecular replacement

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Processing

Software

Name	Version	Classification
PHASER		phasing
REFMAC		refinement
PDB_EXTRACT	3.11	data extraction
ADSC	Quantum	data collection
XSCALE		data scaling
Aimless		data scaling

Refinement

Method to determine structure:

MOLECULAR REPLACEMENT
Starting model: PDB ENTRY 1OF8

1of8

Resolution: 2→19.79 Å / Cor.coef. F_o:F_c: 0.963 / Cor.coef. F_o:F_c free: 0.946 / WR_factor R_free: 0.214 / WR_factor R_work: 0.1796 / Occupancy max: 1 / Occupancy min: 0.5 / FOM work R set: 0.8641 / SU B: 7.837 / SU ML: 0.109 / SU R Cruickshank DPI: 0.169 / SU R_free: 0.1463 / Cross valid method: THROUGHOUT / σ(F): 0 / ESU R: 0.169 / ESU R Free: 0.146 / Stereochemistry target values: MAXIMUM LIKELIHOOD
Details: HYDROGENS HAVE BEEN ADDED IN THE RIDING POSITIONS U VALUES : WITH TLS ADDED

	R_factor	Num. reflection	% reflection	Selection details
R_free	0.2085	6233	6.2 %	RANDOM
R_work	0.1754	-	-	-
obs	0.1774	100477	99.24 %	-

Solvent computation

Ion probe radii: 0.8 Å / Shrinkage radii: 0.8 Å / VDW probe radii: 1.2 Å / Solvent model: MASK

Displacement parameters

B_iso max: 108.47 Å² / B_iso mean: 37.0606 Å² / B_iso min: 20.43 Å²

	B_aniso -1	B_aniso -2	B_aniso -3
1-	-4.63 Å²	0 Å²	-0.03 Å²
2-	-	4.22 Å²	0 Å²
3-	-	-	0.41 Å²

Refinement step

Cycle: LAST / Resolution: 2→19.79 Å

	Protein	Nucleic acid	Ligand	Solvent	Total
Num. atoms	10432	0	64	561	11057

Refine LS restraints

Refine-ID	Type	Dev ideal	Dev ideal target	Number
X-RAY DIFFRACTION	r_bond_refined_d	0.019	0.019	10683
X-RAY DIFFRACTION	r_bond_other_d	0.01	0.02	10291
X-RAY DIFFRACTION	r_angle_refined_deg	1.781	1.968	14460
X-RAY DIFFRACTION	r_angle_other_deg	1.478	3	23654
X-RAY DIFFRACTION	r_dihedral_angle_1_deg	5.529	5	1362
X-RAY DIFFRACTION	r_dihedral_angle_2_deg	36.391	23.996	463
X-RAY DIFFRACTION	r_dihedral_angle_3_deg	14.428	15	1846
X-RAY DIFFRACTION	r_dihedral_angle_4_deg	20.536	15	73
X-RAY DIFFRACTION	r_chiral_restr	0.113	0.2	1628
X-RAY DIFFRACTION	r_gen_planes_refined	0.012	0.021	12124
X-RAY DIFFRACTION	r_gen_planes_other	0.008	0.02	2365

Refine LS restraints NCS

Refine-ID: X-RAY DIFFRACTION / Type: LOCAL / Weight: 0.05

Ens-ID	Dom-ID	Auth asym-ID	Number	Rms
1	1	A	21081	0.08
1	2	B	21081	0.08
2	1	A	20900	0.09
2	2	C	20900	0.09
3	1	A	21345	0.08
3	2	D	21345	0.08
4	1	B	20776	0.1
4	2	C	20776	0.1
5	1	B	20935	0.09
5	2	D	20935	0.09
6	1	C	20808	0.09
6	2	D	20808	0.09

LS refinement shell

Resolution: 2→2.052 Å / Total num. of bins used: 20

	R_factor	Num. reflection	% reflection
R_free	0.276	494	-
R_work	0.241	6971	-
all	-	7465	-
obs	-	-	99.97 %

Refinement TLS params.

Method: refined / Refine-ID: X-RAY DIFFRACTION

ID	L11 (°²)	L12 (°²)	L13 (°²)	L22 (°²)	L23 (°²)	L33 (°²)	S11 (Å °)	S12 (Å °)	S13 (Å °)	S21 (Å °)	S22 (Å °)	S23 (Å °)	S31 (Å °)	S32 (Å °)	S33 (Å °)	T11 (Å²)	T12 (Å²)	T13 (Å²)	T22 (Å²)	T23 (Å²)	T33 (Å²)	Origin x (Å)	Origin y (Å)	Origin z (Å)
1	2.8307	-0.0382	-0.384	2.1699	-0.4359	1.1207	0.0553	0.185	0.33	-0.0266	-0.0338	-0.1186	-0.1056	-0.0362	-0.0215	0.2715	-0.0166	-0.0381	0.0681	0.0528	0.2311	-3.445	-69.646	-16.549
2	1.2814	-0.0668	0.1088	0.8777	-0.0814	0.555	-0.0348	0.0809	-0.0483	-0.0481	-0.0167	-0.0825	0.0607	0.0108	0.0515	0.2648	-0.01	-0.004	0.0247	0.0101	0.2253	0.783	-82.162	-9.398
3	1.3204	-0.254	-0.002	1.2108	-0.7733	0.864	-0.0241	0.1703	-0.0825	-0.0597	0.0412	0.1085	0.0901	-0.0625	-0.0171	0.2719	-0.0295	-0.0211	0.0326	-0.0321	0.2174	-16.548	-85.374	-11.432
4	3.8416	0.6281	1.4837	2.4928	1.0688	4.1287	0.0415	-0.123	-0.5691	-0.001	-0.0885	0.1387	0.3646	-0.1057	0.047	0.3009	-0.0325	0.0169	0.0123	-0.0161	0.3226	-11.724	-100.935	-2.942
5	2.9347	1.9007	2.2593	8.1562	6.1189	9.8182	-0.0339	-0.227	-0.3002	0.1828	-0.1448	0.1068	0.3794	0.1051	0.1787	0.2892	0.0059	0.0039	0.0351	0.0562	0.3423	0.226	-101.614	2.94
6	1.6713	0.6508	0.1903	2.8159	0.7715	1.46	-0.0306	-0.2752	-0.0554	0.1123	-0.0972	-0.0172	-0.0061	0.0624	0.1278	0.2775	0.0145	-0.0139	0.056	0.0459	0.2318	4.942	-85.872	2.764
7	3.4007	3.0354	1.0031	10.9866	5.1885	7.4614	-0.0199	0.0727	-0.5658	0.0693	-0.1767	-0.1078	0.6089	0.0494	0.1966	0.2959	0.0599	0.0629	0.0228	0.0001	0.378	6.306	-104.375	-7.281
8	2.1844	0.1272	-0.1103	1.156	-1.1394	2.8463	0.0252	0.1735	0.2691	-0.0179	0.1109	0.0575	-0.1601	-0.0485	-0.1361	0.2362	0.0025	-0.0171	0.0323	0.0661	0.3161	-31.03	-62.933	-8.061
9	2.2595	-0.4478	-0.5001	1.5138	0.2595	3.5145	-0.0414	-0.0047	0.1208	0.0206	0.2114	0.1727	0.0717	-0.3556	-0.17	0.2438	-0.0331	-0.033	0.1199	0.1152	0.274	-44.24	-71.208	1.071
10	1.7474	0.0825	-0.3077	0.7051	-0.7481	1.1563	-0.0556	0.1195	0.0394	-0.1234	0.149	0.0476	0.1775	-0.1356	-0.0934	0.2349	-0.0202	-0.0403	0.067	0.0797	0.2133	-33.119	-71.736	-11.301
11	1.7978	0.1948	-0.5333	0.8088	-0.5568	1.0079	-0.0155	-0.1427	0.0311	0.0366	0.0129	-0.0122	0.0567	0.0105	0.0027	0.2562	-0.0059	-0.0356	0.0559	-0.0011	0.2159	-22.192	-73.995	2.618
12	2.7392	0.6184	0.9405	1.3338	0.1096	2.0767	0.0079	-0.3382	-0.2905	0.0273	0.1788	0.0137	0.2342	-0.2293	-0.1867	0.2556	-0.0024	-0.028	0.1147	0.0954	0.1721	-35.997	-83.203	13.082
13	2.0909	-0.4107	0.1991	1.008	0.5416	0.463	-0.0131	-0.0648	-0.2445	-0.0767	0.002	0.0946	-0.0116	-0.117	0.0111	0.2787	-0.0556	-0.0151	0.1418	0.0579	0.2626	-45.59	-81.412	-0.168
14	12.0392	-0.0106	-1.0006	9.0869	4.0533	7.2252	0.0423	-1.0678	-0.0657	0.7153	0.2849	0.0277	-0.3889	-0.5406	-0.3272	0.2227	0.0915	0.0349	0.3029	0.1388	0.1618	-47.012	-72.704	19.686
15	1.7477	0.1618	0.0607	2.9406	-0.6566	0.7685	0.0523	-0.0402	-0.0794	0.0847	0.0036	0.0472	-0.0324	-0.034	-0.0559	0.3059	-0.0057	0.0026	0.1023	0.071	0.2302	0.448	-45.433	-21.372
16	1.3511	0.898	0.1901	4.4786	0.2615	2.2115	-0.0776	0.0825	-0.0005	-0.019	0.0144	-0.2958	0.0288	0.1422	0.0632	0.2245	-0.0257	-0.0039	0.0637	0.1072	0.2315	14.457	-37.491	-27.919
17	1.5768	0.347	-0.3269	1.2443	-0.2244	0.2162	-0.0405	0.2815	-0.021	-0.2035	0.0605	-0.0001	0.0512	-0.0646	-0.02	0.2527	-0.0175	-0.0262	0.0822	0.075	0.1953	2.97	-48.933	-30.456
18	1.7556	0.7847	-0.1503	1.3069	-0.7653	0.9048	-0.0248	0.2041	0.2203	0.0303	0.0826	0.1057	-0.1108	0.0003	-0.0578	0.2328	-0.0056	-0.0116	0.0532	0.1049	0.2541	-7.909	-34.232	-30.913
19	2.6508	-0.4813	-0.6494	1.2239	-0.1369	1.6742	-0.1074	0.361	0.3584	-0.1063	-0.0576	0.1337	-0.1397	0.1668	0.165	0.2942	-0.0665	-0.0673	0.1248	0.1713	0.3084	5.611	-23.129	-37.718
20	1.485	0.2311	-0.1845	2.7094	0.5066	2.2396	-0.2084	0.3431	0.0817	-0.2803	0.0281	-0.2168	0.0958	0.1855	0.1803	0.2596	-0.069	0.0299	0.1547	0.1333	0.2289	15.465	-34.608	-36.987
21	17.9931	-0.9846	-0.2672	11.0923	-5.5376	16.3442	-0.1702	-0.1843	0.8283	0.7224	0.431	0.104	-0.9027	-0.3186	-0.2608	0.3238	-0.0578	-0.0825	0.0588	0.07	0.2818	15.127	-15.698	-24.13
22	1.9235	-0.0865	0.3187	1.1339	-0.7521	2.4191	0.1402	-0.0858	-0.1414	-0.0962	0.0854	0.1132	0.1319	-0.0421	-0.2256	0.2941	0.0019	-0.0642	0.0716	0.121	0.3196	-26.562	-53.807	-37.368
23	1.0542	-0.2888	0.4033	0.7724	-0.2435	1.3559	0.0204	0.0193	-0.0124	-0.0756	0.0577	0.1587	0.0507	-0.0271	-0.0781	0.2127	-0.0214	-0.048	0.0781	0.1541	0.308	-32.288	-43.896	-38.977
24	0.8342	-0.1815	0.594	0.6973	-0.3781	0.9192	0.0703	0.2283	-0.0176	-0.1151	-0.0613	0.0291	0.0897	0.2387	-0.0089	0.2216	0.0103	-0.0479	0.1354	0.0983	0.1759	-15.347	-46.546	-44.325
25	2.3365	-1.2019	0.582	1.9396	0.022	1.7505	0.0046	0.339	0.1438	-0.2678	0.0449	0.167	-0.1199	0.3056	-0.0495	0.2836	-0.0552	-0.0658	0.1759	0.1481	0.2148	-22.981	-34.015	-57.669
26	2.9139	-1.2179	-2.9327	2.121	1.6201	5.3564	-0.01	0.1823	0.0802	-0.1526	0.0607	0.3542	-0.0849	0.1779	-0.0507	0.2439	-0.0319	-0.1151	0.1264	0.1448	0.3489	-31.92	-34.435	-50.338
27	2.7466	0.8041	-0.0241	0.9336	0.6978	2.2445	-0.0564	-0.1634	0.26	0.0362	0.1534	0.4202	-0.1183	-0.132	-0.097	0.2574	0.0367	-0.044	0.1411	0.1741	0.4439	-38.306	-31.561	-39.353
28	6.1995	-1.8877	-3.8671	6.4346	4.4791	12.9736	0.1807	0.4942	-0.1368	-0.5364	0.033	0.0921	-0.0382	0.0637	-0.2137	0.2745	-0.0159	-0.1404	0.1466	0.1284	0.2796	-36.419	-39.491	-62.81

Refinement TLS group

ID	Refine-ID	Refine TLS-ID	Auth asym-ID	Auth seq-ID
1	X-RAY DIFFRACTION	1	A	10 - 40
2	X-RAY DIFFRACTION	2	A	41 - 163
3	X-RAY DIFFRACTION	3	A	164 - 239
4	X-RAY DIFFRACTION	4	A	240 - 273
5	X-RAY DIFFRACTION	5	A	274 - 295
6	X-RAY DIFFRACTION	6	A	296 - 331
7	X-RAY DIFFRACTION	7	A	332 - 350
8	X-RAY DIFFRACTION	8	B	10 - 50
9	X-RAY DIFFRACTION	9	B	51 - 98
10	X-RAY DIFFRACTION	10	B	99 - 164
11	X-RAY DIFFRACTION	11	B	165 - 243
12	X-RAY DIFFRACTION	12	B	244 - 292
13	X-RAY DIFFRACTION	13	B	293 - 338
14	X-RAY DIFFRACTION	14	B	339 - 350
15	X-RAY DIFFRACTION	15	C	10 - 53
16	X-RAY DIFFRACTION	16	C	54 - 98
17	X-RAY DIFFRACTION	17	C	99 - 162
18	X-RAY DIFFRACTION	18	C	163 - 245
19	X-RAY DIFFRACTION	19	C	246 - 291
20	X-RAY DIFFRACTION	20	C	292 - 342
21	X-RAY DIFFRACTION	21	C	343 - 350
22	X-RAY DIFFRACTION	22	D	10 - 53
23	X-RAY DIFFRACTION	23	D	54 - 154
24	X-RAY DIFFRACTION	24	D	155 - 242
25	X-RAY DIFFRACTION	25	D	243 - 291
26	X-RAY DIFFRACTION	26	D	292 - 314
27	X-RAY DIFFRACTION	27	D	315 - 331
28	X-RAY DIFFRACTION	28	D	332 - 350

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