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- PDB-3ol0: Crystal structure of Monofoil-4P homo-trimer: de novo designed mo... -
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Open data
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Basic information
Entry | Database: PDB / ID: 3ol0 | ||||||
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Title | Crystal structure of Monofoil-4P homo-trimer: de novo designed monomer trefoil-fold sub-domain which forms homo-trimer assembly | ||||||
![]() | de novo designed monomer trefoil-fold sub-domain which forms homo-trimer assembly | ||||||
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Function / homology | SH3 type barrels. - #30 / SH3 type barrels. / Other non-globular / Special![]() | ||||||
Biological species | synthetic construct (others) | ||||||
Method | ![]() ![]() ![]() | ||||||
![]() | Lee, J. / Blaber, M. | ||||||
![]() | ![]() Title: Experimental support for the evolution of symmetric protein architecture from a simple peptide motif. Authors: Lee, J. / Blaber, M. | ||||||
History |
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Structure visualization
Structure viewer | Molecule: ![]() ![]() |
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Downloads & links
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Download
PDBx/mmCIF format | ![]() | 42.7 KB | Display | ![]() |
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PDB format | ![]() | 30.8 KB | Display | ![]() |
PDBx/mmJSON format | ![]() | Tree view | ![]() | |
Others | ![]() |
-Validation report
Arichive directory | ![]() ![]() | HTTPS FTP |
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-Related structure data
Related structure data | ![]() 3o49C ![]() 3o4aC ![]() 3o4bC ![]() 3o4cC ![]() 3o4dC ![]() 3ogfC C: citing same article ( |
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Similar structure data |
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Links
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Assembly
Deposited unit | ![]()
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Unit cell |
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Components
#1: Protein/peptide | Mass: 5443.896 Da / Num. of mol.: 3 Source method: isolated from a genetically manipulated source Details: Synthetic sequence derived from human acidic fibroblast growth factor with a symmetric deconstruction method. The protein produced by this sequence forms a monomer trefoil-fold sub-domain ...Details: Synthetic sequence derived from human acidic fibroblast growth factor with a symmetric deconstruction method. The protein produced by this sequence forms a monomer trefoil-fold sub-domain and exists as a homo-trimer assembly adopting a beta-trefoil architecture. Source: (gene. exp.) synthetic construct (others) / Production host: ![]() ![]() ![]() #2: Chemical | ChemComp-SO4 / ![]() #3: Water | ChemComp-HOH / | ![]() |
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-Experimental details
-Experiment
Experiment | Method: ![]() |
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Sample preparation
Crystal | Density Matthews: 2.67 Å3/Da / Density % sol: 53.94 % |
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Crystal grow![]() | Temperature: 298 K / Method: vapor diffusion, hanging drop / pH: 5.5 Details: 2M AMMONIUM SULFATE, 0.1M NA CITRATE, 15MG/ML PROTEIN CONCENTRATION, Ph 5.5, VAPOR DIFFUSION, HANGING DROP, TEMPERATURE 298K |
-Data collection
Diffraction | Mean temperature: 100 K |
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Diffraction source | Source: ![]() ![]() ![]() |
Detector | Type: ADSC QUANTUM 315 / Detector: CCD / Date: Aug 23, 2010 |
Radiation | Monochromator: SI / Protocol: SINGLE WAVELENGTH / Monochromatic (M) / Laue (L): M / Scattering type: x-ray |
Radiation wavelength | Wavelength![]() |
Reflection | Resolution: 1.48→50 Å / Num. all: 29633 / Num. obs: 29337 / % possible obs: 99 % / Observed criterion σ(F): 3 / Observed criterion σ(I): 3 / Redundancy: 19.4 % / Rmerge(I) obs: 0.069 / Net I/σ(I): 82.4 |
Reflection shell | Resolution: 1.48→1.51 Å / Redundancy: 16.6 % / Rmerge(I) obs: 0.358 / Mean I/σ(I) obs: 8.38 / Num. unique all: 1434 / % possible all: 98.8 |
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Processing
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Refinement | Method to determine structure![]() ![]()
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Solvent computation | Shrinkage radii: 0.9 Å / VDW probe radii: 1.11 Å / Solvent model: FLAT BULK SOLVENT MODEL / Bsol: 37.175 Å2 / ksol: 0.376 e/Å3 | |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Displacement parameters |
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Refinement step | Cycle: LAST / Resolution: 1.483→41.517 Å
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Refine LS restraints |
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LS refinement shell | Refine-ID: X-RAY DIFFRACTION
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