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Yorodumi- PDB-3ko5: D-Tyr-tRNA(Tyr) deacylase from Plasmodium falciparum in complex w... -
+Open data
-Basic information
Entry | Database: PDB / ID: 3ko5 | ||||||
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Title | D-Tyr-tRNA(Tyr) deacylase from Plasmodium falciparum in complex with ADP | ||||||
Components | D-tyrosyl-tRNA(Tyr) deacylase | ||||||
Keywords | HYDROLASE / DTD / ADP / Deacylase | ||||||
Function / homology | Function and homology information Gly-tRNA(Ala) hydrolase activity / D-tyrosyl-tRNA(Tyr) deacylase activity / D-aminoacyl-tRNA deacylase / tRNA metabolic process / tRNA binding / nucleotide binding / cytoplasm Similarity search - Function | ||||||
Biological species | Plasmodium falciparum (malaria parasite P. falciparum) | ||||||
Method | X-RAY DIFFRACTION / SYNCHROTRON / MOLECULAR REPLACEMENT / molecular replacement / Resolution: 2.09 Å | ||||||
Authors | Manickam, Y. / Bhatt, T.K. / Sharma, A. | ||||||
Citation | Journal: J.Biol.Chem. / Year: 2010 Title: Ligand-bound Structures Provide Atomic Snapshots for the Catalytic Mechanism of D-Amino Acid Deacylase Authors: Bhatt, T.K. / Yogavel, M. / Wydau, S. / Berwal, R. / Sharma, A. | ||||||
History |
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-Structure visualization
Structure viewer | Molecule: MolmilJmol/JSmol |
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-Downloads & links
-Download
PDBx/mmCIF format | 3ko5.cif.gz | 207.3 KB | Display | PDBx/mmCIF format |
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PDB format | pdb3ko5.ent.gz | 166.6 KB | Display | PDB format |
PDBx/mmJSON format | 3ko5.json.gz | Tree view | PDBx/mmJSON format | |
Others | Other downloads |
-Validation report
Arichive directory | https://data.pdbj.org/pub/pdb/validation_reports/ko/3ko5 ftp://data.pdbj.org/pub/pdb/validation_reports/ko/3ko5 | HTTPS FTP |
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-Related structure data
Related structure data | 3knfC 3knpC 3ko3C 3ko4C 3ko7C 3ko9C 3kobC 3kocC 3kodC C: citing same article (ref.) |
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Similar structure data |
-Links
-Assembly
Deposited unit |
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2 |
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3 |
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Unit cell |
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-Components
#1: Protein | Mass: 19233.084 Da / Num. of mol.: 6 Source method: isolated from a genetically manipulated source Source: (gene. exp.) Plasmodium falciparum (malaria parasite P. falciparum) Strain: 3D7 / Gene: dtd / Plasmid: pET28a / Production host: Escherichia coli (E. coli) / Strain (production host): b834(DE3) References: UniProt: Q8IIS0, Hydrolases; Acting on ester bonds #2: Chemical | ChemComp-ADP / #3: Water | ChemComp-HOH / | |
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-Experimental details
-Experiment
Experiment | Method: X-RAY DIFFRACTION / Number of used crystals: 1 |
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-Sample preparation
Crystal | Density Matthews: 2.05 Å3/Da / Density % sol: 40.05 % / Mosaicity: 1.548 ° |
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Crystal grow | Temperature: 293 K / Method: vapor diffusion, hanging drop Details: 25% PEG 3350, 0.1M MES, pH 6.2-6.5, VAPOR DIFFUSION, HANGING DROP, temperature 293K PH range: 6.2-6.5 |
-Data collection
Diffraction | Mean temperature: 100 K |
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Diffraction source | Source: SYNCHROTRON / Site: ESRF / Beamline: BM14 / Wavelength: 0.97 Å |
Detector | Type: MARMOSAIC 225 mm CCD / Detector: CCD / Date: Dec 13, 2007 |
Radiation | Protocol: SINGLE WAVELENGTH / Monochromatic (M) / Laue (L): M / Scattering type: x-ray |
Radiation wavelength | Wavelength: 0.97 Å / Relative weight: 1 |
Reflection | Resolution: 2.09→25 Å / Num. obs: 52153 / % possible obs: 97.1 % / Redundancy: 2.1 % / Rmerge(I) obs: 0.06 / Χ2: 0.782 / Net I/σ(I): 7.5 |
Reflection shell | Resolution: 2.1→2.18 Å / Redundancy: 1.9 % / Rmerge(I) obs: 0.446 / Num. unique all: 5054 / Χ2: 0.849 / % possible all: 94 |
-Phasing
Phasing | Method: molecular replacement |
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-Processing
Software |
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Refinement | Method to determine structure: MOLECULAR REPLACEMENT Starting model: PfDTD-Iodide model (not deposited) Resolution: 2.09→25 Å / Cor.coef. Fo:Fc: 0.945 / Cor.coef. Fo:Fc free: 0.897 / Occupancy max: 1 / Occupancy min: 0.3 / SU B: 6.931 / SU ML: 0.185 / Cross valid method: THROUGHOUT / σ(F): 0 / ESU R: 0.336 / ESU R Free: 0.247 / Stereochemistry target values: MAXIMUM LIKELIHOOD
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Solvent computation | Ion probe radii: 0.8 Å / Shrinkage radii: 0.8 Å / VDW probe radii: 1.2 Å / Solvent model: MASK | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Displacement parameters | Biso max: 81.65 Å2 / Biso mean: 28.69 Å2 / Biso min: 10.73 Å2
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Refinement step | Cycle: LAST / Resolution: 2.09→25 Å
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Refine LS restraints |
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LS refinement shell | Resolution: 2.094→2.148 Å / Total num. of bins used: 20
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