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- PDB-3wue: The wild type crystal structure of b-1,4-Xylanase (XynAS9) with x... -
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Open data
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Basic information
Entry | Database: PDB / ID: 3wue | |||||||||
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Title | The wild type crystal structure of b-1,4-Xylanase (XynAS9) with xylobiose from Streptomyces sp. 9 | |||||||||
![]() | Endo-1,4-beta-xylanase A | |||||||||
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Function / homology | ![]() ![]() ![]() ![]() Similarity search - Function | |||||||||
Biological species | ![]() | |||||||||
Method | ![]() ![]() ![]() | |||||||||
![]() | Chen, C.C. / Han, X. / Lv, P. / Ko, T.P. / Peng, W. / Huang, C.H. / Zheng, Y. / Gao, J. / Yang, Y. / Guo, R.T. | |||||||||
![]() | ![]() Title: Structural perspectives of an engineered beta-1,4-xylanase with enhanced thermostability. Authors: Chen, C.C. / Luo, H. / Han, X. / Lv, P. / Ko, T.P. / Peng, W. / Huang, C.H. / Wang, K. / Gao, J. / Zheng, Y. / Yang, Y. / Zhang, J. / Yao, B. / Guo, R.T. | |||||||||
History |
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Structure visualization
Structure viewer | Molecule: ![]() ![]() |
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Downloads & links
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Download
PDBx/mmCIF format | ![]() | 81.9 KB | Display | ![]() |
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PDB format | ![]() | 58.8 KB | Display | ![]() |
PDBx/mmJSON format | ![]() | Tree view | ![]() | |
Others | ![]() |
-Validation report
Arichive directory | ![]() ![]() | HTTPS FTP |
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-Related structure data
Related structure data | ![]() 3wubC ![]() 3wufC ![]() 3wugSC C: citing same article ( S: Starting model for refinement |
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Similar structure data |
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Links
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Assembly
Deposited unit | ![]()
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1 |
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Unit cell |
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Components
#1: Protein | Mass: 34611.449 Da / Num. of mol.: 1 / Fragment: UNP RESIDUES 39-351 Source method: isolated from a genetically manipulated source Source: (gene. exp.) ![]() ![]() ![]() ![]() | ||||
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#2: Polysaccharide | #3: Chemical | ChemComp-ZN / #4: Water | ChemComp-HOH / | ![]() |
-Experimental details
-Experiment
Experiment | Method: ![]() |
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Sample preparation
Crystal | Density Matthews: 3.94 Å3/Da / Density % sol: 68.8 % |
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Crystal grow![]() | Temperature: 298 K / Method: vapor diffusion, sitting drop / pH: 6.5 Details: 0.5M zinc acetate dehydrate, 0.1M sodium cacodylate trihydrate pH 6.5, 8% (w/v) polyethylene glycol 8000, VAPOR DIFFUSION, SITTING DROP, temperature 298K |
-Data collection
Diffraction | Mean temperature: 100 K |
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Diffraction source | Source: ![]() ![]() ![]() |
Detector | Type: ADSC QUANTUM 315 / Detector: CCD / Date: Jun 18, 2013 |
Radiation | Protocol: SINGLE WAVELENGTH / Monochromatic (M) / Laue (L): M / Scattering type: x-ray |
Radiation wavelength | Wavelength![]() |
Reflection | Resolution: 2.15→25 Å / Num. obs: 31458 / % possible obs: 99.8 % / Redundancy: 19.5 % / Rmerge(I) obs: 0.084 / Net I/σ(I): 46.1 |
Reflection shell | Resolution: 2.15→2.23 Å / Redundancy: 20.9 % / Rmerge(I) obs: 0.475 / Mean I/σ(I) obs: 10.3 / % possible all: 100 |
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Processing
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Refinement | Method to determine structure![]() ![]() Starting model: 3WUG Resolution: 2.15→25 Å / Stereochemistry target values: Engh & Huber
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Refinement step | Cycle: LAST / Resolution: 2.15→25 Å
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LS refinement shell | Resolution: 2.15→2.23 Å /
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