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Yorodumi- PDB-3pdz: SOLUTION STRUCTURE OF THE PDZ2 DOMAIN FROM HUMAN PHOSPHATASE HPTP1E -
+Open data
-Basic information
Entry | Database: PDB / ID: 3pdz | ||||||
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Title | SOLUTION STRUCTURE OF THE PDZ2 DOMAIN FROM HUMAN PHOSPHATASE HPTP1E | ||||||
Components | PROTEIN (TYROSINE PHOSPHATASE (PTP-BAS, TYPE 1)) | ||||||
Keywords | HYDROLASE / PDZ DOMAIN / HUMAN PHOSPHATASE / HPTP1E / PTP-BAS / SPECIFICITY OF BINDING | ||||||
Function / homology | Function and homology information negative regulation of excitatory synapse assembly / cellular response to toxic substance / phosphatidylinositol 3-kinase regulatory subunit binding / Interleukin-37 signaling / RND1 GTPase cycle / RND2 GTPase cycle / RND3 GTPase cycle / regulation of phosphatidylinositol 3-kinase/protein kinase B signal transduction / Synthesis of PIPs at the plasma membrane / peptidyl-tyrosine dephosphorylation ...negative regulation of excitatory synapse assembly / cellular response to toxic substance / phosphatidylinositol 3-kinase regulatory subunit binding / Interleukin-37 signaling / RND1 GTPase cycle / RND2 GTPase cycle / RND3 GTPase cycle / regulation of phosphatidylinositol 3-kinase/protein kinase B signal transduction / Synthesis of PIPs at the plasma membrane / peptidyl-tyrosine dephosphorylation / protein dephosphorylation / protein-tyrosine-phosphatase / negative regulation of protein phosphorylation / protein tyrosine phosphatase activity / fibrillar center / lamellipodium / cell body / cytoskeleton / extracellular exosome / nucleoplasm / nucleus / plasma membrane / cytosol / cytoplasm Similarity search - Function | ||||||
Biological species | Homo sapiens (human) | ||||||
Method | SOLUTION NMR / simulated annealing | ||||||
Authors | Kozlov, G. / Gehring, K. / Ekiel, I. | ||||||
Citation | Journal: Biochemistry / Year: 2000 Title: Solution structure of the PDZ2 domain from human phosphatase hPTP1E and its interactions with C-terminal peptides from the Fas receptor. Authors: Kozlov, G. / Gehring, K. / Ekiel, I. | ||||||
History |
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-Structure visualization
Structure viewer | Molecule: MolmilJmol/JSmol |
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-Downloads & links
-Download
PDBx/mmCIF format | 3pdz.cif.gz | 827.4 KB | Display | PDBx/mmCIF format |
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PDB format | pdb3pdz.ent.gz | 690.9 KB | Display | PDB format |
PDBx/mmJSON format | 3pdz.json.gz | Tree view | PDBx/mmJSON format | |
Others | Other downloads |
-Validation report
Arichive directory | https://data.pdbj.org/pub/pdb/validation_reports/pd/3pdz ftp://data.pdbj.org/pub/pdb/validation_reports/pd/3pdz | HTTPS FTP |
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-Related structure data
Similar structure data |
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-Links
-Assembly
Deposited unit |
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1 |
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NMR ensembles |
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-Components
#1: Protein | Mass: 10020.252 Da / Num. of mol.: 1 / Fragment: PDZ2 DOMAIN Source method: isolated from a genetically manipulated source Source: (gene. exp.) Homo sapiens (human) / Species (production host): Escherichia coli / Production host: Escherichia coli BL21 (bacteria) / Strain (production host): BL21 / References: UniProt: Q12923, protein-tyrosine-phosphatase |
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-Experimental details
-Experiment
Experiment | Method: SOLUTION NMR | ||||||||||||||||
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NMR experiment |
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NMR details | Text: THE STRUCTURE WAS DETERMINED USING 2D AND 3D EXPERIMENTS USING UNLABELED, 15N- LABELED, AND DOUBLE-LABELED PROTEIN |
-Sample preparation
Details | Contents: 10% D2O/90% WATER |
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Sample conditions | pH: 6.8 / Pressure: 1 atm / Temperature: 293 K |
Crystal grow | *PLUS Method: other / Details: NMR |
-NMR measurement
NMR spectrometer | Type: Bruker DMX500 / Manufacturer: Bruker / Model: DMX500 / Field strength: 500 MHz |
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-Processing
NMR software |
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Refinement | Method: simulated annealing / Software ordinal: 1 Details: REFINEMENT DETAILS CAN BE FOUND IN PRIMARY REFERENCE | |||||||||
NMR ensemble | Conformer selection criteria: LOWEST ENERGY / Conformers calculated total number: 200 / Conformers submitted total number: 30 |