+Open data
-Basic information
Entry | Database: PDB / ID: 3now | ||||||
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Title | UNC-45 from Drosophila melanogaster | ||||||
Components | UNC-45 protein, SD10334p | ||||||
Keywords | PROTEIN BINDING / Armadillo Repeat / Hsp90 / Myosin / Tetra-tricopeptide Repeat / TPR / binding protein required for myosin function | ||||||
Function / homology | Function and homology information somatic muscle development / myosin filament assembly / muscle organ development / chaperone-mediated protein folding / negative regulation of innate immune response / Hsp90 protein binding / cellular response to heat / defense response to Gram-negative bacterium / cell differentiation / cytoplasm Similarity search - Function | ||||||
Biological species | Drosophila melanogaster (fruit fly) | ||||||
Method | X-RAY DIFFRACTION / SYNCHROTRON / SAD / Resolution: 2.992 Å | ||||||
Authors | Lee, C.F. / Hauenstein, A.V. / Fleming, J.K. / Gasper, W.C. / Engelke, V. / Banumathi, S. / Bernstein, S.I. / Huxford, T. | ||||||
Citation | Journal: Structure / Year: 2011 Title: X-ray Crystal Structure of the UCS Domain-Containing UNC-45 Myosin Chaperone from Drosophila melanogaster. Authors: Lee, C.F. / Hauenstein, A.V. / Fleming, J.K. / Gasper, W.C. / Engelke, V. / Sankaran, B. / Bernstein, S.I. / Huxford, T. | ||||||
History |
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-Structure visualization
Structure viewer | Molecule: MolmilJmol/JSmol |
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-Downloads & links
-Download
PDBx/mmCIF format | 3now.cif.gz | 161.5 KB | Display | PDBx/mmCIF format |
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PDB format | pdb3now.ent.gz | 127 KB | Display | PDB format |
PDBx/mmJSON format | 3now.json.gz | Tree view | PDBx/mmJSON format | |
Others | Other downloads |
-Validation report
Arichive directory | https://data.pdbj.org/pub/pdb/validation_reports/no/3now ftp://data.pdbj.org/pub/pdb/validation_reports/no/3now | HTTPS FTP |
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-Related structure data
Similar structure data |
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-Links
-Assembly
Deposited unit |
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1 |
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2 |
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Unit cell |
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-Components
#1: Protein | Mass: 89813.523 Da / Num. of mol.: 1 / Fragment: UNP residues 138-947 Source method: isolated from a genetically manipulated source Source: (gene. exp.) Drosophila melanogaster (fruit fly) / Gene: CG2708, Tom34 / Plasmid: pET16b / Production host: Escherichia coli (E. coli) / Strain (production host): Rosetta (DE3) pLysS / References: UniProt: Q960B1 |
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-Experimental details
-Experiment
Experiment | Method: X-RAY DIFFRACTION / Number of used crystals: 1 |
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-Sample preparation
Crystal | Density Matthews: 5.79 Å3/Da / Density % sol: 78.7 % |
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Crystal grow | Temperature: 291 K / Method: vapor diffusion, hanging drop / pH: 5.6 Details: 0.1 M sodium citrate, 0.2 M ammonium acetate, 25% (w/v) PEG 4000, 1% (v/v) ethylene glycol, pH 5.6, VAPOR DIFFUSION, HANGING DROP, temperature 291.0K |
-Data collection
Diffraction | Mean temperature: 100 K |
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Diffraction source | Source: SYNCHROTRON / Site: ALS / Beamline: 8.2.2 / Wavelength: 1 Å |
Detector | Type: ADSC QUANTUM 315 / Detector: CCD / Date: Aug 10, 2007 |
Radiation | Scattering type: x-ray |
Radiation wavelength | Wavelength: 1 Å / Relative weight: 1 |
Reflection | Resolution: 2.992→50 Å / Num. obs: 42099 / % possible obs: 99.8 % / Observed criterion σ(F): 2 / Observed criterion σ(I): 2 / Redundancy: 9.5 % / Rsym value: 0.0115 / Net I/σ(I): 19.2 |
Reflection shell | Resolution: 2.992→3.11 Å / Redundancy: 6.5 % / Num. unique all: 4113 / % possible all: 97.8 |
-Processing
Software |
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Refinement | Method to determine structure: SAD / Resolution: 2.992→49.197 Å / SU ML: 0.45 / σ(F): 0.15 / Stereochemistry target values: ML
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Solvent computation | Shrinkage radii: 0.9 Å / VDW probe radii: 1.11 Å / Solvent model: FLAT BULK SOLVENT MODEL / Bsol: 20.07 Å2 / ksol: 0.292 e/Å3 | |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Displacement parameters |
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Refinement step | Cycle: LAST / Resolution: 2.992→49.197 Å
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Refine LS restraints |
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LS refinement shell |
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