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Yorodumi- PDB-3nbb: Crystal structure of mutant Y305F expressed in E. coli in the cop... -
+Open data
-Basic information
Entry | Database: PDB / ID: 3nbb | ||||||
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Title | Crystal structure of mutant Y305F expressed in E. coli in the copper amine oxidase from hansenula polymorpha | ||||||
Components | Peroxisomal primary amine oxidase | ||||||
Keywords | OXIDOREDUCTASE / amine oxidase / quinoprotein | ||||||
Function / homology | Function and homology information : / : / : / primary-amine oxidase / amine metabolic process / quinone binding / peroxisome / copper ion binding Similarity search - Function | ||||||
Biological species | Pichia angusta (fungus) | ||||||
Method | X-RAY DIFFRACTION / SYNCHROTRON / MOLECULAR REPLACEMENT / Resolution: 2.05 Å | ||||||
Authors | Chen, Z. / Datta, S. / DuBois, J.L. / Klinman, J.P. / Mathews, F.S. | ||||||
Citation | Journal: Biochemistry / Year: 2010 Title: Mutation at a strictly conserved, active site tyrosine in the copper amine oxidase leads to uncontrolled oxygenase activity. Authors: Chen, Z.W. / Datta, S. / Dubois, J.L. / Klinman, J.P. / Mathews, F.S. #1: Journal: Structure / Year: 1998 Title: Copper amine oxidase from Hansenula polymorpha: the crystal structure determined at 2.4 A resolution reveals the active conformation. Authors: Li, R. / Klinman, J.P. / Mathews, F.S. | ||||||
History |
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-Structure visualization
Structure viewer | Molecule: MolmilJmol/JSmol |
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-Downloads & links
-Download
PDBx/mmCIF format | 3nbb.cif.gz | 831.6 KB | Display | PDBx/mmCIF format |
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PDB format | pdb3nbb.ent.gz | 689.1 KB | Display | PDB format |
PDBx/mmJSON format | 3nbb.json.gz | Tree view | PDBx/mmJSON format | |
Others | Other downloads |
-Validation report
Arichive directory | https://data.pdbj.org/pub/pdb/validation_reports/nb/3nbb ftp://data.pdbj.org/pub/pdb/validation_reports/nb/3nbb | HTTPS FTP |
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-Related structure data
Related structure data | 3n9hC 3nbjC 1a2vS S: Starting model for refinement C: citing same article (ref.) |
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Similar structure data |
-Links
-Assembly
Deposited unit |
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Unit cell |
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-Components
#1: Protein | Mass: 77696.617 Da / Num. of mol.: 6 / Mutation: Y305F Source method: isolated from a genetically manipulated source Source: (gene. exp.) Pichia angusta (fungus) / Gene: AMO / Plasmid: PET11A / Production host: Escherichia coli (E. coli) / Strain (production host): BL21 / References: UniProt: P12807, primary-amine oxidase #2: Chemical | ChemComp-CU / #3: Water | ChemComp-HOH / | |
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-Experimental details
-Experiment
Experiment | Method: X-RAY DIFFRACTION / Number of used crystals: 1 |
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-Sample preparation
Crystal | Density Matthews: 2.52 Å3/Da / Density % sol: 51.13 % |
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Crystal grow | Temperature: 295 K / pH: 7.5 Details: 20% PEG8000, 100mM HEPES, 2% ethylene glycol, pH 7.5, VAPOR DIFFUSION, HANGING DROP, temperature 295K |
-Data collection
Diffraction | Mean temperature: 100 K |
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Diffraction source | Source: SYNCHROTRON / Site: APS / Beamline: 14-BM-C / Wavelength: 0.9 |
Detector | Type: ADSC QUANTUM 4 / Detector: CCD / Date: Nov 5, 2003 |
Radiation | Monochromator: BENT GE(111) MONOCHROMATOR / Protocol: SINGLE WAVELENGTH / Monochromatic (M) / Laue (L): M / Scattering type: x-ray |
Radiation wavelength | Wavelength: 0.9 Å / Relative weight: 1 |
Reflection | Resolution: 2.05→40 Å / Num. obs: 217301 / % possible obs: 77.4 % / Observed criterion σ(I): -3 / Redundancy: 3.2 % / Biso Wilson estimate: 7.7 Å2 / Rmerge(I) obs: 0.067 / Net I/σ(I): 14.2 |
Reflection shell | Resolution: 2.05→2.12 Å / Redundancy: 3.1 % / Rmerge(I) obs: 0.318 / Mean I/σ(I) obs: 3.3 |
-Processing
Software |
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Refinement | Method to determine structure: MOLECULAR REPLACEMENT Starting model: PDB ENTRY 1A2V Resolution: 2.05→36.13 Å / Rfactor Rfree error: 0.002 / Data cutoff high absF: 217092.7 / Data cutoff low absF: 0 / Isotropic thermal model: ISOTROPIC / Cross valid method: THROUGHOUT / σ(F): 0 / Stereochemistry target values: ENGH & HUBER
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Displacement parameters | Biso mean: 29.7 Å2 | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Refine analyze |
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Refinement step | Cycle: LAST / Resolution: 2.05→36.13 Å
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Refine LS restraints |
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LS refinement shell | Resolution: 2.05→2.18 Å / Rfactor Rfree error: 0.008 / Total num. of bins used: 6
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Xplor file | Serial no: 1 / Param file: protein_rep_tpo405.par / Topol file: protein_tpo405.top |