+Open data
-Basic information
Entry | Database: PDB / ID: 4fhq | ||||||
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Title | Crystal Structure of HVEM | ||||||
Components | Tumor necrosis factor receptor superfamily member 14 | ||||||
Keywords | IMMUNE SYSTEM / CYSTEINE RICH DOMAIN / TNF RECEPTOR / Structural Genomics / PSI-Biology / Protein Structure Initiative / Atoms-to-Animals: The Immune Function Network / IFN / TNFRSF / Cysteine Rich Domains / Receptor / TNF14 / BTLA / CD160 / gD of HSV / Membrane / New York Structural Genomics Research Consortium (NYSGRC) | ||||||
Function / homology | Function and homology information negative regulation of adaptive immune memory response / negative regulation of alpha-beta T cell proliferation / tumor necrosis factor receptor activity / positive regulation of cytokine production involved in immune response / TNFs bind their physiological receptors / Costimulation by the CD28 family / cytokine binding / positive regulation of T cell migration / T cell costimulation / positive regulation of peptidyl-tyrosine phosphorylation ...negative regulation of adaptive immune memory response / negative regulation of alpha-beta T cell proliferation / tumor necrosis factor receptor activity / positive regulation of cytokine production involved in immune response / TNFs bind their physiological receptors / Costimulation by the CD28 family / cytokine binding / positive regulation of T cell migration / T cell costimulation / positive regulation of peptidyl-tyrosine phosphorylation / virus receptor activity / adaptive immune response / defense response to Gram-negative bacterium / cell surface receptor signaling pathway / defense response to Gram-positive bacterium / immune response / external side of plasma membrane / innate immune response / ubiquitin protein ligase binding / plasma membrane Similarity search - Function | ||||||
Biological species | Homo sapiens (human) | ||||||
Method | X-RAY DIFFRACTION / SYNCHROTRON / MOLECULAR REPLACEMENT / Resolution: 2.251 Å | ||||||
Authors | Liu, W. / Zhan, C. / Patskovsky, Y. / Bhosle, R.C. / Nathenson, S.G. / Almo, S.C. / Atoms-to-Animals: The Immune Function Network (IFN) / New York Structural Genomics Research Consortium (NYSGRC) | ||||||
Citation | Journal: Mol Biotechnol / Year: 2015 Title: Increased Heterologous Protein Expression in Drosophila S2 Cells for Massive Production of Immune Ligands/Receptors and Structural Analysis of Human HVEM. Authors: Liu, W. / Vigdorovich, V. / Zhan, C. / Patskovsky, Y. / Bonanno, J.B. / Nathenson, S.G. / Almo, S.C. | ||||||
History |
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-Structure visualization
Structure viewer | Molecule: MolmilJmol/JSmol |
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-Downloads & links
-Download
PDBx/mmCIF format | 4fhq.cif.gz | 51.9 KB | Display | PDBx/mmCIF format |
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PDB format | pdb4fhq.ent.gz | 37.2 KB | Display | PDB format |
PDBx/mmJSON format | 4fhq.json.gz | Tree view | PDBx/mmJSON format | |
Others | Other downloads |
-Validation report
Arichive directory | https://data.pdbj.org/pub/pdb/validation_reports/fh/4fhq ftp://data.pdbj.org/pub/pdb/validation_reports/fh/4fhq | HTTPS FTP |
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-Related structure data
Related structure data | 2aw2S S: Starting model for refinement |
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Similar structure data | |
Other databases |
-Links
-Assembly
Deposited unit |
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1 |
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Unit cell |
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Details | AUTHOR DETERMINED BIOLOGICAL UNIT: NOT DETERMINED |
-Components
#1: Protein | Mass: 14332.176 Da / Num. of mol.: 1 / Fragment: extracellular domain residues 39-162 Source method: isolated from a genetically manipulated source Source: (gene. exp.) Homo sapiens (human) / Gene: HVEA, HVEM, TNFRSF14, UNQ329/PRO509 / Plasmid: pMT-Bip-His / Cell (production host): Schneider 2 / Production host: Drosophila melanogaster (fruit fly) / References: UniProt: Q92956 |
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#2: Water | ChemComp-HOH / |
-Experimental details
-Experiment
Experiment | Method: X-RAY DIFFRACTION / Number of used crystals: 1 |
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-Sample preparation
Crystal | Density Matthews: 3 Å3/Da / Density % sol: 58.99 % |
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Crystal grow | Temperature: 294 K / Method: vapor diffusion, sitting drop / pH: 8.5 Details: 0.2 M SODIUM ACETATE TRIHYDRATE, PH 8.5, TRIS-HCL, 30% W/V PEG4000, VAPOR DIFFUSION, SITTING DROP, temperature 294.0K |
-Data collection
Diffraction | Mean temperature: 100 K |
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Diffraction source | Source: SYNCHROTRON / Site: NSLS / Beamline: X29A / Wavelength: 1.075 / Wavelength: 1.075 Å |
Detector | Type: ADSC QUANTUM 315 / Detector: CCD / Date: Mar 15, 2012 / Details: MIRRORS |
Radiation | Protocol: SINGLE WAVELENGTH / Monochromatic (M) / Laue (L): M / Scattering type: x-ray |
Radiation wavelength | Wavelength: 1.075 Å / Relative weight: 1 |
Reflection | Resolution: 2.25→50 Å / Num. obs: 8551 / % possible obs: 99.3 % / Redundancy: 7.6 % / Biso Wilson estimate: 54.17 Å2 / Rmerge(I) obs: 0.104 / Rsym value: 0.09 / Net I/σ(I): 16.68 |
Reflection shell | Resolution: 2.25→2.29 Å / Redundancy: 6.4 % / Mean I/σ(I) obs: 1.062 / % possible all: 94.7 |
-Processing
Software |
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Refinement | Method to determine structure: MOLECULAR REPLACEMENT Starting model: 2AW2 Resolution: 2.251→33.494 Å / SU ML: 0.47 / σ(F): 0 / Phase error: 38.13 / Stereochemistry target values: MLHL
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Solvent computation | Shrinkage radii: 0.6 Å / VDW probe radii: 0.9 Å / Solvent model: FLAT BULK SOLVENT MODEL / Bsol: 66.537 Å2 / ksol: 0.359 e/Å3 | |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Displacement parameters |
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Refinement step | Cycle: LAST / Resolution: 2.251→33.494 Å
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Refine LS restraints |
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LS refinement shell |
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Refinement TLS params. | Method: refined / Refine-ID: X-RAY DIFFRACTION
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Refinement TLS group |
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