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Yorodumi- PDB-4gzs: N2 neuraminidase D151G mutant of a/Tanzania/205/2010 H3N2 in comp... -
+Open data
-Basic information
Entry | Database: PDB / ID: 4gzs | |||||||||
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Title | N2 neuraminidase D151G mutant of a/Tanzania/205/2010 H3N2 in complex with hepes | |||||||||
Components | Neuraminidase | |||||||||
Keywords | VIRAL PROTEIN / beta-propella / influenza virus / neuraminidase / hemagglutinin / hemadsorption / viral infection | |||||||||
Function / homology | Function and homology information exo-alpha-(2->3)-sialidase activity / exo-alpha-(2->6)-sialidase activity / exo-alpha-(2->8)-sialidase activity / exo-alpha-sialidase / viral budding from plasma membrane / membrane => GO:0016020 / carbohydrate metabolic process / host cell plasma membrane / virion membrane / metal ion binding Similarity search - Function | |||||||||
Biological species | Influenza A virus | |||||||||
Method | X-RAY DIFFRACTION / SYNCHROTRON / MOLECULAR REPLACEMENT / Resolution: 2.35 Å | |||||||||
Authors | Zhu, X. / Wilson, I.A. | |||||||||
Citation | Journal: J.Virol. / Year: 2012 Title: Influenza virus neuraminidases with reduced enzymatic activity that avidly bind sialic Acid receptors. Authors: Zhu, X. / McBride, R. / Nycholat, C.M. / Yu, W. / Paulson, J.C. / Wilson, I.A. | |||||||||
History |
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-Structure visualization
Structure viewer | Molecule: MolmilJmol/JSmol |
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-Downloads & links
-Download
PDBx/mmCIF format | 4gzs.cif.gz | 321.4 KB | Display | PDBx/mmCIF format |
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PDB format | pdb4gzs.ent.gz | 262.4 KB | Display | PDB format |
PDBx/mmJSON format | 4gzs.json.gz | Tree view | PDBx/mmJSON format | |
Others | Other downloads |
-Validation report
Arichive directory | https://data.pdbj.org/pub/pdb/validation_reports/gz/4gzs ftp://data.pdbj.org/pub/pdb/validation_reports/gz/4gzs | HTTPS FTP |
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-Related structure data
Related structure data | 4gzoSC 4gzpC 4gzqC 4gztC 4gzwC 4gzxC S: Starting model for refinement C: citing same article (ref.) |
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Similar structure data |
-Links
-Assembly
Deposited unit |
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1 |
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Unit cell |
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-Components
-Protein , 1 types, 4 molecules ABCD
#1: Protein | Mass: 43291.316 Da / Num. of mol.: 4 / Fragment: ectodomain Source method: isolated from a genetically manipulated source Source: (gene. exp.) Influenza A virus / Strain: A/Tanzania/205/2010(H3N2) / Plasmid: PFASTBACHT-A / Production host: Trichoplusia Ni (cabbage looper) / References: UniProt: K7N5N8*PLUS |
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-Sugars , 4 types, 15 molecules
#2: Polysaccharide | alpha-D-mannopyranose-(1-3)-[alpha-D-mannopyranose-(1-6)]beta-D-mannopyranose-(1-4)-2-acetamido-2- ...alpha-D-mannopyranose-(1-3)-[alpha-D-mannopyranose-(1-6)]beta-D-mannopyranose-(1-4)-2-acetamido-2-deoxy-beta-D-glucopyranose-(1-4)-2-acetamido-2-deoxy-beta-D-glucopyranose Source method: isolated from a genetically manipulated source #3: Polysaccharide | Source method: isolated from a genetically manipulated source #4: Polysaccharide | 2-acetamido-2-deoxy-beta-D-glucopyranose-(1-4)-2-acetamido-2-deoxy-beta-D-glucopyranose | Source method: isolated from a genetically manipulated source #7: Sugar | ChemComp-NAG / |
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-Non-polymers , 3 types, 372 molecules
#5: Chemical | ChemComp-CA / #6: Chemical | ChemComp-EPE / #8: Water | ChemComp-HOH / | |
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-Details
Sequence details | AUTHORS STATE THAT THE GISAID ACCESSION NUMBER IS EPI279969 FOR THIS SEQUENCE. |
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-Experimental details
-Experiment
Experiment | Method: X-RAY DIFFRACTION / Number of used crystals: 1 |
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-Sample preparation
Crystal | Density Matthews: 2.82 Å3/Da / Density % sol: 56.38 % |
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Crystal grow | Temperature: 295 K / Method: vapor diffusion / pH: 7.5 Details: 0.1 M hepes, 17-25% PEG 8000, pH 7.5, VAPOR DIFFUSION, temperature 295K |
-Data collection
Diffraction | Mean temperature: 100 K |
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Diffraction source | Source: SYNCHROTRON / Site: APS / Beamline: 23-ID-B / Wavelength: 1.0332 Å |
Detector | Type: MARMOSAIC 300 mm CCD / Detector: CCD / Date: Jun 16, 2011 |
Radiation | Monochromator: Double crystal cryo-cooled Si(111) / Protocol: SINGLE WAVELENGTH / Monochromatic (M) / Laue (L): M / Scattering type: x-ray |
Radiation wavelength | Wavelength: 1.0332 Å / Relative weight: 1 |
Reflection | Resolution: 2.35→50 Å / Num. obs: 74532 / % possible obs: 92.1 % / Observed criterion σ(I): -3 / Redundancy: 3.3 % / Biso Wilson estimate: 40.9 Å2 / Rmerge(I) obs: 0.118 / Net I/σ(I): 13.2 |
Reflection shell | Resolution: 2.35→2.43 Å / Redundancy: 2.3 % / Rmerge(I) obs: 0.473 / Mean I/σ(I) obs: 1.9 / % possible all: 63.2 |
-Processing
Software |
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Refinement | Method to determine structure: MOLECULAR REPLACEMENT Starting model: PDB entry 4GZO Resolution: 2.35→50 Å / Cor.coef. Fo:Fc: 0.926 / Cor.coef. Fo:Fc free: 0.889 / SU B: 10.322 / SU ML: 0.239 / Cross valid method: THROUGHOUT / ESU R: 0.468 / ESU R Free: 0.304 / Stereochemistry target values: MAXIMUM LIKELIHOOD / Details: HYDROGENS HAVE BEEN ADDED IN THE RIDING POSITIONS
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Solvent computation | Ion probe radii: 0.8 Å / Shrinkage radii: 0.8 Å / VDW probe radii: 1.4 Å / Solvent model: MASK | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Displacement parameters | Biso mean: 29.912 Å2
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Refinement step | Cycle: LAST / Resolution: 2.35→50 Å
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Refine LS restraints |
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LS refinement shell | Resolution: 2.35→2.411 Å / Total num. of bins used: 20
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