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Open data
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Basic information
Entry | Database: PDB / ID: 4fzf | ||||||
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Title | Crystal structure of MST4-MO25 complex with DKI | ||||||
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Function / homology | ![]() negative regulation of potassium ion transmembrane transporter activity / ![]() ![]() ![]() ![]() ![]() ![]() ![]() ![]() ![]() ![]() ![]() ![]() ![]() ![]() Similarity search - Function | ||||||
Biological species | ![]() ![]() | ||||||
Method | ![]() ![]() ![]() | ||||||
![]() | Shi, Z.B. / Zhou, Z.C. | ||||||
![]() | ![]() Title: Structure of the MST4 in Complex with MO25 Provides Insights into Its Activation Mechanism Authors: Shi, Z. / Jiao, S. / Zhang, Z. / Ma, M. / Zhang, Z. / Chen, C. / Wang, K. / Wang, H. / Wang, W. / Zhang, L. / Zhao, Y. / Zhou, Z. | ||||||
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Structure visualization
Structure viewer | Molecule: ![]() ![]() |
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Downloads & links
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Download
PDBx/mmCIF format | ![]() | 263.3 KB | Display | ![]() |
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PDB format | ![]() | 217 KB | Display | ![]() |
PDBx/mmJSON format | ![]() | Tree view | ![]() | |
Others | ![]() |
-Validation report
Arichive directory | ![]() ![]() | HTTPS FTP |
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-Related structure data
Related structure data | ![]() 4fzaC ![]() 4fzdC ![]() 1uplS ![]() 3ggfS C: citing same article ( S: Starting model for refinement |
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Similar structure data |
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Links
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Assembly
Deposited unit | ![]()
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Unit cell |
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Components
#1: Protein | Mass: 38311.098 Da / Num. of mol.: 1 / Fragment: Mo25-like, UNP residues 11-334 Source method: isolated from a genetically manipulated source Source: (gene. exp.) ![]() ![]() ![]() ![]() ![]() |
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#2: Protein | Mass: 31930.670 Da / Num. of mol.: 1 / Fragment: Kinase domain, UNP residues 18-297 / Mutation: D162A Source method: isolated from a genetically manipulated source Source: (gene. exp.) ![]() ![]() ![]() ![]() ![]() References: UniProt: Q9P289, ![]() |
#3: Chemical | ChemComp-DKI / |
-Experimental details
-Experiment
Experiment | Method: ![]() |
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Sample preparation
Crystal | Density Matthews: 4.07 Å3/Da / Density % sol: 69.8 % |
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Crystal grow![]() | Temperature: 289 K / Method: vapor diffusion, hanging drop / pH: 8 Details: 0.1M Tris pH 8.0, 20% PEG 350 mme, VAPOR DIFFUSION, HANGING DROP, temperature 289K |
-Data collection
Diffraction | Mean temperature: 100 K |
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Diffraction source | Source: ![]() ![]() ![]() |
Detector | Type: ADSC QUANTUM 315 / Detector: CCD / Date: Sep 10, 2011 |
Radiation | Monochromator: Si 111 CHANNEL / Protocol: SINGLE WAVELENGTH / Monochromatic (M) / Laue (L): M / Scattering type: x-ray |
Radiation wavelength | Wavelength![]() |
Reflection | Resolution: 3.64→50 Å / Num. all: 13410 / Num. obs: 13356 / % possible obs: 99.6 % / Observed criterion σ(F): 2 / Observed criterion σ(I): 2 / Redundancy: 12.8 % |
Reflection shell | Resolution: 3.64→3.71 Å / Redundancy: 13.6 % / Mean I/σ(I) obs: 4.1 / Num. unique all: 651 / % possible all: 100 |
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Processing
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Refinement | Method to determine structure![]() ![]() Starting model: PDB ENTRY: 1UPL and 3GGF Resolution: 3.64→38.85 Å / Cor.coef. Fo:Fc: 0.911 / Cor.coef. Fo:Fc free: 0.831 / SU B: 73.766 / SU ML: 0.499 / Cross valid method: THROUGHOUT / σ(F): 2 / ESU R Free: 0.715 / Stereochemistry target values: MAXIMUM LIKELIHOOD / Details: HYDROGENS HAVE BEEN USED IF PRESENT IN THE INPUT
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Solvent computation | Ion probe radii: 0.8 Å / Shrinkage radii: 0.8 Å / VDW probe radii: 1.2 Å / Solvent model: MASK | |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Displacement parameters | Biso mean: 116.313 Å2 | |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Refinement step | Cycle: LAST / Resolution: 3.64→38.85 Å
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Refine LS restraints |
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LS refinement shell | Resolution: 3.644→3.739 Å / Total num. of bins used: 20
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Refinement TLS params. | Method: refined / Refine-ID: X-RAY DIFFRACTION
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Refinement TLS group |
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