+Open data
-Basic information
Entry | Database: PDB / ID: 1upl | ||||||
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Title | Crystal structure of MO25 alpha | ||||||
Components | MO25 PROTEINCAB39 | ||||||
Keywords | TRANSFERASE / STRAD / LKB1 / ARMADILLO | ||||||
Function / homology | Function and homology information negative regulation of potassium ion transmembrane transporter activity / negative regulation of potassium ion transmembrane transport / serine/threonine protein kinase complex / cellular hypotonic response / response to thyroid hormone / Energy dependent regulation of mTOR by LKB1-AMPK / protein kinase activator activity / protein serine/threonine kinase activator activity / response to activity / positive regulation of peptidyl-threonine phosphorylation ...negative regulation of potassium ion transmembrane transporter activity / negative regulation of potassium ion transmembrane transport / serine/threonine protein kinase complex / cellular hypotonic response / response to thyroid hormone / Energy dependent regulation of mTOR by LKB1-AMPK / protein kinase activator activity / protein serine/threonine kinase activator activity / response to activity / positive regulation of peptidyl-threonine phosphorylation / positive regulation of protein serine/threonine kinase activity / Z disc / kinase binding / peptidyl-serine phosphorylation / secretory granule lumen / ficolin-1-rich granule lumen / intracellular signal transduction / Neutrophil degranulation / signal transduction / extracellular exosome / extracellular region / cytosol / cytoplasm Similarity search - Function | ||||||
Biological species | HOMO SAPIENS (human) | ||||||
Method | X-RAY DIFFRACTION / SYNCHROTRON / MAD / Resolution: 2.6 Å | ||||||
Authors | Milburn, C.C. / Boudeau, J. / Deak, M. / Alessi, D.R. / Van Aalten, D.M.F. | ||||||
Citation | Journal: Nat.Struct.Mol.Biol. / Year: 2004 Title: Crystal Structure of Mo25 Alpha in Complex with the C-Terminus of the Pseudo Kinase Ste-20 Related Adaptor (Strad) Authors: Milburn, C.C. / Boudeau, J. / Deak, M. / Alessi, D.R. / Van Aalten, D.M.F. | ||||||
History |
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-Structure visualization
Structure viewer | Molecule: MolmilJmol/JSmol |
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-Downloads & links
-Download
PDBx/mmCIF format | 1upl.cif.gz | 138.3 KB | Display | PDBx/mmCIF format |
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PDB format | pdb1upl.ent.gz | 109.7 KB | Display | PDB format |
PDBx/mmJSON format | 1upl.json.gz | Tree view | PDBx/mmJSON format | |
Others | Other downloads |
-Validation report
Arichive directory | https://data.pdbj.org/pub/pdb/validation_reports/up/1upl ftp://data.pdbj.org/pub/pdb/validation_reports/up/1upl | HTTPS FTP |
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-Related structure data
Related structure data | 1upkSC S: Starting model for refinement C: citing same article (ref.) |
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Similar structure data |
-Links
-Assembly
Deposited unit |
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1 |
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2 |
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Unit cell |
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-Components
#1: Protein | Mass: 40344.984 Da / Num. of mol.: 2 Source method: isolated from a genetically manipulated source Source: (gene. exp.) HOMO SAPIENS (human) / Plasmid: PGEX6P-1 / Production host: ESCHERICHIA COLI (E. coli) / Variant (production host): B834 / References: UniProt: Q9Y376 #2: Water | ChemComp-HOH / | |
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-Experimental details
-Experiment
Experiment | Method: X-RAY DIFFRACTION / Number of used crystals: 1 |
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-Sample preparation
Crystal | Density Matthews: 2 Å3/Da / Density % sol: 38 % | ||||||||||||||||||||||||||||||
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Crystal grow | pH: 6.5 Details: 15 % PEG 20000, 0.1 M MES PH 6.5, 4.4 % GAMMA BUTYROLACTONE | ||||||||||||||||||||||||||||||
Crystal grow | *PLUS pH: 6.5 / Method: vapor diffusion, hanging drop | ||||||||||||||||||||||||||||||
Components of the solutions | *PLUS
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-Data collection
Diffraction | Mean temperature: 100 K |
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Diffraction source | Source: SYNCHROTRON / Site: ESRF / Beamline: ID14-4 / Wavelength: 0.933 |
Detector | Type: ADSC Q4 CCD / Detector: CCD / Date: Feb 15, 2003 |
Radiation | Protocol: SINGLE WAVELENGTH / Monochromatic (M) / Laue (L): M / Scattering type: x-ray |
Radiation wavelength | Wavelength: 0.933 Å / Relative weight: 1 |
Reflection | Resolution: 2.6→30 Å / Num. obs: 20073 / % possible obs: 98.6 % / Redundancy: 5.3 % / Rmerge(I) obs: 0.118 / Net I/σ(I): 16.3 |
Reflection shell | Resolution: 2.6→2.69 Å / Redundancy: 4.3 % / Rmerge(I) obs: 0.522 / Mean I/σ(I) obs: 2.7 / % possible all: 90.6 |
Reflection | *PLUS Highest resolution: 2.6 Å / Lowest resolution: 30 Å / Redundancy: 5.3 % / Num. measured all: 106613 / Rmerge(I) obs: 0.118 |
Reflection shell | *PLUS % possible obs: 90.6 % / Redundancy: 4.3 % / Rmerge(I) obs: 0.522 / Mean I/σ(I) obs: 2.7 |
-Processing
Software |
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Refinement | Method to determine structure: MAD Starting model: PDB ENTRY 1UPK Resolution: 2.6→25 Å / Cor.coef. Fo:Fc: 0.928 / Cor.coef. Fo:Fc free: 0.895 / SU B: 13.8 / SU ML: 0.301 / TLS residual ADP flag: LIKELY RESIDUAL / Cross valid method: THROUGHOUT / ESU R Free: 0.374 / Stereochemistry target values: MAXIMUM LIKELIHOOD
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Solvent computation | Ion probe radii: 0.8 Å / Shrinkage radii: 0.8 Å / VDW probe radii: 1.4 Å / Solvent model: BABINET MODEL WITH MASK | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Displacement parameters | Biso mean: 18.9 Å2
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Refinement step | Cycle: LAST / Resolution: 2.6→25 Å
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Refine LS restraints |
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