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Yorodumi- PDB-4h7z: tRNA-guanine transglycosylase Y106F, C158V mutant in complex with... -
+Open data
-Basic information
Entry | Database: PDB / ID: 4h7z | ||||||
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Title | tRNA-guanine transglycosylase Y106F, C158V mutant in complex with guanine | ||||||
Components | Queuine tRNA-ribosyltransferase | ||||||
Keywords | TRANSFERASE / substrate specificity / bacterial TGT / queuine / preQ1 / tRNA / guanine exchange enzyme | ||||||
Function / homology | Function and homology information tRNA-guanosine34 preQ1 transglycosylase / tRNA wobble guanine modification / tRNA-guanosine(34) queuine transglycosylase activity / tRNA-guanine transglycosylation / queuosine biosynthetic process / metal ion binding / cytosol Similarity search - Function | ||||||
Biological species | Zymomonas mobilis (bacteria) | ||||||
Method | X-RAY DIFFRACTION / SYNCHROTRON / MOLECULAR REPLACEMENT / Resolution: 1.68 Å | ||||||
Authors | Biela, I. / Tidten-Luksch, N. / Heine, A. / Reuter, K. / Klebe, G. | ||||||
Citation | Journal: Plos One / Year: 2013 Title: Investigation of Specificity Determinants in Bacterial tRNA-Guanine Transglycosylase Reveals Queuine, the Substrate of Its Eucaryotic Counterpart, as Inhibitor. Authors: Biela, I. / Tidten-Luksch, N. / Immekus, F. / Glinca, S. / Nguyen, T.X. / Gerber, H.D. / Heine, A. / Klebe, G. / Reuter, K. | ||||||
History |
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-Structure visualization
Structure viewer | Molecule: MolmilJmol/JSmol |
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-Downloads & links
-Download
PDBx/mmCIF format | 4h7z.cif.gz | 161.8 KB | Display | PDBx/mmCIF format |
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PDB format | pdb4h7z.ent.gz | 124.9 KB | Display | PDB format |
PDBx/mmJSON format | 4h7z.json.gz | Tree view | PDBx/mmJSON format | |
Others | Other downloads |
-Validation report
Arichive directory | https://data.pdbj.org/pub/pdb/validation_reports/h7/4h7z ftp://data.pdbj.org/pub/pdb/validation_reports/h7/4h7z | HTTPS FTP |
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-Related structure data
Related structure data | 2nqzC 2nsoC 3bl3C 3bldC 3bllC 3bloC 4e2vC 4gcxC 4gd0C 4h6eC 4hqvC 4hshC 4hvxC 3gev C: citing same article (ref.) S: Starting model for refinement |
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Similar structure data |
-Links
-Assembly
Deposited unit |
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1 |
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Unit cell |
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-Components
#1: Protein | Mass: 42905.691 Da / Num. of mol.: 1 / Mutation: Y106F, C158V Source method: isolated from a genetically manipulated source Source: (gene. exp.) Zymomonas mobilis (bacteria) / Strain: ATCC 31821 / ZM4 / CP4 / Gene: tgt, ZMO0363 / Plasmid: pET9d-ZM-Y106F_C158V / Production host: Escherichia coli (E. coli) / Strain (production host): BL21 DE(3) pLysS References: UniProt: P28720, tRNA-guanosine34 preQ1 transglycosylase | ||||
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#2: Chemical | ChemComp-GUN / | ||||
#3: Chemical | ChemComp-ZN / | ||||
#4: Chemical | #5: Water | ChemComp-HOH / | Sequence details | THR TO LYS CONFLICT AT 312 IN UNP ENTRY P28720 | |
-Experimental details
-Experiment
Experiment | Method: X-RAY DIFFRACTION / Number of used crystals: 1 |
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-Sample preparation
Crystal | Density Matthews: 2.4 Å3/Da / Density % sol: 48.78 % |
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Crystal grow | Temperature: 291.15 K / Method: vapor diffusion, sitting drop / pH: 8.5 Details: 100 mM Tris, 1 mM DTT, 10% DMSO, 12% PEG 8000 , pH 8.5, VAPOR DIFFUSION, SITTING DROP, temperature 291.15K |
-Data collection
Diffraction | Mean temperature: 113.15 K |
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Diffraction source | Source: SYNCHROTRON / Site: BESSY / Beamline: 14.1 / Wavelength: 0.91841 Å |
Detector | Type: MARMOSAIC 225 mm CCD / Detector: CCD / Date: Sep 15, 2012 / Details: mirror |
Radiation | Monochromator: double crystal monochromator / Protocol: SINGLE WAVELENGTH / Monochromatic (M) / Laue (L): M / Scattering type: x-ray |
Radiation wavelength | Wavelength: 0.91841 Å / Relative weight: 1 |
Reflection | Resolution: 1.68→30 Å / Num. all: 45941 / Num. obs: 45941 / % possible obs: 99.2 % / Redundancy: 2.9 % / Biso Wilson estimate: 17.7 Å2 / Rmerge(I) obs: 0.07 / Net I/σ(I): 10.2 |
Reflection shell | Resolution: 1.68→1.77 Å / Redundancy: 2.8 % / Rmerge(I) obs: 0.0482 / Mean I/σ(I) obs: 2.1 / Num. unique all: 6709 / % possible all: 99.8 |
-Processing
Software |
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Refinement | Method to determine structure: MOLECULAR REPLACEMENT Starting model: PDB entry 3GEV 3gev Resolution: 1.68→29.415 Å / SU ML: 0.17 / Isotropic thermal model: isotropic / Cross valid method: R-free / σ(F): 1.24 / Phase error: 17.88 / Stereochemistry target values: ML
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Solvent computation | Shrinkage radii: 0.9 Å / VDW probe radii: 1.11 Å / Solvent model: FLAT BULK SOLVENT MODEL | |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Refinement step | Cycle: LAST / Resolution: 1.68→29.415 Å
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Refine LS restraints |
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LS refinement shell |
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Refinement TLS params. | Method: refined / Refine-ID: X-RAY DIFFRACTION
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Refinement TLS group |
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