Journal: To be Published Title: Purification, cloning, functional expression, structure, and characterization of a thermostable beta-mannanase from Talaromyces trachyspermus and its efficiency in production of ...Title: Purification, cloning, functional expression, structure, and characterization of a thermostable beta-mannanase from Talaromyces trachyspermus and its efficiency in production of mannooligosaccharides from coffee wastes Authors: Ichinose, H. / Suzuki, K. / Yuki, M. / Michikawa, M. / Sato, H. / Kamino, K. / Ogasawara, W. / Fushinobu, S. / Kaneko, S.
Resolution: 1.6→19.28 Å / Cor.coef. Fo:Fc: 0.973 / Cor.coef. Fo:Fc free: 0.964 / SU B: 0.796 / SU ML: 0.029 / Cross valid method: THROUGHOUT / ESU R: 0.058 / ESU R Free: 0.06 / Stereochemistry target values: MAXIMUM LIKELIHOOD / Details: HYDROGENS HAVE BEEN ADDED IN THE RIDING POSITIONS
Rfactor
Num. reflection
% reflection
Selection details
Rfree
0.13792
2389
5.1 %
RANDOM
Rwork
0.11368
-
-
-
all
0.11492
45131
-
-
obs
0.11492
44734
99.12 %
-
Solvent computation
Ion probe radii: 0.8 Å / Shrinkage radii: 0.8 Å / VDW probe radii: 1.2 Å / Solvent model: BABINET MODEL WITH MASK