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- PDB-4fok: 1.8 A Crystal structure of the FimX EAL domain in complex with c-diGMP -
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Open data
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Basic information
Entry | Database: PDB / ID: 4fok | ||||||
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Title | 1.8 A Crystal structure of the FimX EAL domain in complex with c-diGMP | ||||||
![]() | FimX | ||||||
![]() | ![]() ![]() ![]() | ||||||
Function / homology | ![]() | ||||||
Biological species | ![]() ![]() | ||||||
Method | ![]() ![]() ![]() | ||||||
![]() | Farah, C.S. / Guzzo, C.R. | ||||||
![]() | ![]() Title: Structure of the PilZ-FimXEAL-c-di-GMP Complex Responsible for the Regulation of Bacterial Type IV Pilus Biogenesis. Authors: Guzzo, C.R. / Dunger, G. / Salinas, R.K. / Farah, C.S. | ||||||
History |
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Structure visualization
Structure viewer | Molecule: ![]() ![]() |
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Downloads & links
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Download
PDBx/mmCIF format | ![]() | 77.3 KB | Display | ![]() |
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PDB format | ![]() | 56.9 KB | Display | ![]() |
PDBx/mmJSON format | ![]() | Tree view | ![]() | |
Others | ![]() |
-Validation report
Arichive directory | ![]() ![]() | HTTPS FTP |
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-Related structure data
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Links
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Assembly
Deposited unit | ![]()
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Unit cell |
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Components
#1: Protein | Mass: 29612.451 Da / Num. of mol.: 1 / Fragment: EAL domain (UNP residues 426-689) Source method: isolated from a genetically manipulated source Source: (gene. exp.) ![]() ![]() Strain: 306 / Gene: XAC2398 / Plasmid: pET3a / Production host: ![]() ![]() ![]() | ||
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#2: Chemical | ChemComp-C2E / ![]() | ||
#3: Chemical | ChemComp-GOL / ![]() #4: Water | ChemComp-HOH / | ![]() |
-Experimental details
-Experiment
Experiment | Method: ![]() |
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Sample preparation
Crystal | Density Matthews: 2.2 Å3/Da / Density % sol: 44 % |
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Crystal grow![]() | Temperature: 291 K / Method: vapor diffusion, sitting drop / pH: 4.7 Details: 0.6 M amonium sulfate, 0.1 M sodium citrate, pH 4.7, VAPOR DIFFUSION, SITTING DROP, temperature 291K |
-Data collection
Diffraction | Mean temperature: 100 K |
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Diffraction source | Source: ![]() ![]() ![]() |
Detector | Type: MAR CCD 165 mm / Detector: CCD / Date: Mar 30, 2011 |
Radiation | Monochromator: Si(111) / Protocol: SINGLE WAVELENGTH / Monochromatic (M) / Laue (L): M / Scattering type: x-ray |
Radiation wavelength | Wavelength![]() |
Reflection | Resolution: 1.8→30 Å / Num. all: 24851 / Num. obs: 24851 / % possible obs: 99.8 % / Observed criterion σ(F): 0 / Observed criterion σ(I): 0 / Redundancy: 7.4 % / Rsym value: 0.125 / Net I/σ(I): 16.56 |
Reflection shell | Resolution: 1.8→1.86 Å / Redundancy: 7.3 % / Mean I/σ(I) obs: 3.25 / Rsym value: 0.67 / % possible all: 99.4 |
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Processing
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Refinement | Method to determine structure![]() ![]()
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Solvent computation | Ion probe radii: 0.8 Å / Shrinkage radii: 0.8 Å / VDW probe radii: 1.4 Å / Solvent model: BABINET MODEL WITH MASK | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Displacement parameters | Biso mean: 15.536 Å2
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Refinement step | Cycle: LAST / Resolution: 1.8→28.34 Å
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Refine LS restraints |
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LS refinement shell | Resolution: 1.8→1.847 Å / Total num. of bins used: 20
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