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Open data
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Basic information
Entry | Database: PDB / ID: 3ozz | ||||||
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Title | Structure of a cytochrome b5 core-swap mutant | ||||||
![]() | Cytochrome b5![]() | ||||||
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Function / homology | ![]() Vitamin C (ascorbate) metabolism / Insertion of tail-anchored proteins into the endoplasmic reticulum membrane / mitochondrial outer membrane / intracellular membrane-bounded organelle / ![]() ![]() Similarity search - Function | ||||||
Biological species | ![]() ![]() ![]() | ||||||
Method | ![]() ![]() | ||||||
![]() | Terzyan, S. / Parthasarathy, S. / Zhang, X.C. / Benson, D. | ||||||
![]() | ![]() Title: Structure of a cytochrome b5 core-swap mutant Authors: Parthasarathy, S. / Terzyan, S. / Zhang, X.C. / Benson, D. | ||||||
History |
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Structure visualization
Structure viewer | Molecule: ![]() ![]() |
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Downloads & links
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Download
PDBx/mmCIF format | ![]() | 36.6 KB | Display | ![]() |
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PDB format | ![]() | 24 KB | Display | ![]() |
PDBx/mmJSON format | ![]() | Tree view | ![]() | |
Others | ![]() |
-Validation report
Arichive directory | ![]() ![]() | HTTPS FTP |
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-Related structure data
Related structure data | ![]() 1awpS S: Starting model for refinement |
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Similar structure data |
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Links
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Assembly
Deposited unit | ![]()
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Unit cell |
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Components on special symmetry positions |
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Components
#1: Protein | ![]() Mass: 9446.489 Da / Num. of mol.: 1 Fragment: N-TERMINAL HEME-BINDING DOMAIN (unp residues 8-89) Source method: isolated from a genetically manipulated source Source: (gene. exp.) ![]() ![]() ![]() ![]() ![]() ![]() |
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#2: Chemical | ChemComp-HEM / ![]() |
#3: Water | ChemComp-HOH / ![]() |
-Experimental details
-Experiment
Experiment | Method: ![]() |
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Sample preparation
Crystal | Density Matthews: 2.1 Å3/Da / Density % sol: 41.55 % |
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Crystal grow![]() | Temperature: 277 K / Method: vapor diffusion / pH: 7 Details: 30% PEG8000, 0.2M MgAc, 0.1M Pipes, pH 7.0, VAPOR DIFFUSION, temperature 277K |
-Data collection
Diffraction | Mean temperature: 80 K |
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Diffraction source | Source: ![]() |
Detector | Type: MAR scanner 345 mm plate / Detector: IMAGE PLATE / Date: Jan 1, 2007 / Details: mirrors |
Radiation | Monochromator: Osmic Blue optics / Protocol: SINGLE WAVELENGTH / Monochromatic (M) / Laue (L): M / Scattering type: x-ray |
Radiation wavelength | Wavelength![]() |
Reflection | Resolution: 1.7→50 Å / Num. obs: 8707 / % possible obs: 98.4 % / Observed criterion σ(F): -3 / Redundancy: 4 % / Biso Wilson estimate: 17.87 Å2 / Rmerge(I) obs: 0.05 / Net I/σ(I): 24.5 |
Reflection shell | Resolution: 1.7→1.76 Å / Rmerge(I) obs: 0.152 / Mean I/σ(I) obs: 8.2 / % possible all: 95.3 |
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Processing
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Refinement | Method to determine structure![]() ![]() Starting model: PDB ENTRY 1AWP Resolution: 1.7→50 Å / Isotropic thermal model: Anisotropic Overall Temp. factor / σ(F): 0 / Stereochemistry target values: Engh & Huber
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Displacement parameters | Biso mean: 17.72 Å2
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Refinement step | Cycle: LAST / Resolution: 1.7→50 Å
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Refine LS restraints |
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